Relationship between olfactory function and olfactory neuronal population in C57BL6 mice injected intraperitoneally with 3‐methylindole

Kim, Jeong Whun; Hong, Sung Lyong; Lee, Chul Hee; Jeon, Eun Jeong; Choi, Ah Reum

doi:10.1016/j.otohns.2010.08.016

Cited by 21 publications

(25 citation statements)

References 29 publications

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“…Olfactory marker protein (OMP), a gene that is expressed almost exclusively on mature primary olfactory sensory neurons [55], was highly down-regulated (11-fold with p*<10 −13 ). Mice that lack OMP have perturbed electrophysiological activity and altered spatiotemporal activity patterns for different odorants [28], [56].…”

Section: Resultsmentioning

confidence: 99%

Dysregulation of Gene Expression in a Lysosomal Storage Disease Varies between Brain Regions Implicating Unexpected Mechanisms of Neuropathology

et al. 2012

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The characteristic neurological feature of many neurogenetic diseases is intellectual disability. Although specific neuropathological features have been described, the mechanisms by which specific gene defects lead to cognitive impairment remain obscure. To gain insight into abnormal functions occurring secondary to a single gene defect, whole transcriptome analysis was used to identify molecular and cellular pathways that are dysregulated in the brain in a mouse model of a lysosomal storage disorder (LSD) (mucopolysaccharidosis [MPS] VII). We assayed multiple anatomical regions separately, in a large cohort of normal and diseased mice, which greatly increased the number of significant changes that could be detected compared to past studies in LSD models. We found that patterns of aberrant gene expression and involvement of multiple molecular and cellular systems varied significantly between brain regions. A number of changes revealed unexpected system and process alterations, such as up-regulation of the immune system with few inflammatory changes (a significant difference from the closely related MPS IIIb model), down-regulation of major oligodendrocyte genes even though white matter changes are not a feature histopathologically, and a plethora of developmental gene changes. The involvement of multiple neural systems indicates that the mechanisms of neuropathology in this type of disease are much broader than previously appreciated. In addition, the variation in gene dysregulation between brain regions indicates that different neuropathologic mechanisms may predominate within different regions of a diseased brain caused by a single gene mutation.

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Section: Resultsmentioning

confidence: 99%

Dysregulation of Gene Expression in a Lysosomal Storage Disease Varies between Brain Regions Implicating Unexpected Mechanisms of Neuropathology

et al. 2012

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“…3‐MI, frequently preferred to induce anosmia when administered intraperitoneally, causes selective dose‐dependent degeneration in the olfactory mucosa after getting into the systemic circulation. It shows its olfactotoxic effect with a 300 or 400 μg/g dose by causing severe hyposmia or anosmia . Food‐finding test is a valuable method in the formation of anosmia and follow‐up of treatment .…”

Section: Discussionmentioning

confidence: 99%

“…The mice were randomly divided into two groups of eight, and each mouse was given a number. Anosmia was induced via intraperitoneal injection of 300 μg/g 3‐methylindole (3‐MI) in 1 mL saline . A week later, the FFT was used to determine whether the animals had developed anosmia.…”

Section: Methodsmentioning

confidence: 99%

The effectiveness of platelet‐rich plasma in an anosmia‐induced mice model

Yasak

Yiğit²,

Server³

et al. 2017

The Laryngoscope

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“…Intraperitoneal administration of 3-methylindole (3-MI) causes a disruption of the OE and olfactory function that continues for at least three weeks21. Two weeks after 3-MI administration, we assessed the effects of linalool vapour exposure using the formalin test.…”

Section: Resultsmentioning

confidence: 99%

“…For olfactory epithelium deprivation, we treated mice with 3-MI, which induces extensive destruction of the olfactory mucosa, resulting in anosmia21. Briefly, 300 mg/kg of 3% 3-MI in corn oil was administered by intraperitoneal injection.…”

Section: Methodsmentioning

confidence: 99%

Odour-induced analgesia mediated by hypothalamic orexin neurons in mice

Tashiro

Yamaguchi

Ishikawa

et al. 2016

Sci Rep

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Various folk remedies employ certain odorous compounds with analgesic effects. In fact, linalool, a monoterpene alcohol found in lavender extracts, has been found to attenuate pain responses via subcutaneous, intraperitoneal, intrathecal, and oral administration. However, the analgesic effects of odorous compounds mediated by olfaction have not been thoroughly examined. We performed behavioural pain tests under odourant vapour exposure in mice. Among six odourant molecules examined, linalool significantly increased the pain threshold and attenuated pain behaviours. Olfactory bulb or epithelium lesion removed these effects, indicating that olfactory sensory input triggered the effects. Furthermore, immunohistochemical analysis revealed that linalool activated hypothalamic orexin neurons, one of the key mediators for pain processing. Formalin tests in orexin neuron-ablated and orexin peptide-deficient mice showed orexinergic transmission was essential for linalool odour-induced analgesia. Together, these findings reveal central analgesic circuits triggered by olfactory input in the mammalian brain and support a potential therapeutic approach for treating pain with linalool odour stimulation.

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Relationship between olfactory function and olfactory neuronal population in C57BL6 mice injected intraperitoneally with 3‐methylindole

Abstract: Our study showed that olfactory impairment was correlated with olfactory neuronal population in mice treated with 3-methylindole. The food-finding test would be a useful tool that could be easily performed without special training in the 3-methylindole-treated C57BL6 anosmic mouse model.

Cited by 21 publications

References 29 publications

Dysregulation of Gene Expression in a Lysosomal Storage Disease Varies between Brain Regions Implicating Unexpected Mechanisms of Neuropathology

Dysregulation of Gene Expression in a Lysosomal Storage Disease Varies between Brain Regions Implicating Unexpected Mechanisms of Neuropathology

The effectiveness of platelet‐rich plasma in an anosmia‐induced mice model

Odour-induced analgesia mediated by hypothalamic orexin neurons in mice

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