Relationship between the Expression of the Extracellular Matrix Genes SPARC, SPP1, FN1, ITGA5 and ITGAV and Clinicopathological Parameters of Tumor Progression and Colorectal Cancer Dissemination

Abstract

Objective: To evaluate the relationship between the expression of the extracellular matrix (ECM) genes SPARC, SPP1, FN1, ITGA5 and ITGAV and the histopathologic parameters of neoplastic progression and colorectal carcinoma (CRC) dissemination. Methods: A retrospective study was conducted in 114 patients with stage I–IV CRC who underwent primary tumor resection. Quantitative real-time PCR and immunohistochemistry (IHC) assays were performed in samples obtained from the primary tumors. The correlation between the expression of these markers and the expression of p53, Bcl-2, Ki67, epidermal growth factor receptor (EGFR) and vascular endothelial growth factor was assessed with the Spearman coefficient (r). Results: The ITGAV gene was found to be significantly amplified in tumors with positive perineural invasion (p = 0.028). Expression of the SPARC, SPP1, FN1, ITGA5 and ITGAV genes did not correlate with TNM staging. A direct relationship between ITGAV and EGFR expression (r = 0.774; p < 0.001) was observed by IHC. Conclusions: ECM gene expression did not correlate with classical prognostic factors for CRC, but overexpression of the ITGAV gene and protein was correlated with an increased risk of perineural invasion. The relationship between ITGAV and EGFR expression suggests the possibility of crosstalk in this signal pathway.

Integrative Analyses of Hepatic Differentially Expressed Genes and Blood Biomarkers during the Peripartal Period between Dairy Cows Overfed or Restricted-Fed Energy Prepartum

“…At +14 d even fewer predicted up-stream regulators (and down-stream genes) were uncovered by IPA but there was an apparent prominent role and activation in OF vs. RE of osteopontin (SPP1) and metallopeptidase inhibitor 1 (TIMP1) both of which are related to induction of ECM formation [63], [64].…”

Jun-regulated genes promote interaction of diffuse large B-cell lymphoma with the microenvironment

“…Among them, we identified matrix metalloproteinase 7, adhesion molecule CD44, vitronectin receptor (ITGAV), fractalkine receptor (CX3CR1), and transcription factor FoxC1, which are all known to promote the invasion and metastasis of solid tumors. 25,27,[35][36][37][38][39][40] Intriguingly, the increased expression of ITGAV, FoxC1, and CX3CR1 not only correlated with the elevated c-Jun level but also were enriched in secondary extranodal lymphoma. For personal use only.…”

Proteomic Signatures of Acquired Letrozole Resistance in Breast Cancer: Suppressed Estrogen Signaling and Increased Cell Motility and Invasiveness

“…To confirm some of these expression changes we selected several proteins for validation by immunoblotting. ITGAV has been found significantly up-regulated in tumors with positive perineural invasion (p ϭ 0.028) (47) and in breast cancer (48). Viana et al observed a direct relationship between ITGAV and EGFR expression (r ϭ 0.774; p Ͻ 0.001) by IHC, suggesting the possibility of crosstalk in this signaling pathway.…”