Reparation as a Factor Contributing to Genetic Variability of the Biological System

Abstract

Kinetics of DNA synthesis in mitotic cycle of in mouse corneal epithelial cells after γ-irradiation in different S phase points was studied by the method of autoradiography. Normally, S phase of corneal epithelial cells consists of two phases (S1 and S2) separated by an interval without DNA synthesis. Each phase, in turn, includes two subphases with a pause in DNA synthesis. It was hypothesized that pauses in DNA synthesis, similar to that during presynthetic g1 phase, correspond to periods of cell preparation to the next stage of their development. After irradiation, reparation proceeds during pauses at different phases of the cell cycle. The mechanism of reparation induces cell genome rearrangement.