Reproducibility of single-pass donor DSAEK tissue preparation with the MORIA single-use microkeratome

Saunier, Valentine; Robinet-Perrin, Antoine; Costet, C; Touboul, D

doi:10.1016/j.jfo.2016.06.004

Cited by 6 publications

(1 citation statement)

References 19 publications

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“…The Moria microkeratome has been used and studied in different articles reporting lenticule thicknesses of 63 ± 29 µm at 5 months, [4] 69.9 ± 20.8 µm at 3 months [9] and 89.4 ± 26.2 µm at 6 months. [25] However, most of these studies used the double-pass microkeratome technique,[8, 25,26] which involves signi cant risk for perforation, endothelial cell loss and stromal surface irregularity. Other studies have used a single 350 µm blade head in 10 corneas, [9] a combination of femtosecond [10] or a manual rotation system.…”

Section: Discussionmentioning

confidence: 99%

Nomogram for Single-pass Two-speed Automated Microkeratome Graft Preparation for Ultrathin Descemet Stripping Automated Endothelial Keratoplasty

Sánchez-Ventosa

Cano-Ortiz²,

L.³

et al. 2021

Preprint

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PurposeTo create a nomogram including the translational speed of the microkeratome blade, microkeratome head size and precut tissue thickness to predict the postcut thickness for Descemet stripping automated endothelial keratoplasty (DSAEK) to obtain the thinnest possible graft.MethodsThis prospective study incorporated 48 grafts for DSAEK from March 2017 to June 2020. Corneal tissue for DSAEK was prepared by 3 experienced physicians using the Moria Evolution 3E (Moria Inc, Antony, France) microkeratome with 400, 450 and 500 mm head sizes. Precut central corneal thickness was measured with a DGH 550 handheld pachymeter (Pachette 2), taking an average of 3 readings. The microkeratome head was selected according to precut tissue thickness. The target donor lamella thickness ranged from 70 to 120 μm, and the selected microkeratome head size was 150μm less than the donor cornea thickness. Two translational speeds were used for the microkeratome cuts. One month after surgery, the central lenticular thickness was measured with a Visante® Optical Coherence Tomography caliper (Carl Zeiss Meditec Inc, Germany). A descriptive analysis was performed.ResultsForty-eight donor grafts were prepared. Mean graft thickness was 97.58 ± 29.84 μm (range 39-176 μm). Of the 48 samples, central graft thickness was <120µm (81.3%) in 39, <100 µm (58.3%) in 28 and <80µm (37.5%) in 18 at 1-month follow-up. There were no statically significant differences between translational speeds. ConclusionsA nomogram with an automated microkeratome to obtain thin grafts for DSAEK provided good graft thickness results without donor waste.

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