Reproducing Human Brain Development In Vitro: Generating Cerebellar Neurons for Modelling Cerebellar Ataxias

Bekman, Evguenia; Silva, Teresa P; Cotovio, João P.; Almeida, Rita Mendes de

doi:10.1007/978-3-030-43939-2_11

Cited by 1 publication

(1 citation statement)

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“…This method recapitulates anteroposterior and dorsoventral patterning and thereby induces MATH1 -expressing mitotic neural progenitors, which can later be differentiated into cerebellar granule cells. Purkinje cells derived from hiPSCs initially had an immature phenotype, and thus needed to be co-cultured with mouse cerebellar granule cell precursors to allow for maturation[ 129 , 140 ]. However, cells made using this approach had substantial functional variability.…”

Section: Brain Organoid Development From Hpscsmentioning

confidence: 99%

Neural lineage differentiation of human pluripotent stem cells: Advances in disease modeling

Yan¹,

Qian²,

Woodard³

et al. 2023

World J Stem Cells

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Brain diseases affect 1 in 6 people worldwide. These diseases range from acute neurological conditions such as stroke to chronic neurodegenerative disorders such as Alzheimer’s disease. Recent advancements in tissue-engineered brain disease models have overcome many of the different shortcomings associated with the various animal models, tissue culture models, and epidemiologic patient data that are commonly used to study brain disease. One innovative method by which to model human neurological disease is via the directed differentiation of human pluripotent stem cells (hPSCs) to neural lineages including neurons, astrocytes, and oligodendrocytes. Three-dimensional models such as brain organoids have also been derived from hPSCs, offering more physiological relevance due to their incorporation of various cell types. As such, brain organoids can better model the pathophysiology of neural diseases observed in patients. In this review, we will emphasize recent developments in hPSC-based tissue culture models of neurological disorders and how they are being used to create neural disease models.

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Section: Brain Organoid Development From Hpscsmentioning

confidence: 99%