Relating click-evoked auditory brainstem response waveforms to hearing loss in the bottlenose dolphin (Tursiops truncatus)

Abstract

Hearing sensitivity in captive Atlantic bottlenose dolphins was assessed using a portable electrophysiologic data collection system, a transducer attached to the pan region of the mandible, and non-invasive recording electrodes. The auditory steady-state response (ASSR) was evoked using sinusoidal amplitude-modulated tones at half octave steps from 20–160 kHz and utilized to determine the upper frequency limit of hearing (i.e., the frequency at which threshold was ≤ 120 dB re 1 μPa). An auditory brainstem response (ABR) was then recorded to a moderate-amplitude click (peak-equivalent sound pressure level of 122 dB re 1 μPa) and examined to determine if relationships existed between the upper frequency limit of hearing and the waveform characteristics of the click-evoked ABR. The ASSR and click-evoked ABR were measured in 6 bottlenose dolphins with varying hearing sensitivity and frequency range of hearing. A significant relationship existed between click-evoked ABR wave amplitudes and the upper frequency limit of hearing. Test times for assessment using frequency-specific ASSR and click-evoked ABR were ∼45 minutes and 1 minute, respectively. With further definition of normative data, measurement of click-evoked ABRs could form the basis of an expedited electrophysiologic method for hearing screening in marine mammals.