Release of protoplasts from a fungus as a criterion of mechanical interactions in the fermentor

Musílková, M.; Ujcová, E.; Placek, J.; Fencl, Z.; Seichert, L.

doi:10.1002/bit.260230219

Cited by 4 publications

(1 citation statement)

References 5 publications

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“…Environmental conditions as well as changes in the cell wall structure may prevent this exposure of the cell wall glucans to zymolyase and, hereby, inhibit protoplastization. It was, previously, reported that yeasts may respond to various environmental conditions and for this reason the nature of the growth medium and culture condition are of pure importance concerning protoplast production (13,17).…”

Section: Discussionmentioning

confidence: 99%

Electronmicroscopic Observation on Protoplastization of Saccharomyces cerevisiae (uvarum) and Candida albicans under Calcium Deficiency. Elektronenmikroskopische Untersuchungen zur Protoplastenbildung an Saccharomyces cerevisiae (uvarum) und Candida albicans unter Kalziummangel

Berdicevsky

Melchinger²,

Jaeger

et al. 1987

Mycoses

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Summary: Cells of Saccharomyces cerevisiae 21 (S. uvarum) and Candida albicans which were grown in a chemically defined medium with and without additional calcium ions for 12 h and treated with zymolyase turned into protoplasts. But when grown under calcium deficiency for 24 h only C. albicans formed protoplasts, whereas S. cerevisiae 21 failed to do so. In this specific strain other physiological and ultrastructural changes are observed too. The results may lead to the conclusion that calcium deficiency specifically induces marked changes of the cell wall surface in certain yeast strains thereby masking the lytic sites or making them unaccessible for the enzyme. Zusammenfassung: Zellen von Saccharomyces cerevisiae 21 (S. uvarum) und Candida albicans, die 12 h lang in einem chemisch definierten Medium mit und ohne Kalzium‐Ionen gewachsen und mit Zymolyase behandelt worden waren, wandelten sich in Protoplasten um. Wurden die Zellen dagegen 24 h unter Kalzium‐Mangel gehalten, wandelten sich nur die Candida albicans‐Zellen, nicht jedoch S. cerevisiae, in Protoplasten um. In dem letztgenannten Stamm wurden auch andere physiologische und ultra‐strukturelle Eigenschaften beobachtet. Die Ergebnisse lassen den Schluß zu, daß Kalzium‐Mangel in bestimmten Hefestämmen zu deutlichen Veränderungen der Zellwandoberfläche führt, wodurch die Lyse‐Angriffspositionen maskiert oder für das Enzym unzugänglich gemacht werden.

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Section: Discussionmentioning

confidence: 99%

Electronmicroscopic Observation on Protoplastization of Saccharomyces cerevisiae (uvarum) and Candida albicans under Calcium Deficiency. Elektronenmikroskopische Untersuchungen zur Protoplastenbildung an Saccharomyces cerevisiae (uvarum) und Candida albicans unter Kalziummangel

Berdicevsky

Melchinger²,

Jaeger

et al. 1987

Mycoses

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Der Laborkleinfermentor LF2 – Physikalische Kenndaten, Beurteilung der Originalkonfiguration und Charakterisierung spezieller Ausrüstungsvarianten

Große¹,

Reichenbach

Bocker³

1985

Acta Biotechnologica

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The stirred laboratory scale fermenter LF2 is a powerful device for fermentations with up to 3 liters culture broth [1]. It was designed in the Academy of Sciences of the C.S.S.R. and has found a rather wide application in a great number of organizations working in the fields of biotechnological education, research and industry in the G.D.R. and the C.S.S.R.The LF2‐fermenter is well suitable for carrying out a broad manifold of aerated biotechnological works without contaminations in basic research and process development.The mechanic and electronic design gives a lot of room for the realization of special equipment variants like special‐shaped reactor vessels or special electronic moduls for automatic control.

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Fungal morphology and metabolite production in submerged mycelial processes

Papagianni¹

2004

Biotechnology Advances

718

505

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Release of protoplasts from a fungus as a criterion of mechanical interactions in the fermentor

Cited by 4 publications

References 5 publications

Electronmicroscopic Observation on Protoplastization of Saccharomyces cerevisiae (uvarum) and Candida albicans under Calcium Deficiency. Elektronenmikroskopische Untersuchungen zur Protoplastenbildung an Saccharomyces cerevisiae (uvarum) und Candida albicans unter Kalziummangel

Electronmicroscopic Observation on Protoplastization of Saccharomyces cerevisiae (uvarum) and Candida albicans under Calcium Deficiency. Elektronenmikroskopische Untersuchungen zur Protoplastenbildung an Saccharomyces cerevisiae (uvarum) und Candida albicans unter Kalziummangel

Der Laborkleinfermentor LF2 – Physikalische Kenndaten, Beurteilung der Originalkonfiguration und Charakterisierung spezieller Ausrüstungsvarianten

Fungal morphology and metabolite production in submerged mycelial processes

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