Research Article Genetic diversity in a Poincianella pyramidalis (Tul.) L.P. Queiroz population assessed by RAPD molecular markers.

Abstract

ABSTRACT. Poincianella pyramidalis (Tul.) L.P. Queiroz is an endemic Caatinga (Brazilian savannah biome) species that has been exploited for different purposes, although information is necessary about still existing natural populations. The objective of this study was to evaluate the genetic diversity among 20 P. pyramidalis individuals occurring in a population localized in the Caatinga biome of Paraíba State, aiming at seed collection, using RAPD markers. For the DNA extraction, young shoots of the individuals were used, and amplification was carried out using 20 primers. The obtained markers were converted to a binary matrix, from which a genetic dissimilarity matrix was built using the arithmetic complement of Jaccard's coefficient, and the dendrogram was built by the UPGMA analysis. No amplified fragment was monomorphic, resulting in 100% polymorphism of the analyzed population. The mean genetic diversity among the matrices was 63.28%, ranging from 30.9 to 97.7%. Individuals 09 and 17 showed relevant genetic proximity, and thus planting their seedlings at close sites would not be indicated. The population evaluated in this study showed high genetic diversity, originating twelve groups from the UPGMA hierarchical cluster analysis. Based on the results, individuals 09 and 17 can provide plant material for the evaluation of the physiological performance of P. pyramidalis seeds, and the set of individuals of this population has a high genetic diversity that characterizes them as adequate matrices for projects of restoration and conservation of the seed species.

SEED QUALITY AND GENETIC DIVERSITY OF A CULTIVATED POPULATION OF Mimosa caesalpiniifolia BENTH

“…These estimates allow seed collection in the most genetically distant trees. Trees with desirable properties and genetically divergent in the population should be selected for seed collection, since they are essential for the formation of seed lots with greater genetic diversity (BELARMINO et al, 2017), as the more divergent the selected parents, the greater the variability of their progeny (MANFIO et al, 2012). This is of great value when the seeds are intended for forest recovery and germplasm conservation (SEBBENN, 2002).…”

“…In Brazil, the genetic characterisation of seed source populations of native trees is routinely performed using genetic markers, such as morphological (COSTA et al, 2016;ROVERI NETO;PAULA, 2017;CORREIA et al, 2019;FELIX et al, 2020) and/or DNA (or molecular) analysis. Commonly used markers are Random Amplified Polymorphic DNA (RAPD) (RABBANI; SILVA-MANN;FERREIRA, 2012;BELARMINO et al, 2017;ALMEIDA NETO et al, 2019) and Inter Simple Sequence Repeats (ISSR) (DUARTE;VIEIRA, 2018;SILVA JÚNIOR et al, 2017). Of these, ISSR markers are more accessible and do not require prior knowledge of the genome of species to design primers, which is still small for native Brazilian tree species.…”

Assessment of plant genetic variations using molecular markers: A review

“…Helicanthus elastica (mango mistletoe) showed molecular differences in genomic DNA while growing on five different host trees when analyzed using four random RAPD primers [27]. RAPD was used to evaluate the genetic diversity among 20 Poincianella pyramidalis used in seed collection and conservation [28]. RAPD was also used together with intersimple sequence repeats (ISSRs) to study the genetic diversity of 18 Iranian populations of Nigella sativa, showing that these molecular markers can be useful for identifying and classifying plant species [29].…”

Forest inventory and the genetic diversity of the remaining fragment of Hymenaea courbaril L.

“…(Shahira et al, 2016); Conyza bonariensis (Silva;Agostinetto;Vargas, 2016) and Abelmoschus species (Pravin;Shrikant;Venkataraman, 2018). The existence of the most recent molecular marker techniques does not underestimate the RAPD utility as a tool for the development of strategies, for the conservation of individuals and their seed collections (Santana et al, 2008;Gois, 2014a;Belarmino et al, 2017). These molecular markers are often recommended in genome studies of little-known species like Himenaea courbaril.…”