Reproduction Multitasking: The Male Gametophyte

Hafidh, Said; Honys, David

doi:10.1146/annurev-arplant-080620-021907

Cited by 76 publications

(56 citation statements)

References 208 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The coordination of reprogramming events at the molecular/genetic level defines the integrity of ontogenic decisions ( Hafidh and Honys, 2021 ). Here, developmental decisions mediated by members of the three-amino-acid-loop-extension (TALE) class of homeoproteins characterized by a highly conserved DNA-binding homeodomain (HD), are prominent ( Bowman et al, 2016 ).…”

Section: Alternation Of Generation – Definition and Common Themesmentioning

confidence: 99%

“…The main functions of meiosis are the reduction of chromosome numbers and recombination which provides genetic variability. However, it also represents a critical stage for the ontogeny switch, representing the sporophyte-to-gametophyte transition ( Hafidh and Honys, 2021 ). Our efforts to understand meiotic gene functions during the transition from the sporophyte cell lineage (the pollen mother cell) to the gametophyte cell lineage (microspore) are still in their infancy.…”

Section: Alternation Of Generation In Angiospermsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Control of Sporophyte-Gametophyte Ontogeny and Transition in Plants

et al. 2022

View full text Add to dashboard Cite

Alternation of generations between a sporophytic and gametophytic developmental stage is a feature common to all land plants. This review will discuss the evolutionary origins of these two developmental programs from unicellular eukaryotic progenitors establishing the ability to switch between haploid and diploid states. We will compare the various genetic factors that regulate this switch and highlight the mechanisms which are involved in maintaining the separation of sporophytic and gametophytic developmental programs. While haploid and diploid stages were morphologically similar at early evolutionary stages, largely different gametophyte and sporophyte developments prevail in land plants and finally allowed the development of pollen as the male gametes with specialized structures providing desiccation tolerance and allowing long-distance dispersal. Moreover, plant gametes can be reprogrammed to execute the sporophytic development prior to the formation of the diploid stage achieved with the fusion of gametes and thus initially maintain the haploid stage. Upon diploidization, doubled haploids can be generated which accelerate modern plant breeding as homozygous plants are obtained within one generation. Thus, knowledge of the major signaling pathways governing this dual ontogeny in land plants is not only required for basic research but also for biotechnological applications to develop novel breeding methods accelerating trait development.

show abstract

Section: Alternation Of Generation – Definition and Common Themesmentioning

confidence: 99%

Section: Alternation Of Generation In Angiospermsmentioning

confidence: 99%

Molecular Control of Sporophyte-Gametophyte Ontogeny and Transition in Plants

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Pollen developed as highly synchronous generative tissue producing sperm cells and plays a specialized role in double fertilization in angiosperms [1]. Bicellular pollen, for example in Nicotiana sp.…”

Section: Introductionmentioning

confidence: 99%

Transformation of Seed Non-Transmissible Hop Viroids in Nicotiana benthamiana Causes Distortions in Male Gametophyte Development

Steinbachová

Matoušek

Steger

et al. 2021

Plants

Self Cite

View full text Add to dashboard Cite

Viroids are small, non-coding, parasitic RNAs that promote developmental distortions in sensitive plants. We analyzed pollen of Nicotiana benthamiana after infection and/or ectopic transformation with cDNAs of citrus bark cracking viroid (CBCVd), apple fruit crinkle viroid (AFCVd) and potato spindle tuber viroid (PSTVd) variant AS1. These viroids were seed non-transmissible in N. benthamiana. All viroids propagated to high levels in immature anthers similar to leaves, while their levels were drastically reduced by approximately 3.6 × 103, 800 and 59 times in mature pollen of CBCVd, AFCVd and PSTVd infected N. benthamiana, respectively, in comparison to leaves. These results suggest similar elimination processes during male gametophyte development as in the Nicotiana tabacum we presented in our previous study. Mature pollen of N. benthamiana showed no apparent defects in infected plants although all three viroids induced strong pathological symptoms on leaves. While Nicotiana species have naturally bicellular mature pollen, we noted a rare occurrence of mature pollen with three nuclei in CBCVd-infected N. benthamiana. Changes in the expression of ribosomal marker proteins in AFCVd-infected pollen were detected, suggesting some changes in pollen metabolism. N. benthamiana transformed with 35S-driven viroid cDNAs showed strong symptoms including defects in pollen development. A large number of aborted pollen (34% and 62%) and a slight increase of young pollen grains (8% and 15%) were found in mature pollen of AFCVd and CBCVd transformants, respectively, in comparison to control plants (3.9% aborted pollen and 0.3% young pollen). Moreover, pollen grains with malformed nuclei or trinuclear pollen were found in CBCVd-transformed plants. Our results suggest that “forcing” overexpression of seed non-transmissible viroid led to strong pollen pathogenesis. Viroid adaptation to pollen metabolism can be assumed as an important factor for viroid transmissibility through pollen and seeds.

show abstract

“…The development of male gametophyte in vascular plants is a complex process that requires coordinated action and participation of numerous cells and both sporophyte and gametophyte tissues. The life cycle of the male gametophyte can be divided into a developmental phase leading to the formation of mature pollen grains and a functional (progamic) phase beginning with the impingement of pollen on the stigma surface and ending with double fertilization (Hafidh and Honys, 2021).…”

Section: Introductionmentioning

confidence: 99%

Hormonome Dynamics During Microgametogenesis in Different Nicotiana Species

et al. 2021

Self Cite

View full text Add to dashboard Cite

Plant microgametogenesis involves stages leading to the progressive development of unicellular microspores into mature pollen. Despite the active and continuing interest in the study of male reproductive development, little is still known about the hormonomics at each ontogenetic stage. In this work, we characterized the profiles and dynamics of phytohormones during the process of microgametogenesis in four Nicotiana species (Nicotiana tabacum, Nicotiana alata, Nicotiana langsdorffii, and Nicotiana mutabilis). Taking advantage of advanced HPLC-ESI-MS/MS, twenty to thirty endogenous hormone derivatives were identified throughout pollen ontogenesis, including cytokinins, auxins, ABA and its derivatives, jasmonates, and phenolic compounds. The spectra of endogenous phytohormones changed dynamically during tobacco pollen ontogeny, indicating their important role in pollen growth and development. The different dynamics in the accumulation of endogenous phytohormones during pollen ontogenesis between N. tabacum (section Nicotiana) and the other three species (section Alatae) reflects their different phylogenetic positions and origin within the genus Nicotiana. We demonstrated the involvement of certain phytohormone forms, such as cis-zeatin- and methylthiol-type CKs, some derivatives of abscisic acid, phenylacetic and benzoic acids, in pollen development for the first time here. Our results suggest that unequal levels of endogenous hormones and the presence of specific derivatives may be characteristic for pollen development in different phylogenetic plant groups. These results represent the currently most comprehensive study of plant hormones during the process of pollen development.

show abstract

Reproduction Multitasking: The Male Gametophyte

Cited by 76 publications

References 208 publications

Molecular Control of Sporophyte-Gametophyte Ontogeny and Transition in Plants

Molecular Control of Sporophyte-Gametophyte Ontogeny and Transition in Plants

Transformation of Seed Non-Transmissible Hop Viroids in Nicotiana benthamiana Causes Distortions in Male Gametophyte Development

Hormonome Dynamics During Microgametogenesis in Different Nicotiana Species

Contact Info

Product

Resources

About