Regulatory dephosphorylation of CDK at G2/M in plants: yeast mitotic phosphatase cdc25 induces cytokinin-like effects in transgenic tobacco morphogenesis

Abstract

The data gained using the plants expressing yeast mitotic activator, Spcdc25, clearly argue for the existence and importance of activatory dephosphorylation at G₂/M transition and its interaction with cytokinin signalling in plants. The observed cytokinin-like effects of Spcdc25 expression are consistent with the concept of interaction between cell cycle regulators and phytohormones during plant development. The G₂/M control of the plant cell cycle, however, remains an elusive issue as doubts persist about the mode of activatory dephosphorylation, which in other eukaryotes is provided by Cdc25 phosphatase serving as a final all-or-nothing mitosis regulator.

Discovery of a new intravacuolar protein required for the autophagy, development and virulence of Beauveria bassiana

“…The two control strains showed similar cell cycles with the S phase disappeared on day 5. Intriguingly, four of six cell cycle‐required genes (Spellman et al ., ; Futcher, ; Lipavská et al ., ) were transcriptionally repressed by 57–68% in Δ vlp4 vs. the control strains despite little transcript changes in two other genes. These data demonstrated a linkage of blocked blastospore formation with altered cell cycle in Δ vlp4 .…”

Transcriptional control of fungal cell cycle and cellular events by Fkh2, a forkhead transcription factor in an insect pathogen

“…In addition to the altered cell cycle and septation, four of six genes ( ACE2 , CDC5 , CDC15 , CDC25 , CLB2 and SWI5 ) required for the critical G 2 /M transition 33 34 35 were down-regulated by more than 60% in Δ Fkh2 ( Fig. 2B ).…”

The RING Finger Protein NtRCP1 Is Involved in the Floral Transition in Tobacco (Nicotiana tabacum)

“…As no ortholog of yeast CDC25 protein was identified in higher plants (Doonan and Kitsios, 2009;Boruc et al, 2010), Lipavská et al, (2011) ectopically expressed the yeast Spcdc25 gene in tobacco to better understand the regulation of the G2/M transition in the plant cell cycle. Spcdc25-overexpressing plants exhibited various phenotypic changes, such as early flowering, altered SAM structure and the formation of a higher number of smaller mitotic cells due to a shortened G2 phase in the cell cycle (Lipavská et al, 2011;Vojvodová et al, 2013). Interestingly, these phenotypes were also exhibited by plants overexpressing NtRCP1 in the current study.…”