2014
DOI: 10.1371/journal.pone.0114614 View full text |Buy / Rent full text
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Abstract: This study describes an attempt to replicate experiments from the paper “Effect of BMAP-28 Antimicrobial Peptides on Leishmania major Promastigote and Amastigote Growth: Role of Leishmanolysin in Parasite Survival,” which was submitted to the Reproducibility Initiative for independent validation. The cathelicidin bovine myeloid antimicrobial peptide 28 (BMAP-28) and its isomers were previously shown to have potent antiparasitic activity against Leishmania major. We tested the effectiveness of L-BMAP-28 and two… Show more

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“…For example, in a recent replication effort of the anti-Leishmania activity of tested peptides, it was difficult to tell whether replication had been achieved or not; the peptides were found to have anti-Leishmania activity, but at concentrations 10 to 50 higher than in the original experiments and close to the toxicity range of eukaryotic human cells (18). Rejection of the null hypothesis in the two sets of experiments was insufficient to garner consensus about results reproducibility when consensus was missing about the operational scientific question, that is, whether the peptides had activity at low (and clinically relevant) con- Purposes to which findings will be put and consequences of false conclusions centrations or at any concentration.…”
Section: Results Reproducibilitymentioning
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“…For example, in a recent replication effort of the anti-Leishmania activity of tested peptides, it was difficult to tell whether replication had been achieved or not; the peptides were found to have anti-Leishmania activity, but at concentrations 10 to 50 higher than in the original experiments and close to the toxicity range of eukaryotic human cells (18). Rejection of the null hypothesis in the two sets of experiments was insufficient to garner consensus about results reproducibility when consensus was missing about the operational scientific question, that is, whether the peptides had activity at low (and clinically relevant) con- Purposes to which findings will be put and consequences of false conclusions centrations or at any concentration.…”
Section: Results Reproducibilitymentioning
“…In order to combat the resistant strains, various combinatorial therapies have been used. Due to increased drug selectivity, reduced toxicity and compatibility with the target protein structures AMPs, for example, dermaseptins, cecropins, melettin, etc., have been described as a first line of defense against pathogen invasion, including protozoa (Hopkins and Groom, 2002;Lynn et al, 2011;Mangoni et al, 2005;Marr et al, 2016;Toro et al, 2013;Ruiz-Santaquiteria et al, 2017) and are under intense investigation to improve their druggability. Their mode of action involves the perturbation of protozoan homeostasis by directly disrupting the cellular membranes, interaction and interference with key processes in the parasite metabolism (Afacan et al, 2012;Jenssen et al, 2006;Torrent et al, 2012b).…”
Section: Antiparasiticmentioning
“…The benefits of this trend are multifaceted, ranging from a minimization in the use of cell lines up to helping the improved planning of all biological assays, with the aim to maximize resources and minimize replication [ 2 ]. A key crossing issue is the recent global reproducibility initiative, which has targeted the replication of selected published experiments in specific bio-related areas to further share the benefits with the scientific community and which aims to advance scientific progress while reducing the replication of work [ 3 , 4 ]. The present study is designed to make a contribution to the above-mentioned prospects, focusing on a comprehensive assessment of the mathematical models currently in use for handling and interpreting in vitro experimental data.…”
Section: Introductionmentioning