Reporting Data on Auditory Brainstem Responses (ABR) in Rats: Recommendations Based on Review of Experimental Protocols and Literature

Domarecka, Ewa; Kalcıoğlu, M. Tayyar; Mutlu, Ahmet; Özgür, Abdulkadir; Smit, Jasper V.; Szczepek, Agnieszka J.

doi:10.3390/brainsci11121596

Cited by 9 publications

(13 citation statements)

References 77 publications

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“…Even within a single species, the need for standardized and consistent ABR recording is critical. 31 …”

Section: Resultsmentioning

confidence: 99%

Revisiting the Chicken Auditory Brainstem Response: Frequency Specificity, Threshold Sensitivity, and Cross Species Comparison

Ordiway,

McDonnell,

Sanchez

2024

J Exp Neurosci

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The auditory brainstem response (ABR) is important for both clinical and basic auditory research. It is a non-invasive measure of hearing function with millisecond-level precision. The ABR can not only measure the synchrony, speed, and efficacy of auditory physiology but also detect different modalities of hearing pathology and hearing loss. ABRs are easily acquired in vertebrate animal models like reptiles, birds, and mammals, and complement existing molecular, developmental, and systems-level research. One such model system is the chicken; an excellent animal for studying auditory development, structure, and function. However, the ABR for chickens was last reported nearly 4 decades ago. The current study examines how decades of ABR characterization in other animal species support findings from the chicken ABR. We replicated and expanded on previous research using 43 chicken hatchlings 1- and 2-day post-hatch. We report that click-evoked chicken ABRs presented with a peak waveform morphology, amplitude, and latency like previous avian studies. Tone-evoked ABRs were found for frequencies from 250 to 4000 Hertz (Hz) and exhibited a range of best sensitivity between 750 and 2000 Hz. Objective click-evoked and tone-evoked ABR thresholds were comparable to subjective thresholds. With these revisited measurements, the chicken ABR still proves to be an excellent example of precocious avian development that complements decades of molecular, neuronal, and systems-level research in the same model organism.

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“…Even within a single species, the need for standardized and consistent ABR recording is critical. 31 …”

Section: Resultsmentioning

confidence: 99%

Revisiting the Chicken Auditory Brainstem Response: Frequency Specificity, Threshold Sensitivity, and Cross Species Comparison

Ordiway,

McDonnell,

Sanchez

2024

J Exp Neurosci

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“…Since the ABR signal typically occurs in the 1-2.5 microVolt range, removing cables, wires, and noise generators from the test area is crucial. The impact of the animal fat layer in the head/neck region on impedance has been previously discussed [24]. Since the ABR signal typically occurs in the 1-2.5 microVolt range, removing cables, wires, and noise generators from the test area is crucial.…”

Section: Performing Abr: Equipmentmentioning

confidence: 99%

“…each of these domains on the results of the ABR [24,25]. Here, we synthesized the knowledge on performing ABR in experimental animals to provide general recommendations.…”

Section: Introductionmentioning

confidence: 99%

Universal Recommendations on Planning and Performing the Auditory Brainstem Responses (ABR) with a Focus on Mice and Rats

Domarecka

Szczepek

2023

Audiology Research

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Translational audiology research aims to transfer basic research findings into practical clinical applications. While animal studies provide essential knowledge for translational research, there is an urgent need to improve the reproducibility of data derived from these studies. Sources of variability in animal research can be grouped into three areas: animal, equipment, and experimental. To increase standardization in animal research, we developed universal recommendations for designing and conducting studies using a standard audiological method: auditory brainstem response (ABR). The recommendations are domain-specific and are intended to guide the reader through the issues that are important when applying for ABR approval, preparing for, and conducting ABR experiments. Better experimental standardization, which is the goal of these guidelines, is expected to improve the understanding and interpretation of results, reduce the number of animals used in preclinical studies, and improve the translation of knowledge to the clinic.

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“…This comparative analysis is promising, but care must be taken when comparing ABR results with different experimental parameters. Even within a single species, the need for standardized and consistent ABR recording is critical (Domarecka et al, 2021).…”

Section: Abr Latency Between Seven Different Speciesmentioning

confidence: 99%

The Auditory Brainstem Response of the Chicken Hatchling

Ordiway¹,

McDonnell²,

Sanchez³

2022

Preprint

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The auditory brainstem response (ABR) is a useful approach for evaluating hearing function. It is easily acquired in vertebrate animal models like reptiles, birds, and mammals, and routinely utilized in clinical audiology with human patients. The ABR is complementary in animal models with existing developmental and in vitro research. However, despite the domestic white leghorn chicken (gallus gallus domesticus) being studied extensively at the molecular, cellular, and system levels for decades, there exists no modern characterization of the chicken hatchling ABR. To address this gap, we used click- and tone-burst stimuli to elicit the ABR in 43 wild type chicken hatchlings one- and two-days post hatch (P1 and P2). Click-evoked ABRs presented with three to four positive going microvolt (µV) peaks within 6 milliseconds (ms) post stimulus onset. Tone-evoked ABRs resembled findings in other avian species; thresholds from 100–4000 Hertz (Hz) exhibited a range of best sensitivity between 750–2000 Hz. Population latency/intensity functions revealed an increase in peak latency of roughly 300 microseconds (µs) per a decrease of 20 decibels sound pressure level (dB SPL) for both click- and tone-evoked ABRs. The chicken hatchling ABR not only complements existing molecular, cellular, and systems level research, but also resembles the ABR waveform of altricial avian species, highlighting the precocious development of the chicken.

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Reporting Data on Auditory Brainstem Responses (ABR) in Rats: Recommendations Based on Review of Experimental Protocols and Literature

Cited by 9 publications

References 77 publications

Revisiting the Chicken Auditory Brainstem Response: Frequency Specificity, Threshold Sensitivity, and Cross Species Comparison

Revisiting the Chicken Auditory Brainstem Response: Frequency Specificity, Threshold Sensitivity, and Cross Species Comparison

Universal Recommendations on Planning and Performing the Auditory Brainstem Responses (ABR) with a Focus on Mice and Rats

The Auditory Brainstem Response of the Chicken Hatchling

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