Relevance of tracking the diversity of Escherichia coli pathotypes to reinforce food safety

Enciso‐Martínez, Yessica; González‐Aguilar, Gustavo A.; Martínez-Téllez, Miguel Ángel; González‐Pérez, Cristóbal J.; Valencia, Dora; Barrios-Villa, Edwin; Ayala‐Zavala, J. Fernando

doi:10.1016/j.ijfoodmicro.2022.109736

Cited by 19 publications

(14 citation statements)

References 92 publications

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“…During food manufacturing, contamination can occur from various sources, the main ones being the hands of collaborators, the environment, poorly sanitized utensils, and equipment (Enciso-Martínez et al, 2022). Meat products are also susceptible to contamination from animal hides if sanitary procedures are not performed correctly during slaughter (Sagawa et al, 2022).…”

Section: Tablementioning

confidence: 99%

Acompanhamento da microbiota indicadora e patogênica durante a vida útil de Longissimus dorsi (contrafilé) embalado a vácuo

Mendonça,

Nunes,

Meurer

et al. 2024

Sem. Ci. Agr.

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O Brasil é um dos maiores produtores de carne do mundo. Tendo em vista essa grande produtividade e a preocupação com a qualidade da carne produzida, os estabelecimentos produtores estão buscando meios para maior conservação do produto, sendo a embalagem a vácuo um dos mais utilizados. O objetivo do trabalho foi acompanhar a microbiota indicadora e patogênica durante a shelf life de Longissimus dorsi bovino embalado a vácuo. Foram avaliadas amostras de contrafilé coletadas e acondicionadas na seção de desossa de um frigorífico sob inspeção federal. Cada amostra foi fracionada em quatro peças e cada peça foi utilizada para compor uma parte de cada um dos pools, sendo totalizado quatro pools mantidos à 7ºC e analisados de 0 até os 60 dias de embalagem primária, com intervalo de 20 dias. Foram quantificados aeróbios mesófilos, psicrotróficos, enterobactérias, coliformes a 30°C, Escherichia coli e Staphylococcus spp. Através de abordagens moleculares foram caracterizados os patótipos de E. coli produtora da toxina shiga (STEC), enteropatogênica (EPEC), enterohemorrágica (EHEC), enteroagregativa (EAEC), enterotoxigênica (ETEC) e enteroinvasiva (EIEC), Pseudomonas spp. entre os psicrotróficos, Salmonella spp. e Listeria monocytogenes. As quantificações dos micro-organismos indicadores foram aumentando progressivamente a cada intervalo de análise, com destaque para os psicrotróficos que aumentaram de 5 x 101 no dia 0 para 4,2 x 108 UFC/g no dia 60, predominando Pseudomonas spp. (48%). As contagens que possuem padrão determinado por legislações vigentes tiveram seus limites ultrapassados, como E. coli, desde o dia 20 (7 x 102 UFC/g). Foram identificadas EPEC, ETEC, STEC e EIEC, além de L. monocytogenes em todas as análises e Salmonella spp., essa última só não detectada no primeiro dia de shelf life. Fazem-se necessárias, portanto, revisões nos planos de autocontrole assim como maior rigor microbiológico na produção e processamento da carne bovina para melhoria da shelf life do produto e aumento da sua segurança.

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Section: Tablementioning

confidence: 99%

Acompanhamento da microbiota indicadora e patogênica durante a vida útil de Longissimus dorsi (contrafilé) embalado a vácuo

Mendonça,

Nunes,

Meurer

et al. 2024

Sem. Ci. Agr.

