Relationship between the iron regulated outer membrane proteins and the outer membrane proteins of in vivo grown Pasteurella multocida

Choi-Kim, Keumhwa; Maheswaran, S. K.; Felice, Lawrence J.; Molitor, Thomas W.

doi:10.1016/0378-1135(91)90100-t

Cited by 61 publications

(32 citation statements)

References 37 publications

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“…Several in vivo-expressed surface antigens have been identified as potential vaccine candidates (72,538,571). A number of these are iron-regulated Omps expressed during P. multocida infection and have been characterized as potential immunogens in challenge studies (568)(569)(570)(571)(572)(573). For example, the 96-kDa heme acquisition system receptor (HasR) protein is a surfaceexposed Omp conserved among most P. multocida isolates.…”

Section: Vaccinationmentioning

confidence: 99%

Pasteurella multocida: from Zoonosis to Cellular Microbiology

2013

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SUMMARY In a world where most emerging and reemerging infectious diseases are zoonotic in nature and our contacts with both domestic and wild animals abound, there is growing awareness of the potential for human acquisition of animal diseases. Like other Pasteurellaceae , Pasteurella species are highly prevalent among animal populations, where they are often found as part of the normal microbiota of the oral, nasopharyngeal, and upper respiratory tracts. Many Pasteurella species are opportunistic pathogens that can cause endemic disease and are associated increasingly with epizootic outbreaks. Zoonotic transmission to humans usually occurs through animal bites or contact with nasal secretions, with P. multocida being the most prevalent isolate observed in human infections. Here we review recent comparative genomics and molecular pathogenesis studies that have advanced our understanding of the multiple virulence mechanisms employed by Pasteurella species to establish acute and chronic infections. We also summarize efforts being explored to enhance our ability to rapidly and accurately identify and distinguish among clinical isolates and to control pasteurellosis by improved development of new vaccines and treatment regimens.

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Section: Vaccinationmentioning

confidence: 99%

Pasteurella multocida: from Zoonosis to Cellular Microbiology

2013

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“…Previous investigations of P. multocida have identified iron-regulated outer membrane proteins that are produced in response to iron limitation (8,13,18,25,27,30); however, the genes encoding most of these proteins have not been identified. Importantly, Ruffolo and coworkers have shown that P. multocida grown under low-iron conditions can provide cross-protection against subsequent infections (23).…”

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confidence: 99%

Transcriptional Response of Pasteurella multocida to Defined Iron Sources

et al. 2002

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Pasteurella multocida was grown in iron-free chemically defined medium supplemented with hemoglobin, transferrin, ferritin, and ferric citrate as iron sources. Whole-genome DNA microarrays were used to monitor global gene expression over seven time points after the addition of the defined iron source to the medium. This resulted in a set of data containing over 338,000 gene expression observations. On average, 12% of P. multocida genes were differentially expressed under any single condition. A majority of these genes encoded P. multocida proteins that were involved in either transport and binding or were annotated as hypothetical proteins. Several trends are evident when the data from different iron sources are compared. In general, only two genes (ptsN and sapD) were expressed at elevated levels under all of the conditions tested. The results also show that genes with increased expression in the presence of hemoglobin did not respond to transferrin or ferritin as an iron source. Correspondingly, genes with increased expression in the transferrin and ferritin experiments were expressed at reduced levels when hemoglobin was supplied as the sole iron source. Finally, the data show that genes that were most responsive to the presence of ferric citrate did not follow a trend similar to that of the other iron sources, suggesting that different pathways respond to inorganic or organic sources of iron in P. multocida. Taken together, our results demonstrate that unique subsets of P. multocida genes are expressed in response to different iron sources and that many of these genes have yet to be functionally characterized.

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“…Outer membrane proteins (OMPs) were prepared by using sarcosine (5,36). Briefly, bacteria were harvested in 0.85% (wt/vol) NaCl, washed twice, and resuspended in 10 mM HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid) buffer (pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

“…Porcine isolates of P. multocida usually possess a carbohydrate capsule, which may vary in size (12) and in composition (e.g., capsular types A and D) (3). Other important molecules that are also found at the cell surface of P. multocida are lipopolysaccharides (LPSs), which have endotoxic activities (8), and iron-regulated proteins (IRPs), which are usually expressed in vivo and which are probably implicated in iron transport (5,10,36). Although adhesion seems to be an important step in the pathogenesis of atrophic rhinitis (24), the adhesins of P. multocida are still unknown.…”

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confidence: 99%

Effects of sub-MICs of antibiotics on cell surface characteristics and virulence of Pasteurella multocida

Lebrun

Caya

Jacques

1992

Antimicrob Agents Chemother

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The effects of sub-MICs of certain antibiotics, namely, penicillin G, tetracycline, and trimethoprimsulfamethoxazole, on the cell surface characteristics and the virulences of two toxigenic isolates of Pasteurella multocida representing capsular types A and D were evaluated. Expression of proteins, in particular, outer membrane proteins and iron-regulated proteins, was not affected by exposure of bacterial cells to low concentrations of antibiotics. However, exposition of surface antigens was modified by sub-MICs of the antibiotics tested. The lipopolysaccharide profile of one isolate (capsular type D) was altered by penicillin G. Sub-MICs of penicillin G and tetracycline diminished the virulence ofthe capsular type A isolate and adherence to porcine tracheal rings of the capsular type D isolate. Production of dermonecrotic toxin was not affected by sub-MICs of the antibiotics tested. Our results indicate that growth of P. multocida in the presence of low concentrations of antibiotics seems to have, depending on the isolate, profound effects on cell surface characteristics, with concomitant effects on adherence or virulence. Our results also indicate that production of dermonecrotic toxin, an important virulence factor ofP. multocida isolates associated with porcine atrophic rhinitis, was not affected by sub-MICs of the antibiotics studied.

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Relationship between the iron regulated outer membrane proteins and the outer membrane proteins of in vivo grown Pasteurella multocida

Cited by 61 publications

References 37 publications

Pasteurella multocida: from Zoonosis to Cellular Microbiology

Pasteurella multocida: from Zoonosis to Cellular Microbiology

Transcriptional Response of Pasteurella multocida to Defined Iron Sources

Effects of sub-MICs of antibiotics on cell surface characteristics and virulence of Pasteurella multocida

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