Replication of influenza virus in a continuous cell line: High yield of infective virus from cells inoculated at high multiplicity

Choppin, Purnell W.

doi:10.1016/0042-6822(69)90354-7

Cited by 218 publications

(82 citation statements)

References 11 publications

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“…Thus, spin-label studies on synthetic bilayers, erythrocyte membranes, and influenza virus yield substantially the same conclusion: that the rotation of 1(12,3) is far more hindered by its environment than is the rotation of 1 (1,14). This relationship suggests that the lipid of the virus is organized into a bilayer.…”

Section: Resultssupporting

confidence: 50%

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Spin-Label Electron Spin Resonance Study of the Lipid-Containing Membrane of Influenza Virus

Landsberger

Lenard

Paxton

et al. 1971

Proc. Natl. Acad. Sci. U.S.A.

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The organization of the lipid-containing membrane of influenza virus has been studied by the use of three different lipid spin labels, and the results are compared with a parallel study on human erythrocyte ghosts. The lipid phase of the viral membrane is slightly more rigid than that of the erythrocyte ghosts. The data suggest that the viral lipid is arranged in a bilayer. The glycoprotein spikes covering the viral membrane were specifically removed by proteases, and no alteration in the environment of any of the three spin labels was detected. This suggests that the spikes are not involved in determining the organization of the lipid bilayer.

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Section: Resultssupporting

confidence: 50%

“…The splitting between the extreme high-and low-field peaks (A in Fig. 3 and Table 1) is greater in the virus than in the erythrocyte for both 1(12,3) and 1 (1,14). This indicates that these spin labels are located in a more immobilizing (i.e., more "rigid") environment in the virus particles than in the erythrocyte ghosts.…”

Section: Resultsmentioning

confidence: 77%

Spin-Label Electron Spin Resonance Study of the Lipid-Containing Membrane of Influenza Virus

Landsberger

Lenard

Paxton

et al. 1971

Proc. Natl. Acad. Sci. U.S.A.

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“…All cells were grown at 37"C in an atmosphere of 5% CO2 in air. Stocks of influenza virus, A/WSN(H~N~) strain, were prepared in MDBK cells (7), and stocks of the Indiana strain of VSV were propagated in BHK21 cells (32). Infectivity titers of influenza virus were assayed by plaquing on MDCK cells (42) and of VSV by plaque assay on BHK21 cells (32).…”

Section: Cells and Viruses: Mdck And Madin-darby Bovine Kidney (Mdbk)mentioning

confidence: 99%

Modulation of glycosylation and transport of viral membrane glycoproteins by a sodium ionophore.

Alonso-Caplen¹,

Compans²

1983

The Journal of Cell Biology

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Analysis of viral glycoprotein expression on surfaces of monensin-treated cells using a fluorescence-activated cell sorter (FACS) demonstrated that the sodium ionophore completely inhibited the appearance of the vesicular stomatitis virus (VSV) G protein on (Madin-Darby canine kidney) MDCK cell surfaces. In contrast, the expression of the influenza virus hemagglutinin (HA) glycoprotein on the surfaces of MDCK cells was observed to occur at high levels, and the time course of its appearance was not altered by the ionophore. Viral protein synthesis was not inhibited by monensin in either VSV-or influenza virus-infected cells. However, the electrophoretic mobilities of viral glycoproteins were altered, and analysis of pronase-derived glycopeptides by gel filtration indicated that the addition of sialic acid residues to the VSV G protein was impaired in monensin-treated cells. Reduced incorporation of fucose and galactose into influenza virus HA was observed in the presence of the ionophore, but the incompletely processed HA protein was cleaved, transported to the cell surface, and incorporated into budding virus particles. In contrast to the differential effects of monensin on VSV and influenza virus replication previously observed in monolayer cultures of MDCK cells, yields of both viruses were found to be significantly reduced by high concentrations of monensin in suspension cultures, indicating that cellular architecture may play a role in determining the sensitivity of virus replication to the drug. Nigericin, an ionophore that facilitates transport of potassium ions across membranes, blocked the replication of both influenza virus and VSV in MDCK cell monolayers, indicating that the ion specificity of ionophores influences their effect on the replication of enveloped viruses.Cells infected with enveloped RNA viruses provide valuable systems for elucidating the pathways involved in subcellular transport of membrane glycoproteins (8,20,44). As a result of viral inhibition of host protein synthesis, only one or a few viral membrane glycoproteins are synthesized in infected cells. These viruses possess limited genetic capacity, and the glycosylation and transport of their membrane glycoproteins to the cell surface are probably carried out by the same systems used by the host cell for biogenesis of its own membrane glycoproteins.Monovalent ionophores such as monensin, which is reported to interfere with the translocation of secretory as well as most membrane glycoproteins, have been used to characterize the pathways of intracellular glycoprotein transport in eucaryotic cells (18,(37)(38)(39)(40)(41)43). In addition, the effects of monensin on IgM and H2 glycosylation in lymphoid cells and fibronectin glycosylation in cultured human fibroblasts, as well as its influence on oligosaccharide maturation of

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“…The generation of DI Semliki Forest virus is also strongly influenced by the host cell (Stark & Kennedy, 1978) and the production of non-infectious influenza virus is similarly affected (Choppin, 1969). However, analysis of viral RNA in the latter study (Choppin & Pons, 1970) was incomplete since suitable techniques were not then available.…”

Section: Printed In Great Britainmentioning

confidence: 99%

Influence of the Host Cell on the Genomic and Subgenomic RNA Content of Defective-interfering Influenza Virus

Crumpton

Avery

Dimmock

1981

Journal of General Virology

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Replication of influenza virus in a continuous cell line: High yield of infective virus from cells inoculated at high multiplicity

Cited by 218 publications

References 11 publications

Spin-Label Electron Spin Resonance Study of the Lipid-Containing Membrane of Influenza Virus

Spin-Label Electron Spin Resonance Study of the Lipid-Containing Membrane of Influenza Virus

Modulation of glycosylation and transport of viral membrane glycoproteins by a sodium ionophore.

Influence of the Host Cell on the Genomic and Subgenomic RNA Content of Defective-interfering Influenza Virus

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