Relationship between Oral Anaerobic Bacteria and Otitis Media with Effusion

Abstract

Objective: In this study hypothesing the translocation of oral bacteria from oropharynx into the middle ear cavity may be involved in the pathogenesis of otitis media with effusion (OME), we aimed to investigate the presence and similarity of Fusobacterium nucleatum and Treponema denticola in saliva, nasopharyngeal secretion and the middle ear effusion samples from the children with OME.Methods: Totally 20 children with OME undergoing myringotomy and ventilation tube placement were attended. Stimulated saliva samples were collected after otorhinolaryngological and oral examinations were done. The middle ear effusion and nasopharyngeal secretions were collected during the operations. The presence of F. nucleatum and T. denticola were detected using 16SrRNA-based PCR. The clonal similarities of the bacteria were detected in the samples which the same bacteria had been detected in each samples of the same child. After DNA sequencing, clonal similarity was determined by 16SrRNA gene clone library analysis. The sequences from each clone were compared with similar sequences of reference organisms by FASTA search.Results: T. denticola was detected only in four (20%) saliva and in one (5%) nasopharyngeal sample. F. nucleatum was detected in 11 (55%) saliva, eight (40%) nasopharyngeal and six (30%) middle ear effusion samples. Sequences from F.nucleatum clones derived from three different anatomic sites within patients were similar in 33% of OME patients, indicating their genetic relatedness.Conclusions: Bacteria involved in this process most likely originate from the oropharynx since they show a close genetic relatedness with their oropharyngeal counterparts.

Oral Microbiota in Infants Fed a Formula Supplemented with Bovine Milk Fat Globule Membranes - A Randomized Controlled Trial

“…We concluded that MFGM supplementation provided a protective effect against infections, especially AOM. Bacterial colonization of the upper airways has been suggested to be important for the pathogenesis in AOM [18], and not only nasopharynx cultures [19–21] but also cultures from saliva [22, 23] at onset of AOM correlate to otopathogens…”

Real-Time Polymerase Chain Reaction Versus Conventional PCR: A Comparison between two Methods for the Detection ofFusobacterium Nucleatumin Saliva, Nasopharyngeal Secretion and Middle Ear Effusion Samples

“…thereby, it is well acknowledged that microorganisms from the nasopharynx can reach the middle ear cavity via the eustachian tube and cause middle ear infection. in a previous study, using 16Sr RnA-based conventional PcR, F. nucleatum was detected in 11 (55 %) saliva, eight (40 %) nasopharyngeal and six (30 %) middle ear effusion samples (19). We hypothesize that the rate could be higher if more sensitive methods are used, such as the real-time PcR assay.…”

“…they belonged to a population of a previous study on the relationship between oral anaerobic bacteria and otitis media with effusion (19). Paraffin-stimulated saliva samples were collected immediately after clinical examination.…”

Treponema denticola: patógeno en procesos periodontales y pulpares

“…Su capacidad de degradar proteínas y modular la respuesta del huésped la hacen una bacteria evolucionada. Autores como Cortelli 24 han podido determinar que esta bacteria no necesita del diente para su presencia, pudiendo identificarla en recién nacidos y niños con dentición mixta, a nivel de lengua, mejilla y surco gingival; así también Topcuoglu 25 , determina que su translocación de zonas de la orofaringe, al oído podrían originar cuadros de infecciosos como otitis media con "derrame". Así también su presencia se intensificaría en pacientes con ciertos cuadros sistémicos, como la Diabetes tipo II, comprobado por Quinteros, 26 el cual determino que diabéticos tipo II con mal control glicémico, presentaban mayor presencia de periodontopatógenos como el.…”