Replicating infant-specific reactive astrocyte functions in the injured adult brain

Teo, Leon; Boghdadi, Anthony G.; Homman‐Ludiye, Jihane; Mundinano, Inaki‐Carril; Kwan, William C.; Bourne, James A.

doi:10.1016/j.pneurobio.2021.102108

Cited by 2 publications

(2 citation statements)

References 87 publications

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“…Induction of focal stroke to the marmoset primary visual cortex (V1) was performed by vasoconstrictor-mediated vascular occlusion of the calcarine branch of the posterior cerebral artery (PCAca), as detailed previously. 72,73 Briefly, following anesthesia (Alfaxalone 5mg/kg; maintained with inspired isoflurane 0.5-4%), a craniotomy and dural thinning was performed, followed by intracortical injections of endothelin-1 (ET-1; 0.1µl/30s pulse at 30s intervals, totaling ∼0.7 µL over 7 sites) proximal to the PCAca, which supplies operculum V1. The craniotomy was replaced, secured with tissue adhesive (Vetbond; 3M) and the skin sutured closed.…”

Section: Methodsmentioning

confidence: 99%

“…Brains were dissected, post-fixed and cryoprotected, as outlined previously. 72,73,76 Following separation of the hemispheres, each hemisphere was bisected coronally at the start of the caudal pole of the diencephalon and frozen in liquid nitrogen at-40°C. Tissue was cryosectioned in the parasagittal plane at-20°C to obtain 40µm for free-floating sections stored in cyroprotectant solution (50% PBS 0.1M, 20% ethylene glycol, 30% glycerol).…”

Section: Methodsmentioning

confidence: 99%

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Dynamic fibroblast-immune interactions shape wound healing after brain injury

Ewing-Crystal,

Mroz,

Chang

et al. 2024

Preprint

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Fibroblasts coordinate the response to tissue injury, directing organ regeneration versus scarring. In the central nervous system (CNS), fibroblasts are uncommon cells enriched at tissue borders, and their molecular, cellular, and functional interactions after brain injury are poorly understood. Here we define the fibroblast response to sterile brain damage across time and space. Early pro-fibrotic myofibroblasts infiltrated CNS lesions and were functionally and spatially organized by fibroblast TGFβsignaling, pro-fibrotic macrophages and microglia, and perilesional brain glia that activated TGFβvia integrinαvβ8. Early myofibroblasts subsequently transitioned into a variety of late states, including meningeal and lymphocyte-interactive fibroblasts that persisted long term. Interruption of this dynamic fibroblast-macrophage-glial coordination impaired brain wound healing and the resolution of neuroinflammation, disrupted generation of latede novoCNS lymphocyte niches, and increased mortality in a stroke model. This work highlights an unexpected role of fibroblasts as coordinate regulators of CNS healing and neuroinflammation after brain injury.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Dynamic fibroblast-immune interactions shape wound healing after brain injury

Ewing-Crystal,

Mroz,

Chang

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

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Axon Guidance Molecules and Pain

Damo

Simonetti

2022

Cells

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Chronic pain is a debilitating condition that influences the social, economic, and psychological aspects of patients’ lives. Hence, the need for better treatment is drawing extensive interest from the research community. Developmental molecules such as Wnt, ephrins, and semaphorins are acknowledged as central players in the proper growth of a biological system. Their receptors and ligands are expressed in a wide variety in both neurons and glial cells, which are implicated in pain development, maintenance, and resolution. Thereby, it is not surprising that the impairment of those pathways affects the activities and functions of the entire cell. Evidence indicates aberrant activation of their pathways in the nervous system in rodent models of chronic pain. In those conditions, Wnt, ephrin, and semaphorin signaling participate in enhancing neuronal excitability, peripheral sensitization, synaptic plasticity, and the production and release of inflammatory cytokines. This review summarizes the current knowledge on three main developmental pathways and their mechanisms linked with the pathogenesis and progression of pain, considering their impacts on neuronal and glial cells in experimental animal models. Elucidations of the downstream pathways may provide a new mechanism for the involvement of Wnt, ephrin, and semaphorin pathways in pain chronicity.

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Replicating infant-specific reactive astrocyte functions in the injured adult brain

Cited by 2 publications

References 87 publications

Dynamic fibroblast-immune interactions shape wound healing after brain injury

Dynamic fibroblast-immune interactions shape wound healing after brain injury

Axon Guidance Molecules and Pain

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