Reliability of fourier transform infrared spectroscopy in the characterization of human skin

Longas, Marı́a O.; Cheairs, Kenya; Puchalski, Michelle M.; Park, Jung I.

doi:10.4236/abc.2011.12004

Cited by 6 publications

(2 citation statements)

References 22 publications

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“…Crude GAG present in O. stamineus showed bands between 1540 cm −1 and 1500 cm −1 , suggesting protein residual [13]. The spectrum in the 3413-3250 cm −1 region was originated from hydroxyl group (O-H), such as those that can be found in glucuronic acid [17]. Stretching vibrations of S=O for sulfates ( − SO 3 ) were detected at several spectra in the range of 1312-1260 cm −1 from the crude sample, similar to the standard that is identical to GAG's characteristics [18].…”

Section: Resultsmentioning

confidence: 99%

“…Characterization by FTIR analysis confirmed the identity of O. stamineus crude extract as an aliphatic compound due to the presence of spectrums below 3000 cm −1 wavenumbers. FTIR can be used to identify the organic functional groups contained in the crude extract, allowing for rapid and reliable detection in diverse sample types [17,47]. The O. stamineus crude extract showed several absorbance peaks that indicate amine, carboxyl, hydroxyl, carbonyl, and sulfate groups.…”

Section: Discussionmentioning

confidence: 99%

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A Rapid Quantitative Dye-Binding Method of Screening Glycosaminoglycans Presence in Medicinal Plants

Zahari

Sham

Shahabudin

et al. 2019

Asian J Pharm Clin Res

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Objectives: The aims of this paper are to extract glycosaminoglycan (GAG) from four local medicinal plants and to characterize the crude extract with highest total sulfated GAG to reduce the dependency of using animals as major sources.Methods: Crude GAG was extracted from four plants (Gaultheria procumbens, Strobilanthes crispus, Orthosiphon stamineus, and Ficus deltoidea) using hot water extraction with some modifications. Ultraviolet (UV) spectrophotometry was conducted for purity test. Total sulfated GAG was determined using Blyscan assay kit. By comparing results between the extract yields and total sulfated GAG, the plant consisting of high total sulfated GAG was chosen for further characterization. The selected plant sample was examined by microscopy and further analyzed by nuclear magnetic resonance (NMR) and Fourier-transform infrared (FTIR) spectroscopy.Results: All four plants showed absorbance peaks between 214 and 232 nm in UV scan that represented negatively charged sugar. O. stamineus was found to contain the highest amount of sulfated GAG, 62.63±0.01 μg/mg by Blyscan assay. Microscopical examination confirmed the identity of O. stamineus sample by comparing to the reference. Both NMR and FTIR analysis of O. stamineus crude yield showed the presence of hydroxyl, sulfates, carboxylate, and amine groups, suggesting close resemblances to GAG structure.Conclusion: The results suggested that all four plants contained GAG compound. O. stamineus was found to exhibit the most abundant total sulfated GAG and has the potential to become a new plant-based source for GAG.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%