Release of kinin by an enzyme in the accessory sex glands of the guinea‐pig

The subcellular localization of kallikrein, amylase and acetylcholine in the submaxillary gland of the guinea‐pig

“…This view has been strongly supported by the experimental work of Hilton & Lewis (1955a, b, 1956 Lewis, 1959;Hilton, 1960), who concluded that both cholinergic and adrenergic vasodilation in this organ was produced by kallikrein. Recently much evidence has accumulated which does not support this hypothesis (Bhoola, May May Yi, Morley & Schachter, 1962;Beilson, Schachter & Smaje, 1965; Bhoola, Morley, Schachter & Smaje, 1965). The physiologic function of kallikrein remains unknown.…”

The major androgen‐dependent protease in dog prostate belongs to the kallikrein family: confirmation by partial amino acid sequencing

“…220 amino acids. 4. DISCUSSION the disulfide bridges, two chains corresponding to 15 kDa and 14 kDa, respectively, were released, Following the study concerning the effect of the former being able to bind DFP while the latter, testicular hormones on synthesis of soluble proin view of its electrophoretic heterogeneity, is proteins by dog prostate slices, it was proposed that bably glycosylated [6].…”

“…Since it is not yet known whether the canine enzyme is able to release kinins (preliminary data [4,5] seem to indicate that it cannot), its similarity to the y-subunit together with the high amount present in the prostate gland are intriguing. The prostate gland of guinea pig, rabbit and bull were shown to contain substantial amount of NGF [22] but it is not known whether NGF is present in substantial amount in the prostate gland of the dog.…”

“…Indeed, some early reports [2,3] have described the presence in canine prostatic fluids of numerous proteolytic activities including some akin to an Arg-esterase activity. Similarly, a kallikrein-like enzyme or kininogenase, as evidenced by its ability to generate kallidin from crude dog kininogen and to induce a significant vasodilator effect in dog, was shown to be present in the accessory sex glands and in the prostate gland of the guinea pig [4]. However, such a kinin-producing activity was not observed in the case of the enzymes present in the coagulating gland of the rat or in the prostate gland of rat, rabbit, dog and man [4].…”

“…Similarly, a kallikrein-like enzyme or kininogenase, as evidenced by its ability to generate kallidin from crude dog kininogen and to induce a significant vasodilator effect in dog, was shown to be present in the accessory sex glands and in the prostate gland of the guinea pig [4]. However, such a kinin-producing activity was not observed in the case of the enzymes present in the coagulating gland of the rat or in the prostate gland of rat, rabbit, dog and man [4]. Recently, one of the major protein constituents of canine seminal fluid was found to be rapidly synthesized when canine prostatic slices were incubated with radioactive amino acids; furthermore, both the amount synthesized and the kinetics were greatly affected by the level of testicular hormones as evidenced in castrated animals and upon injection of androgens [5].…”