Remarks on the Vitality of the Human Cornea After Organ Culture

Salla, Sabine; Redbrake, Claudia; Becker, Jakob; Reim, Martin

doi:10.1097/00003226-199509000-00010

Cited by 32 publications

(20 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Most authors agree that corneas require a minimum period of storage in culture medium, called quarantine, in order to maximise the elimination of contaminated corneas: Erbezci et al 10 recommend at least 8 days and Laroche27 at least 14 days. Conversely, it is established that endothelial cell density, which is one of the main factors in the long term survival of the graft, drops as storage time rises 28. Consequently, cutting storage time without compromising microbiological safety seems an important objective for corneal bankers.…”

Section: Discussionmentioning

confidence: 99%

Use of a pair of blood culture bottles for sterility testing of corneal organ culture media

Gain

Thuret²,

Chiquet³

et al. 2001

British Journal of Ophthalmology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Use of a pair of blood culture bottles for sterility testing of corneal organ culture media

Gain

Thuret²,

Chiquet³

et al. 2001

British Journal of Ophthalmology

View full text Add to dashboard Cite

“…According to the two-phase OC method established by Pels et al [3] and used thereafter throughout Europe, corneas are firstly stored in a dextran-free medium for the main storage period, then transferred to a medium containing 5–8% dextran T500 for deswelling between 1 and 7 days before graft. However, the inocuity of dextran-containing media to corneal endothelial cells (ECs) initially described [4,5,6] has been questioned several times. An average of 8.4% EC loss was reported after 1–4 days of deswelling in an OC medium supplemented with 5% dextran T500 [7].…”

Section: Introductionmentioning

confidence: 99%

Poloxamines for Deswelling of Organ-Cultured Corneas

et al. 2012

View full text Add to dashboard Cite

Background: Poloxamines are amphiphilic tetrofunctional block copolymers composed of four polyoxyethylene-polyoxypropylene arms joined to a central ethylene diamine bridge. Their safe profile allows diverse pharmaceutical and biomedical applications. Aim: To assess their use for corneal deswelling using a porcine model of organ culture (OC). Methods: Five poloxamines (T90R4, T904, T908, T1107 and T1307) were dissolved in a standard commercial OC medium (control) to reach 350 mosm kg^–1. In vitro cytotoxicity was tested using MTT assay on human corneal epithelial and endothelial cell (EC) lines and on primary human corneal fibroblasts. Paired porcine corneas stored in OC for 3 days were assigned for 48 h to a poloxamine medium or to a standard deswelling medium containing 5% dextran T500. Corneal EC density, morphometry, mortality, stromal thickness and transparency were evaluated before and after deswelling. Post-deswelling, EC viability/mortality was determined using a fluorescent live/dead assay. Results: Besides similar corneal thickness reduction and transparency improvement, T908, T1107 and T1307 decreased EC loss (5.4 ± 1.7% vs. 9.9 ± 2.6% in controls (p < 0.001)) and mortality, improved EC morphometry and reduced endothelial lesions compared to dextran. Conclusion: On this porcine model, poloxamines T908, T1107 and T1307 appear as good candidates to replace dextran for the deswelling. Experiments on human corneas are now necessary to confirm their efficiency and safety profile in OC.

show abstract

“…On one hand, some latent infections may be inhibited by the fresh antibiotic dose in the deswelling medium, but others may be boosted by the fresh nutrient supply or activated by the deswelling process itself [35]. On the other hand, it is known that Dextran 500 contained in the deswelling medium displays toxicity [5,17,23,25,36], and that the deswelling duration should be kept to the necessary minimum. If a sterility test is carried out at the time of placement into the deswelling medium or thereafter, the rapid contamination warning is essential.…”

Section: Discussionmentioning

confidence: 99%