Replication of Polymer‐Based Peptide Microarrays by Multi‐Step Transfer

Abstract

Peptide microarrays serve as a high-throughput tool for the readout of protein-peptide interactions. We present a multi-step purification of high-complexity peptide microarrays by transfer to specialized surfaces. Only correctly synthesized peptides are transferred from the synthesis surface to various substrates where they can be immobilized by a variety of different anchor molecules. Here, we demonstrate the transfer to rigid streptavidin, epoxide, gold, and azide surfaces. The reported method reduces the cost of purified peptide array production through the reproduction of one array several times

A Trifunctional Linker for Purified 3D Assembled Peptide Structure Arrays

“…Assembled Peptide Structure on Surface via Transfer (Structured Synthesis) : In contrast to the unstructured synthesis, the 10:90‐PEGMA‐co‐MMA surface was prestructured via cLIFT to generate an array pattern. Therefore, Fmoc‐Gly‐OPfp was transferred and then coupled to the surface in the oven.…”

“…For the transfer, a custom‐built machine was used which presses a surface, containing cleaved peptides, onto a second array surface. The reaction medium between the two surfaces allows the diffusion of the cleaved peptides, which couple to the second surface without loss of lateral resolution.…”

Vertical Scanning Interferometry for Label-Free Detection of Peptide-Antibody Interactions

“…However, direct application of the known label-free detection methods, such as surface plasmon resonance methods [4,5,6], reflectometric interference spectroscopy [7], or resonator-like microstructures [8], is not possible for the surfaces on which commercially available high-density peptide arrays are synthesized. The transfer of the peptide spots to the standard free-label detection surfaces from a synthesis surface seems to be a very complicated procedure due to the spreading of the peptides cleaved and their amino-acid-dependent diffusion and adsorption [9].…”