Requirement of histone acetyltransferases HAM1 and HAM2 for epigenetic modification of FLC in regulating flowering in Arabidopsis

Xiao, Jun; Zhang, Hong; Xing, Lijing; Xu, Shujuan; Liu, Huanhuan; Chong, Kang; Xu, Yunyuan

doi:10.1016/j.jplph.2012.11.007

Cited by 73 publications

(74 citation statements)

References 43 publications

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“…When we compared the distribution patterns of these histone modifications, H3K36me3 and H4K5Ace levels were peaked at the exon 1, whilst H3Ace and H3K4me3 showed higher levels at the promoter (Figure 6D). Thus, we checked the possible interaction between MRG1/2 and MYST HATs, HAM1/2 proteins, that catalyse H4K5Ace (33,34). First, we carried out bimolecular fluorescence complementation (BiFC) assay by transiently co-expressing a translational fusion of MRG1/2 to the N-terminal portion of YFP (MRG1/2-nYFP) and fusion of HAM1/2 to the C-terminal portion of YFP (HAM1/2-cYFP) in tobacco.…”

Section: Resultsmentioning

confidence: 99%

“…In Arabidopsis , there are two functionally redundant MYST family proteins, namely, HISTONE ACETYLTRANSFERASE OF THE MYST FAMILY 1 (HAM1) and HAM2 (32). HAM1 and HAM2 have HAT activity specific to H4K5 in vitro and in vivo (33,34). The double-mutant ham1 ham2 is not viable, and mutation of either ham1 or ham2 did not show any defects in vegetative and reproductive stages (32).…”

Section: Introductionmentioning

confidence: 99%

“…The double-mutant ham1 ham2 is not viable, and mutation of either ham1 or ham2 did not show any defects in vegetative and reproductive stages (32). Interestingly, ham1/+ ham2/+ plants showed a reduction in the FLOWERING LOCUS C ( FLC ) expression level, with a reduction of H4K5 acetylation at the FLC locus (32,34), suggesting that HAM1 and HAM2 have essential functions in various developmental processes, including flowering control.…”

Section: Introductionmentioning

confidence: 99%

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Arabidopsis MRG domain proteins bridge two histone modifications to elevate expression of flowering genes

Gan

Zhou

et al. 2014

Nucleic Acids Research

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Trimethylation of lysine 36 of histone H3 (H3K36me3) is found to be associated with various transcription events. In Arabidopsis, the H3K36me3 level peaks in the first half of coding regions, which is in contrast to the 3′-end enrichment in animals. The MRG15 family proteins function as ‘reader’ proteins by binding to H3K36me3 to control alternative splicing or prevent spurious intragenic transcription in animals. Here, we demonstrate that two closely related Arabidopsis homologues (MRG1 and MRG2) are localised to the euchromatin and redundantly ensure the increased transcriptional levels of two flowering time genes with opposing functions, FLOWERING LOCUS C and FLOWERING LOCUS T (FT). MRG2 directly binds to the FT locus and elevates the expression in an H3K36me3-dependent manner. MRG1/2 binds to H3K36me3 with their chromodomain and interact with the histone H4-specific acetyltransferases (HAM1 and HAM2) to achieve a high expression level through active histone acetylation at the promoter and 5′ regions of target loci. Together, this study presents a mechanistic link between H3K36me3 and histone H4 acetylation. Our data also indicate that the biological functions of MRG1/2 have diversified from their animal homologues during evolution, yet they still maintain their conserved H3K36me3-binding molecular function.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Arabidopsis MRG domain proteins bridge two histone modifications to elevate expression of flowering genes

Gan

Zhou

et al. 2014

Nucleic Acids Research

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“…5, A and B). Beside HAM1/HAM2-mediated H4K5ac (Earley et al, 2007;Xiao et al, 2013), several other K-residues of histone H3 are also known as being subject to acetylation modification. We further extend our analysis to examine H3K9, H3K14, H3K27, and H3K36 acetylation in shade response.…”

Section: Pif7/mrg2-mediated Shade-induction Of Gene Expression Is Assmentioning

confidence: 99%

Linking PHYTOCHROME-INTERACTING FACTOR to Histone Modification in Plant Shade Avoidance

Peng

Zhou

et al. 2017

Plant Physiol.

