Relationships between Schistosoma malayensis and other Asian schistosomes deduced from DNA sequences

Blair, David; Herwerden, Lynne van; Hirai, Hirohisa; Taguchi, Takahiro; Habe, Shigehisa; Hirata, Mamoru; Lai, Karen; Upatham, Suchart; Agatsuma, Takeshi

doi:10.1016/s0166-6851(96)02827-7

Cited by 40 publications

(20 citation statements)

References 19 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(3S) and a reverse primer 5'-CCTGGTTAGTTTCTTTTCCTCCGC-3' (A28) (Bowles et al, 1995;Blair et al, 1997). A portion of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) was also amplified using a forward primer 5'-TGGTTTTTTGTGCATCCTGAGGTTTA-3' (pr-a) and a reverse primer 5'-AGAAAGAACGTAATGAAAATGAGCAAC-3' (pr-b) (Okamoto et al, 1995).…”

Section: Molecular Identification and Phylogenetic Analysis Of The Lumentioning

confidence: 99%

Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations

Nkouawa

Okamoto

Mabou³

et al. 2009

Transactions of the Royal Society of Tropical Medicine and Hygi

Self Cite

View full text Add to dashboard Cite

SummaryParagonimiasis is a common parasitic zoonosis in Cameroon and neighboring countries in Western Africa. Serum, sputum and faecal samples were collected in an endemic area of South West Province, Cameroon, after administration of a questionnaire to identify individuals with appropriate symptoms and histories. Microscopic examination revealed eggs in sputum from 16 people, but none in any faecal sample. These 16 were among the 25 and 26 people respectively positive by enzyme-linked immunosorbent assay (ELISA) and by immunoblot using Paragonimus africanus crude antigens. The detection of copro-DNA by polymerase chain reaction (PCR) was attempted using 23 faecal samples (18 from sputum egg-negative and 5 from sputum egg-positive individuals). The copro-DNA was detected in 4 of the 5 sputum eggpositive individuals. These results suggest that (1) serology is much more sensitive than sputum examination for diagnosis of paragonimiasis, and (2) a copro-DNA test may be more sensitive than a microscopic search for eggs in faeces. Molecular sequence data from ITS2 and cox1 genes confirmed that adult worms experimentally raised in cats were P. africanus and that eggs from sputum or other worm products from human faeces also belonged to this species. Based on these results, 26 of 168 persons (15.5%) were diagnosed as suffering from paragonimiasis.

show abstract

Section: Molecular Identification and Phylogenetic Analysis Of The Lumentioning

confidence: 99%

Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations

Nkouawa

Okamoto

Mabou³

et al. 2009

Transactions of the Royal Society of Tropical Medicine and Hygi

Self Cite

View full text Add to dashboard Cite

show abstract

“…Most recent studies have added gene fragments or additional taxa to particular clades within Schistosoma to test the position of individual taxa (e.g. Agatsuma et al 2001Agatsuma et al , 2002Blair et al 1997), or to examine some of the biogeographic hypotheses suggested by Snyder & Loker's (2000) scheme, e.g. Zhang et al (2001) and Attwood et al (2002 b).…”

Section: Taxonomy and Systematicsmentioning

confidence: 99%

The phylogeny of the Schistosomatidae based on three genes with emphasis on the interrelationships of Schistosoma Weinland, 1858

et al. 2003

View full text Add to dashboard Cite

S U M M A R YSchistosomes are digenean flukes, parasitic of birds, mammals and crocodiles. The family Schistosomatidae contains species of considerable medical and veterinary importance, which cause the disease schistosomiasis. Previous studies, both morphological and molecular, which have provided a good deal of information on the phylogenetics of this group, have been limited in the number of species investigated or the type or extent of molecular data used. This paper presents the most comprehensive phylogeny to date, based on the sequences of 3 genes, complete ribosomal small subunit rRNA and large ribosomal subunit rRNA, and mitochondrial cytochrome oxidase 1, sequenced from 30 taxa including at least 1 representative from 10 of the 13 known genera of the Schistosomatidae and 17 of the 20 recognized Schistosoma species. The phylogeny is examined using morphological characters, intermediate and definitive host associations and biogeography. Theories as to the origins and spread of Schistosoma are also explored. The principal findings are that Ornithobilharzia and Austrobilharzia form a sister group to the Schistosoma ; mammalian schistosomes appear paraphyletic and 2 Trichobilharzia species, T. ocellata and T. szidati, seem to be synonymous. The position of Orientobilharzia within the Schistosoma is confirmed, as is an Asian origin for the Schistosoma, followed by subsequent dispersal through India and Africa.

show abstract

“…Sequences from two other schistosomes of mammals, Schistosomatium douthitti and Orientobilharzia turkestanicum, were used as outgroups for the ITS2 and 28S data set (Bowles et al, 1995;Snyder & Loker, 2000). Partial CO1 sequences of Paragonimus species (Blair et al, 1997b) were used as outgroups for the CO1 data set.…”

Section: Alignment and Tree Analysismentioning

confidence: 99%

Affinities between Asian non-human Schistosoma species, the S. indicum group, and the African human schistosomes

et al. 2002

View full text Add to dashboard Cite

Schistosoma species have traditionally been arranged in groups based on egg morphology, geographical origins, and the genus or family of snail intermediate host. One of these groups is the`S. indicum group' comprising species from Asia that use pulmonate snails as intermediate hosts. DNA sequences were obtained from the four members of this group (S. indicum, S. spindale, S. nasale and S. incognitum) to provide information concerning their phylogenetic relationships with other Asian and African species and species groups. The sequences came from the second internal transcribed spacer (ITS2) of the ribosomal gene repeat, part of the 28S ribosomal RNA gene (28S), and part of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene. Tree analyses using both distance and parsimony methods showed the S. indicum group not to be monophyletic. Schistosoma indicum, S. spindale and S. nasale were clustered among African schistosomes, while S. incognitum was placed as sister to the African species (using ITS2 and 28S nucleotide sequences and CO1 amino acid sequences), or as sister to all other species of Schistosoma (CO1 nucleotide sequences). Based on the present molecular data, a scenario for the evolution of the S. indicum group is discussed.

show abstract

Relationships between Schistosoma malayensis and other Asian schistosomes deduced from DNA sequences

Cited by 40 publications

References 19 publications

Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations

Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations

The phylogeny of the Schistosomatidae based on three genes with emphasis on the interrelationships of Schistosoma Weinland, 1858

Affinities between Asian non-human Schistosoma species, the S. indicum group, and the African human schistosomes

Contact Info

Product

Resources

About