1982
DOI: 10.1152/ajpgi.1982.242.3.g250
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Abstract: Cholecystokinin (CCK) was conjugated to 125I-Bolton-Hunter reagent (125I-BH-CCK), and the binding of this ligand to CCK receptors in isolated mouse pancreatic acini was correlated with the regulation by CCK of both amylase release and the transport of 2-deoxyglucose and alpha-aminoisobutyric acid. Stimulation of amylase release by CCK was biphasic. At low CCK concentrations (less than 200 pM), amylase release was progressively stimulated, whereas at higher CCK concentrations (greater than 200 pM), amylase rele… Show more

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Cited by 45 publications
(33 citation statements)
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“…The competitive inhibition of['25I]CCK8-bindingto rat pancreatic acini by unlabeled CCK8 was studied. This inhibition curve showed two phases, suggesting that CCK8 interacted with two different affinity sites on rat pancreatic acini as reported previously (25). Taxol (50 gM) did not affect this inhibition curve of['2I]CCK8-binding to rat pancreatic acini (Fig.…”
Section: Resultssupporting
confidence: 82%
“…The competitive inhibition of['25I]CCK8-bindingto rat pancreatic acini by unlabeled CCK8 was studied. This inhibition curve showed two phases, suggesting that CCK8 interacted with two different affinity sites on rat pancreatic acini as reported previously (25). Taxol (50 gM) did not affect this inhibition curve of['2I]CCK8-binding to rat pancreatic acini (Fig.…”
Section: Resultssupporting
confidence: 82%
“…At lower CCK concentrations, amylase release increased progressively with increasing concentrations, whereas, at higher CCK concentrations, amylase release gradually diminished with increasing CCK concentrations. This results in a maximal stimulating CCK concentration of ϳ100 pM (57,122,123). This bell shape may be due to simultaneous occupation in different proportions of high-affinity (K d ϭ 26 pM), low-capacity and low-affinity (K d ϭ 2.2 nM), high-capacity CCK1R states (123).…”
Section: Cck Stimulation Of Exocrine Pancreatic Secretion: Direct Andmentioning
confidence: 98%
“…Moreover, it was recently shown in rodents that the isolated duck pancreatic acini possess both CCK 1 and vasointestinal polypeptide -pituitary adenylate cyclaseactivating polypeptide receptors and that simultaneous activation of both is required for each to play a physiological role (Xiao & Cui, 2004). The stimulatory effects of CCK and analogues on pancreatic enzyme secretion were also observed in guinea-pigs and mice and that are resulted from occupation of the CCK 1 receptor (Sankaran et al 1982;Jensen et al 1989). Indeed, the presence of CCK 1 receptor on rat and mouse pancreatic acinar cells was visualised by immunofluorescence with a specific CCK 1 receptor antibody (Bourassa et al 1999).…”
Section: Global Effects Of Gastrin and Cholecystokinin In The Gutmentioning
confidence: 99%