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Shade avoidance syndrome (SAS) allows a plant grown in a densely populated environment to maximize opportunities to access to sunlight. Although it is well established that SAS is accompanied by gene expression changes, the underlying molecular mechanism needs to be elucidated. Here, we identify the H3K4me3/H3K36me3-binding proteins, Morf Related Gene (MRG) group proteins MRG1 and MRG2, as positive regulators of shade-induced hypocotyl elongation in Arabidopsis (Arabidopsis thaliana). MRG2 binds PHYTOCHROME-INTERACTING FACTOR7 (PIF7) and regulates the expression of several common downstream target genes, including YUCCA8 and IAA19 involved in the auxin biosynthesis or response pathway and PRE1 involved in brassinosteroid regulation of cell elongation. In response to shade, PIF7 and MRG2 are enriched at the promoter and gene-body regions and are necessary for increase of histone H4 and H3 acetylation to promote target gene expression. Our study uncovers a mechanism in which the shade-responsive factor PIF7 recruits MRG1/MRG2 that binds H3K4me3/H3K36me3 and brings histone-acetylases to induce histone acetylations to promote expression of shade responsive genes, providing thus a molecular mechanistic link coupling the environmental light to epigenetic modification in regulation of hypocotyl elongation in plant SAS.

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“…ChIP experiments were performed as described (Bowler et al, 2004;Xiao et al, 2013) using 2 g of young seedling tissue for each sample. Antibodies with specificity to H3K4me2, H3K4me3, H3K27me3 (Millipore), or H3K36me3 (Signalway Antibody) were used to immunoprecipitate the chromatin.…”

Section: Chip Assaymentioning

confidence: 99%

AtJAC1 Regulates Nuclear Accumulation of GRP7, Influencing RNA Processing of FLC Antisense Transcripts and Flowering Time in Arabidopsis

Xiao

et al. 2015

Plant Physiol.

Self Cite

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Lectins selectively recognize sugars or glycans for defense in living cells, but less is known about their roles in the development process and the functional network with other factors. Here, we show that Arabidopsis (Arabidopsis thaliana) JACALIN-LECTIN LIKE1 (AtJAC1) functions in flowering time control. Loss of function of AtJAC1 leads to precocious flowering, whereas overexpression of AtJAC1 causes delayed flowering. AtJAC1 influences flowering through regulation of the key flowering repressor gene FLOWERING LOCUS C (FLC). Genetic analysis revealed that AtJAC1's function is mostly dependent on GLYCINE-RICH RNA-BINDING PROTEIN7 (GRP7), an upstream regulator of FLC. Biochemical and cell biological data indicated that AtJAC1 interacted physically with GRP7 specifically in the cytoplasm. AtJAC1 influences the nucleocytoplasmic distribution of GRP7, with predominant nuclear localization of GRP7 when AtJAC1 function is lost but retention of GRP7 in the cytoplasm when AtJAC1 is overexpressed. A temporal inducible assay suggested that AtJAC1's regulation of flowering could be compromised by the nuclear accumulation of GRP7. In addition, GRP7 binds to the antisense precursor messenger RNA of FLC through a conserved RNA motif. Loss of GRP7 function leads to the elevation of total FLC antisense transcripts and reduced proximal-distal polyadenylation ratio, as well as histone methylation changes in the FLC gene body region and increased total functional sense FLC transcript. Attenuating the direct binding of GRP7 with competing artificial RNAs leads to changes of FLC antisense precursor messenger RNA processing and flowering transition. Taken together, our study indicates that AtJAC1 coordinates with GRP7 in shaping plant development through the regulation of RNA processing in Arabidopsis.

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Requirement of histone acetyltransferases HAM1 and HAM2 for epigenetic modification of FLC in regulating flowering in Arabidopsis

Cited by 73 publications

References 43 publications

Arabidopsis MRG domain proteins bridge two histone modifications to elevate expression of flowering genes

Arabidopsis MRG domain proteins bridge two histone modifications to elevate expression of flowering genes

Linking PHYTOCHROME-INTERACTING FACTOR to Histone Modification in Plant Shade Avoidance

AtJAC1 Regulates Nuclear Accumulation of GRP7, Influencing RNA Processing of FLC Antisense Transcripts and Flowering Time in Arabidopsis

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