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“…Therefore, in most countries, research must be expanded on monitoring and characterizing bacterial antibiotic resistance. This action is relevant because it would allow for a better understanding of this global challenge [ 27 ]. This situation represents a challenge since a correct antibiotic-resistance characterization is needed, but it is a long process.…”

Section: Antibiotic Bacterial Resistance: a Serious And Multidiscipli...mentioning

confidence: 99%

Facing Resistant Bacteria with Plant Essential Oils: Reviewing the Oregano Case

et al. 2022

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Antibiotic resistance is a serious global threat, and the misuse of antibiotics is considered its main cause. It is characterized by the expression of bacterial defense mechanisms, e.g., β-lactamases, expulsion pumps, and biofilm development. Acinetobacter baumannii and Pseudomonas aeruginosa are antibiotic-resistant species that cause high morbidity and mortality. Several alternatives are proposed to defeat antibiotic resistance, including antimicrobial peptides, bacteriophages, and plant compounds. Terpenes from different plant essential oils have proven antimicrobial action against pathogenic bacteria, and evidence is being generated about their effect against antibiotic-resistant species. That is the case for oregano essential oil (Lippia graveolens), whose antibacterial effect is widely attributed to carvacrol, its main component; however, minor constituents could have an important contribution. The analyzed evidence reveals that most antibacterial evaluations have been performed on single species; however, it is necessary to analyze their activity against multispecies systems. Hence, another alternative is using plant compounds to inactivate hydrolytic enzymes and biofilms to potentiate antibiotics’ effects. Despite the promising results of plant terpenes, more extensive and deep mechanistic studies are needed involving antibiotic-resistant multispecies to understand their full potential against this problem.

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“…5, 6 Processed meat and fresh vegetables are listed as risk factors for EHEC infection. 7, 8 Symptoms of EHEC infection include mild watery diarrhea, hemorrhagic colitis, and, in severe cases, hemorrhagic uremic syndrome, which is associated with a high mortality rate. 1, 2 However, antibiotic treatment is not recommended to cure EHEC infections, because it has been identified as a high-risk factor for hemorrhagic uremic syndrome.…”

Section: Introductionmentioning

confidence: 99%

Quinoclamine inhibits Shiga toxin production in enterohemorrhagicEscherichia coli

Kar,

Feng,

Hirano

et al. 2023

Preprint

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ObjectivesEnterohemorrhagicEscherichia coli(EHEC) is responsible for the most severe symptoms ofE. coliinfections, including hemorrhagic colitis and hemorrhagic uremic syndrome. Shiga toxin 2 (Stx2) plays a significant role as a major virulence factor. The genes encoding Stx2 locate in lambda-like prophage on the EHEC genome. Consequently, Stx2 is expressed when production of the phage is induced by the SOS response. Antibiotic treatment is not recommended for curing the bacterial infection, because it is associated with severe hemorrhagic uremic syndrome. If Stx2 production is prevented, EHEC pathogenicity significantly decreases, and antibiotics may be available to treat the infection.MethodsWe conducted two independent screenings to identify Stx2 production inhibitors for libraries from the RIKEN Natural Product Depository (NPDepo); namely, screening of the Authentic Library, and two-round screening of the Pilot and Analog Libraries.ResultsThe screening of Authentic Library identified niclosamide as a Stx2 production inhibitor. Besides, two naphthoquinoids were identified after the two-round of screening of the Pilot and Analog Libraries. Niclosamide, and quinoclamine, which has structure shared in the two naphthoquinoids, prevented cell lysis via the phage production and ceased Stx2 production in EHEC. The SOS reporter assay indicated that quinoclamine prevented the SOS response inE. coli, whereas niclosamide did not.ConclusionsThese findings suggest that quinoclamine inhibited Stx2 production by preventing the SOS response, whereas niclosamide was involved in phage propagation following the SOS response. These compounds can be a potential therapeutic option to treat EHEC infections.

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Relevance of tracking the diversity of Escherichia coli pathotypes to reinforce food safety

Cited by 19 publications

References 92 publications

Acompanhamento da microbiota indicadora e patogênica durante a vida útil de Longissimus dorsi (contrafilé) embalado a vácuo

Acompanhamento da microbiota indicadora e patogênica durante a vida útil de Longissimus dorsi (contrafilé) embalado a vácuo

Facing Resistant Bacteria with Plant Essential Oils: Reviewing the Oregano Case

Quinoclamine inhibits Shiga toxin production in enterohemorrhagicEscherichia coli

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