Relationship between vascular invasion and microvessel density and micrometastasis

Abstract

AIM:To evaluate the relationship between vascular invasion and microvessel density (MVD) of tissue and micrometastasis in blood. M E T H O D S :V a s c u l a r i n v a s i o n w a s d e t e c t e d b y b o t h h e m a t o x y l i n a n d e o s i n s t a i n i n g a n d immunohistochemiscal staining. Blood samples were collected from 17 patients with vascular invasion and 29 patients without vascular invasion and examined for cytokeratin20 (CK20) expression by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Microvessel density of tissue samples was also determined by immunohistochemistry using antibodies to CD105.RESULTS: CK20 was detected in 12 of the 17 patients with vascular invasion and in 9 of the 29 patients without vascular invasion. Positive RT-PCR was significantly correlated with vascular invasion (70.6% vs 30.0%, P < 0.05). The average MVD was significantly higher in patients with positive vascular invasion than in patients with negative vascular invasion (29.2 ± 3.3 vs 25.4 ± 4.7, P < 0.05). The vascular invasion detected with hematoxylin-eosin staining was less than that with immunohistochemical staining. There was a significant difference between the two staining methods (19.6% vs 36.9%, P < 0.05). CONCLUSION:Positive CK20 RT-PCR, depth of tumor invasion, lymph node status, metastasis and MVD are significantly correlated with vascular invasion. Immunohistochemical staining is more sensitive than hematoxylin-eosin staining for detecting vascular invasion.

Study on relationship between angiogenesis and micrometastases of peripheral blood in breast cancer

“…It has been discovered that MVD is closely related to tumor invasive ability, metastasis and prognosis (Yan et al 2008). As a reliable index for quantifying the degree of angiopoiesis, MVD is considered to predict the tumor metastasis in many human tumors (Wang et al 2007a). CD31, a relatively speciWc marker for tumor vascular endothelial, was used to stain microvessels in our study.…”

Anti-proliferative and angio-suppressive effect ofStoechospermum marginatum(C. Agardh) Kutzing extract using various experimental models

“…An earlier study has established that the density of microvessels is almost doubled in tumors from patients with metastasis [46]. While the presence of vascular invasion is not a consistent finding, it is still linked to increased incidence of lymph node, distant metastasis, and a corresponding decrease in survival [47]. …”

Prognostic Value of Microvascular Density in Dukes A and B (T1–T4, N0, M0) Colorectal Carcinomas

“…In the majority of reports, increase in tumour vascularisation favours the growth and remote dissemination of the cancer cells and is, therefore, a predictor of poor outcome in CRC [16–28]. On the other hand, some authors have reported higher survival rates in tumours with high MVD.…”

“…More recently, similar studies have been performed on colorectal cancer (CRC). The results of those studies are controversial because although the majority of them reported an association between a greater microvascular density, remote metastasis, and patient survival [16–28], others did not [29–31], and in some cases, high MVD was associated with better prognosis [32–35]. …”