Replicative capacity of four porcine enteric coronaviruses in LLC-PK1 cells

Xiao, Wenwen; Wang, Xunlei; Jing, Wang; Fang, Puxian; Xiao, Shaobo; Fang, Liurong

doi:10.1007/s00705-020-04947-2

Cited by 8 publications

(4 citation statements)

References 25 publications

(31 reference statements)

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“…Currently, PEDV has been reported to mainly infect porcine cells such as IEC(11), IPEC-J2 ( 35 , 36 ), and LLC-PK1 ( 37 ). However, we found that the infection of PEDV to LLC-PK1 cells was strain-specific.…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanism of Porcine Epidemic Diarrhea Virus Cell Tropism

Dong

et al. 2022

mBio

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Section: Discussionmentioning

confidence: 99%

Molecular Mechanism of Porcine Epidemic Diarrhea Virus Cell Tropism

Dong

et al. 2022

mBio

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“…It is similar by structure but very distinct in the type of target cell from the porcine respiratory coronavirus (PRCV), which aff ects the respiratory system. Th e latter has been shown to arise from TGEV by deletion of part of the viral protein S, resulting in a change in the tropism of the virus from the gastrointestinal tract to the respiratory tract [41].…”

Section: Fractionation and Affi Nity Chromatography Th Is Work Used T...mentioning

confidence: 99%

Both Maternal and Newborn IgMs Inhibit Transmissible Gastroenteritis Virus Internalization in LLC-PK1 Cells

et al. 2023

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Immunoglobulins M (IgMs) are the evolutionally oldest class of antibodies in higher eukaryotes. This pool of antibodies is one of the first to appear in humans and begins to be-synthesized at the early stages of the neonatal period. Most of the repertoire of IgMs of the newborns consists of the so-called «natural», or «naive», antibodies synthesized by the body without external antigenic stimulation. In addition to the classical functions of human immunoglobulins M (such as antigen recognition and initiation of innate immune responses), antibodies of this class exhibit a variety of non-canonical functions. The non-canonical functions are the action of antibodies as agonists/antagonists of various receptors, cleavage of antigen due to the catalytic activity of IgM, direct inactivation of pathogens in the absence of effector cells and molecules, etc. The aim of this work was to study and compare the antiviral activity of total preparations of immunoglobulin M of newborns and adults, obtained from umbilical cord and venous blood sera, on the LLC-PK1 cell line model infected with the transmissible gastroenteritis virus (TGEV). In addition, in the course of the studies, a decision was made to investigate the effect of combined preparations of immunoglobulin M on the change in signal transduction in the epidermal growth factor receptor as one of the mechanisms of TGEV internalization during infection of target cells. Methods. Highly purified preparations of total IgM of adults or newborns were obtained using the methodologies of sequential salt fractionation and affinity chromatography. This work used the model of the interaction of the transmissible gastroenteritis virus with cells of the LLC-PK1 line and the monitoring of changes in the phosphorylation state of the epidermal growth factor receptor of these cells during virus infection to study the effect of human IgM on the internalization of the virus and its interaction with the receptor system of the host cell. The degree of cytopathogenic effect of the virus was determined visually by changes in cell morphology. The mean infectious dose for transmissible gastroenteritis virus in the cell culture of the LLC-PK1 line was determined by the Reed-Muench method. Analysis of changes in the phosphorylation of the epidermal growth factor receptor was performed using the Western-blot analysis method. Results. The addition of a total high-purified sample of human IgM reduces the degree of efficiency of TGEV infection of the LLC-PK1 cell line and modulates the phosphorylation levels of these cells. Conclusions. The total preparations of IgM obtained from human venous blood of adults and from umbilical cord blood of newborns can affect the internalization of the transmissible gastroenteritis virus in the LLC-PK1 cell line. The original model of virus (TGEV) — cell line (LLC-PK1) was applied and tested to study the effect of native total preparations of immunoglobulin M on the internalization of the virus into the cell. The obtained data can be useful in further studies for a better understanding of the process of development and functioning of the immune system of newborns.

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“…The swine enteric coronaviruses offer a more suitable model for investigating coronaviral coinfections and recombination than coronaviruses that cause respiratory diseases. Different swine enteric coronaviruses can infect the same cells, providing the temporal and spatial conditions for genetic recombination [ 27 ]. These viruses also show tropism for the jejunum and ileum of piglets, providing favorable conditions for recombination during natural infections [ 4 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Coinfection and nonrandom recombination drive the evolution of swine enteric coronaviruses

Guo,

Lai,

Yang

et al. 2024

Emerging Microbes & Infections

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Coinfection with multiple viruses is a common phenomenon in clinical settings and is a crucial driver of viral evolution. Although numerous studies have demonstrated viral recombination arising from coinfections of different strains of a specific species, the role of coinfections of different species or genera during viral evolution is rarely investigated. Here, we analyzed coinfections of and recombination events between four different swine enteric coronaviruses that infect the jejunum and ileum in pigs, including porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and swine acute diarrhea syndrome coronavirus (SADS-CoV), and a deltacoronavirus, porcine deltacoronavirus (PDCoV). Various coinfection patterns were observed in 4,468 fecal and intestinal tissue samples collected from pigs in a 4-year survey. PEDV/PDCoV was the most frequent coinfection. However, recombination analyses have only detected events involving PEDV/TGEV and SADS-CoV/TGEV, indicating that inter-species recombination among coronaviruses is most likely to occur within the same genus. We also analyzed recombination events within the newly identified genus Deltacoronavirus and found that sparrows have played a unique host role in the recombination history of the deltacoronaviruses. The emerging virus PDCoV, which can infect humans, has a different recombination history. In summary, our study demonstrates that swine enteric coronaviruses are a valuable model for investigating the relationship between viral coinfection and recombination, which provide new insights into both inter- and intraspecies recombination events among swine enteric coronaviruses, and extend our understanding of the relationship between coronavirus coinfection and recombination.

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Replicative capacity of four porcine enteric coronaviruses in LLC-PK1 cells

Cited by 8 publications

References 25 publications

Molecular Mechanism of Porcine Epidemic Diarrhea Virus Cell Tropism

Molecular Mechanism of Porcine Epidemic Diarrhea Virus Cell Tropism

Both Maternal and Newborn IgMs Inhibit Transmissible Gastroenteritis Virus Internalization in LLC-PK1 Cells

Coinfection and nonrandom recombination drive the evolution of swine enteric coronaviruses

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