Relationship of Anti-60 kDa Heat Shock Protein and Anti-Cholesterol Antibodies to Cardiovascular Events

Veres, Amarilla; Füst, George; Śmieja, Marek; McQueen, Matthew; Horváth, Anna; Yi, Qilong; Bı́ró, Adrienn; Pogue, Janice; Romics, L; Karádi, István; Singh, Mahavir; Gnarpe, Judy; Prohászka, Zoltán; Yusuf, Salim

doi:10.1161/01.cir.0000038890.39298.8d

Cited by 64 publications

(39 citation statements)

References 32 publications

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“…No significant correlation was shown between CRP and serum anti-HSP60 IgG levels. This is in agreement with previous reports in the literature which do not demonstrate significant relationship between CRP and serum anti-human HSP60 levels [24,25]. The correlation of CRP and HSP-proliferative responses suggests that inflammation may play a role in human HSP 60-induced lymphoproliferation in CP.…”

Section: Discussionsupporting

confidence: 93%

Immune Responses to HSP65/60 in Periodontal Disease

Hasan¹,

Foo²,

Sadoh³

et al. 2012

JIBTVA

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Chronic periodontitis (CP) is a chronic inflammatory condition which destroys the supporting tissues of teeth and increases in prevalence with age. Immune responses against heat shock proteins (HSP) can be cross-reactive among bacterial and human antigens. There is evidence that microbial HSP65 and human HSP60 are involved in periodontal disease. The aim of this study is to investigate immune responses to the human HSP60 and microbial HSP65 in patients with CP and relate these to the level of inflammation and smoking status. We collected serum samples from 30 patients with chronic gingivitis (CG) and 30 patients with CP. In each group, eight subjects were current smokers. ELISA was used to determine the levels of serum anti-HSP and C-reactive protein (CRP) in each group. Peripheral blood mononuclear cells were also isolated and stimulated with HSPs. Significant lymphoproliferation was seen in CP when stimulated with human HSP60. CRP and serum anti-human HSP60 IgG were elevated in CP compared to the CG, but not serum anti-microbial HSP 65 IgG. In view of the potential confounding effects of smoking in CP, a group of current smokers (n = 16) was also recruited to investigate whether smoking affects HSP immune responses. There was no significant difference in HSP-induced lymphoproliferation between smokers and non-smokers in either the CG or CP. There was a significant correlation between CRP and lymphoproliferative responses to Human HSP60 irrespective of smoking status. This study shows that serum anti-human HSP60 IgG and serum CRP are raised in untreated CP. In CP, serum CRP levels correlated significantly with human HSP60-induced lymphoproliferation, but not with anti-HSP antibody levels.

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Section: Discussionsupporting

confidence: 93%

Immune Responses to HSP65/60 in Periodontal Disease

Hasan¹,

Foo²,

Sadoh³

et al. 2012

JIBTVA

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“…the large inter-individual differences, is probably under the strict control and regulation of genetic factors. Our first observation obtained in a healthy Finnish population identified promoter polymorphism of interleukin-6 as one of the underlying regulatory factors (17), and these results were confirmed in a recent independent study (28). Furthermore, epistatic interaction between immunoglobulin GM allotypes and interleukin-6 was also identified in association with increased anti-Hsp60 antibody levels.…”

Section: Discussionsupporting

confidence: 74%

“…The association of elevated anti-Hsp60 levels with different vascular diseases was repeatedly shown (11), and their clinical importance was also indicated by results of prospective studies (16,17). Most importantly, the causal pathogenic role of antiHsp60 antibodies has been shown by Mandal and associates (18) by showing that anti-Hsp60 antibodies purified from sera of patients with documented atherosclerosis when injected into tail vein of apoE deficient mice resulted in accelerated atherosclerosis in them.…”

mentioning

confidence: 88%

Human anti-60 kD heat shock protein autoantibodies are characterized by basic features of natural autoantibodies

Várbı́ró

Bı́ró

Cervenak

et al. 2010

Acta Physiologica Hungarica

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Anti-human Hsp60 autoantibodies -known risk factor of atherosclerosis -were investigated in a mouse model and in samples of healthy subjects: polyreactivity, presence in cord blood samples of healthy newborns and life-long stability were tested. In IgM hybridoma panel from mouse spleens, polyreactivity of anti-Hsp60 autoantibodies was studied. In healthy pregnant women, umbilical vein and maternal blood samples were collected after childbirth, antiHsp-60 and -65 IgM and IgG levels were measured. Life-long stability of anti-Hsp-60 levels was studied on healthy patients during 5 years. ELISA was used in all studies. Polyreactivity of IgM clones of newborn mice and lifelong stability of these autoantibodies in healthy adults were established. IgM anti-Hsp60 autoantibodies in cord blood of healthy human infants were present, however, there was no correlation between maternal and cord blood IgM anti-Hsp60 concentrations. It is proposed that presence of anti-Hsp60 autoantibodies -as part of the natural autoantibody repertoire -may be an inherited trait. Level of anti-Hsp60 autoantibodies may be an independent, innate risk factor of atherosclerosis for the adulthood.

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“…Hsps are involved in the renaturation of damaged proteins, allowing them to refold into their native conformation. High antibody titres to Hsp-60, -65 and -70 have been reported to be associated with coronary risk factors 7;8 , increased risk of cardiovascular disease 9 , the severity of cardiovascular, and vascular endpoints in patients with established disease 10;11 .…”

Section: Introductionmentioning

confidence: 99%