Remote triggering of TGF-β/Smad2/3 signaling in human adipose stem cells laden on magnetic scaffolds synergistically promotes tenogenic commitment

Matos, Ana M.; Gonçalves, Ana I.; Rodrigues, Márcia T.; Miranda, Miguel; Haj, Alicia J. El; Reis, Rui L.; Gomes, Manuela E.

doi:10.1016/j.actbio.2020.07.009

Cited by 15 publications

(10 citation statements)

References 42 publications

(39 reference statements)

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“…4A). This finding is in line with a work showing a mechano-sensitive role of ACTR-IIA in tenogenic commitment of adipose stem cells under magnetic stimulation 53 .…”

Section: Stiffness Dependence Of Bmps and Bmp Receptors In Cell Adhesion And Spreadingsupporting

confidence: 92%

Differential bioactivity of four BMP-family members as function of biomaterial stiffness

Sales¹,

Khodr²,

Machillot³

et al. 2022

Biomaterials

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Whereas soft biomaterial is not able to induce cell spreading, BMP-2 presented by a soft film has been described to be sufficient to trigger cell spreading, migration and downstream BMP-2 signaling. Based on thin polyelectrolyte films of controlled stiffness, we investigated whether the presentation of four BMP members (2, 4, 7, 9) in a matrix-bound manner may differentially impact cell adhesion and bone differentiation of skeletal progenitors. We performed high content and automated screening of cellular responses, including cell number, cell spreading area, SMAD phosphorylation and alkaline phosphatase activity. The basolateral presentation of the different BMPs allowed us to discriminate the specificity of cellular response and the role of BMP receptors type I, type II, as well as three β integrins, in a BMP type and stiffness-dependent manner..

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“…4A). This finding is in line with a work showing a mechano-sensitive role of ACTR-IIA in tenogenic commitment of adipose stem cells under magnetic stimulation 53 .…”

Section: Stiffness Dependence Of Bmps and Bmp Receptors In Cell Adhesion And Spreadingsupporting

confidence: 92%

Differential bioactivity of four BMP-family members as function of biomaterial stiffness

Sales¹,

Khodr²,

Machillot³

et al. 2022

Biomaterials

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“…This scaffold was based on a biodegradable polymeric blend of starch and MNP‐incorporated PCL. [ 142 ] Under AMF, the magnetic actuators synergistically triggered the ActRIIA, and a subsequent induction of the transforming growth factor‐β (TGF‐β) signaling pathway followed, through the Smad2/3 phosphorylation cascade. Consequently, the expression of tendon‐related genes was augmented, along with the deposition, phosphorylation, and nuclear colocalization of the resulting proteins.…”

Section: Cell Processes Affected By Magnetismmentioning

confidence: 99%

“…[ 116,140,141 ] For example, tendon and ligament‐mimetic scaffolds have been functionalized with MNPs to target cellular mechanosensor. [ 141,142 ] In one study, the magnetoactuation induced the anisotropic organization of the cytoskeleton in human ASCs (hASCs). [ 141 ] A mechanically reinforced and magnetically responsive substrate for cell growth was derived from a PCL matrix filled with rod‐shaped cellulose nanocrystals decorated with IONPs.…”

Section: Cell Processes Affected By Magnetismmentioning

confidence: 99%

Engineered Magnetic Nanocomposites to Modulate Cellular Function

Filippi

Garello

Yaşa

et al. 2021

Small

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Magnetic nanoparticles (MNPs) have various applications in biomedicine, including imaging, drug delivery and release, genetic modification, cell guidance, and patterning. By combining MNPs with polymers, magnetic nanocomposites (MNCs) with diverse morphologies (core–shell particles, matrix‐dispersed particles, microspheres, etc.) can be generated. These MNCs retain the ability of MNPs to be controlled remotely using external magnetic fields. While the effects of these biomaterials on the cell biology are still poorly understood, such information can help the biophysical modulation of various cellular functions, including proliferation, adhesion, and differentiation. After recalling the basic properties of MNPs and polymers, and describing their coassembly into nanocomposites, this review focuses on how polymeric MNCs can be used in several ways to affect cell behavior. A special emphasis is given to 3D cell culture models and transplantable grafts, which are used for regenerative medicine, underlining the impact of MNCs in regulating stem cell differentiation and engineering living tissues. Recent advances in the use of MNCs for tissue regeneration are critically discussed, particularly with regard to their prospective involvement in human therapy and in the construction of advanced functional materials such as magnetically operated biomedical robots.

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“…106 This work showed promise outcomes in the commitment of human adipose stem cells towards tenogenesis and has been recently taken forward heading to a mechanostimulation platform in mediating mechanotransduction effects through TGF-b/Smad2/3 signalling. 111 Other fiber production methods as electrospinning have been pursued in advancing magnetically responsive scaffolds. Magnetic yarns of continuous and aligned electrospun threads of PCL and cellulose nanocrystals (CNCs) have been recently reported by us to activate YAP/TAZ signalling in response to magnetic stimulus.…”

Section: Advancing Spions Based Strategies Into Tissue Engineering and Regenerative Medicinementioning

confidence: 99%

Magnetic triggers in biomedical applications – prospects for contact free cell sensing and guidance

et al. 2021

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Remote triggering of TGF-β/Smad2/3 signaling in human adipose stem cells laden on magnetic scaffolds synergistically promotes tenogenic commitment

Cited by 15 publications

References 42 publications

Differential bioactivity of four BMP-family members as function of biomaterial stiffness

Differential bioactivity of four BMP-family members as function of biomaterial stiffness

Engineered Magnetic Nanocomposites to Modulate Cellular Function

Magnetic triggers in biomedical applications – prospects for contact free cell sensing and guidance

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