Research Article Comparison Between Real-Time Polymerase Chain Reaction and DNA-Microarray in Detection and Identification of Mycobacterium Species

Gaber, Asaad; Hamed, Hzem; Elsawy, Essam

doi:10.4238/gmr16039837

Cited by 6 publications

(10 citation statements)

References 25 publications

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“…M. kansasii is the most common NTM detected among 3 immunocompromised patients (10%) and among the NTM (3/7 = 42.9%) in this study. Similarly, a previous study from Egypt reported M. kansasii (37.5%) as the most prevalent NTM [17]. M. kansasii was also previously reported in other countries such as Iran and Turkey as the third most common SGM in clinical isolates in the Middle East region [23].…”

Section: Discussionsupporting

confidence: 73%

“…In this study, this species was detected in an immunocompromised patient with bone marrow failure. Also, it was previously reported in Egypt [17]. Although it is rare and less virulent than other NTM, a recent study describes M. gordonae in a series of 7 immunocompetent and immunocompromised patients [28].…”

Section: Discussionmentioning

confidence: 97%

“…Over the last decade, several studies were conducted regarding the burden of M. tuberculosis, its genetic diversity, and drug resistance to antimycobacterial drugs in Egypt [ 12 , 13 , 14 , 15 ]. However, very few studies have reported the clinical existence of NTM in Egypt [ 16 , 17 ]. The resemblance of the clinical presentation of NTM to the well-established TB presents a tough challenge in diagnosis and treatment.…”

Section: Discussionmentioning

confidence: 99%

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Simultaneous Detection of Mycobacterium tuberculosis and Atypical Mycobacteria by DNA-Microarray in Egypt

et al. 2022

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Background and Objectives: Immunocompromised patients are considered a high-risk group to develop mycobacterial infections with either pulmonary and/or extra-pulmonary diseases. Low-cost and low-density (LCD) DNA-microarray is considered an easy and efficient method to detect typical and atypical Mycobacterial species. Materials and methods: Thirty immunocompromised patients were recruited to provide their clinical specimens (sputum, serum, urine and lymph node aspirate). Both Real-Time PCR and LCD-microarray techniques were performed and compared to the conventional methods of Ziehl-Neelsen (ZN) staining and Lowenstein Jensen (LJ) culturing. Results and conclusion: Mycobacterium tuberculosis complex (MTBC) was detected in all 30 clinical specimens (100% sensitivity) by Real-Time PCR and LCD-microarray. Additionally, co-infection with 4 atypical species belonging to mycobacteria other than tuberculosis (MOTT) was identified in 7 sputum specimens. These atypical mycobacterial species were presented as M. kansasii 10% (n=3), M. avium complex 6.6% (n=2), M. gordanae 3.3% (n=1) and M. peregnium 3.3% (n=1). This study documents the presence of certain species of atypical mycobacteria among immunocompromised patients in Egypt. To the best of our knowledge, this is the first detection of M. peregenium among clinical specimen in Egypt.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Simultaneous Detection of Mycobacterium tuberculosis and Atypical Mycobacteria by DNA-Microarray in Egypt

et al. 2022

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“…It is based on two polymerase chain reactions which are combined prior to hybridization into one array. Identifying non-tuberculous Mycobacterium depends on Primer mix A, which is directed against highly conserved motifs flanking the internal transcribed spacer region (ITS) of the 16SrRNA gene, whereas M. tuberculosis is identified by primer mix B, which is directed against the insertion element IS6110 [ 20 ]. All procedures were performed following the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Surgical site infections by atypical mycobacteria: prevalence and species characterization using MALDI-TOF and molecular LCD chip array

et al. 2022

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Background Surgical site infection (SSI) is a post-operative complication of high concern with adverse impact on patient prognosis and public health systems. Recently, SSI pathogens have experienced a change in microbial profile with increasing reports of non-tuberculous mycobacteria (NTM) as important pathogens. Aim of the study The study aimed to detect the prevalence of NTM among cases with SSIs and describe their species using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and PCR-based microarray. Methods The study was conducted with 192 pus samples collected from patients with SSI. Mycobacterial investigations were done in the form of Ziehl–Neelsen (ZN) smears for acid-fast bacilli, automated mycobacterial culture to isolate mycobacteria, followed by immunochromatography test to predict NTM. NTM-positive cultures were tested by MALDI -TOF MS and PCR-based microarray to reach species-level identification. Results Mycobacterial growth was found in 11/192 samples (5.7%) and identified as 4 NTM and 7 M. tuberculosis isolates with prevalence of 2.1% and 3.64%, respectively. The NTM species were described by MALDI-TOF as M. abscessus, M. porcinum, M. bacteremicum, and M. gordonae. Microarray agreed with MALDI-TOF in identifying one isolate (M. abscessus), while two isolates were classified as belonging to broad groups and one isolate failed to be identified. Conclusions The prevalence of NTM among SSI was found to be low, yet have to be considered in the diagnosis of mycobacteria. Employing advanced technologies in diagnosis is recommended to guide for appropriate treatment.

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“…In POC diagnosis, widely used urinalysis devices include dip strips or sticks for pregnancy testing, urinary tract infections (UTIs), and urine test strips for other types of analytes of urine samples, for example, Specific Gravity, Creatinine, Glucose, Ions, Ketones, Lactate, Nitrite, pH, Protein, and Uric Acid (Kingston et al 2003;Suresh et al 2018). For high throughput detection, DNA microarrays can be used for simultaneous detection of different DNA markers in urine (Gaber et al 2017). To avoid the inconvenience with urine sample storage and transportation, POC diagnosis devices, which can be easily implemented at the clinic or even at home, are highly desired in urine testing.…”

Section: Introductionmentioning

confidence: 99%

An immunoassay cassette with a handheld reader for HIV urine testing in point-of-care diagnostics

Yang

Gong

et al. 2020

Biomed Microdevices

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Currently, most HIV tests are performed with blood samples, or alternatively saliva samples are used for HIV testing. Simple HIV tests need to be performed in hospitals or other medical agencies instead of more invasive HIV blood tests. To enable point-ofcare (POC) HIV diagnostics, based on a recently developed lateral flow strip for HIV urine testing, a microfluidic immunoassay cassette with a handheld optical reader is developed. Based on lateral flow strip with gold colloid reporter, the integrated immunoassay cassette can perform sample introduction, metering, discharging, applying and detection which simplifies HIV testing. An indicator is incorporated into the cassette to guide sample introduction based on color change, and further, the excess test sample is stored inside the sealed cassette to avoid any contamination. The low-cost handheld optical reader can provide a test result within a few seconds, which is useful for simple, sensitive and affordable HIV onsite detection. Instead of using normal white LEDs, a customized back light module embedded with green LEDs is adopted to illuminate the lateral flow strip with an appropriate working current to achieve optimal performance. Compared to the standard lateral flow strips using a benchtop reader, with the disposable immunoassay cassette assisted by the handheld optical reader, more convenient, easier-to-operate, and more affordable HIV urine testing can be achieved in POC diagnostics.

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Research Article Comparison Between Real-Time Polymerase Chain Reaction and DNA-Microarray in Detection and Identification of Mycobacterium Species

Cited by 6 publications

References 25 publications

Simultaneous Detection of <b><i>Mycobacterium tuberculosis</i></b> and Atypical Mycobacteria by DNA-Microarray in Egypt

Simultaneous Detection of <b><i>Mycobacterium tuberculosis</i></b> and Atypical Mycobacteria by DNA-Microarray in Egypt

Surgical site infections by atypical mycobacteria: prevalence and species characterization using MALDI-TOF and molecular LCD chip array

An immunoassay cassette with a handheld reader for HIV urine testing in point-of-care diagnostics

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