Regulation of vascular smooth muscle cell autophagy by DNA nanotube-conjugated mTOR siRNA

You, Zaichun; Qian, Hang; Wang, Changzheng; He, Binfeng; Yan, Jiawei; Mao, Chengde; Wang, Guansong

doi:10.1016/j.biomaterials.2015.07.015

Cited by 41 publications

(49 citation statements)

References 80 publications

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“…It was reported that the cellular uptake of DNA origami structures with a size of dozens of nanometers requires more than 12 hours, 3,34 while smaller DNA structures (<20 nm) need 2 to 6 hours. 7,33 In our study, the uptake process is therefore comparatively very fast. Our microplate quantification shows that the relative fluorescence intensities reached a maximum of around three fold higher compared to that of the control within a brief 30 min incubation (Fig.…”

Section: Resultsmentioning

confidence: 53%

“…Although several reports showed that DNA nanostructures carrying siRNA 6,7 or peptides 8,9 had been successfully delivered into cells and taken effect, the details, such as the extracellular and intracellular stability of the DNA nanostructures, cellular uptake mechanisms, and the endosomal escape and cargo release, still remain largely unclear. Thus, elucidating such issues based on current DNA shuriken design is crucial for the future design of more effective, targeted and sophisticated therapeutic DNA nanostructure platforms.…”

Section: Resultsmentioning

confidence: 99%

“…These capabilities have spawned many interesting strategies for designing and constructing structures out of DNA; however, there has not been a deluge of applications arising from these interesting structures. There are pockets of bioapplications of these fascinating structures as nanosized carriers of drugs, 1–5 siRNA, 6,7 peptides 8,9 and in- and out-of-cell sensors. 10–14 This window clearly presents an emerging area for the applications of DNA nanostructures in such fields.…”

Section: Introductionmentioning

confidence: 99%

“…This lack of tools to protect exogenous miR-145 has prompted the imperfect solution of using plasmid or viral vectors to overexpress miR-145 in cancer cells; this strategy has limited real efficacy in clinical settings due to collateral damage to non-cancer cells, lower than 100% uptake by the target cancer cells and an overly complicated multi-stage process. Non-viral DNA nanostructures have instead shown some degree of success in the delivery of other molecular cargo like siRNA, 6,7 aptamers, 26–28 and proteins, 29,30 into cells. While the mechanisms are unclear, DNA nanostructures showed the potential to protect their cargo 11 in harsh degradative conditions.…”

Section: Introductionmentioning

confidence: 99%

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Protecting microRNAs from RNase degradation with steric DNA nanostructures

et al. 2017

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Section: Resultsmentioning

confidence: 53%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protecting microRNAs from RNase degradation with steric DNA nanostructures

et al. 2017

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“…However, the localization of them in cells, after the transfection process, should be also carefully considered. According to one report, 312 the siRNA targeting mammalian target of rapamycin (a regulator protein of autophagy), loaded in DNA nanotubes (13.9 nm length and 6 nm diameter), was effectively transfected into pulmonary arterial smooth muscle cells via endocytosis. The loaded siRNA induced obvious autophagy and inhibited cell growth under both normal and hypoxic conditions.…”

mentioning

confidence: 99%

Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics

Komiyama

Yoshimoto

Sisido

et al. 2017

Bulletin of the Chemical Society of Japan

275

131

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In this review, we introduce two kinds of bio-related nanoarchitectonics, DNA nanoarchitectonics and cellmacromolecular nanoarchitectonics, both of which are basically controlled by chemical strategies. The former DNA-based approach would represent the precise nature of the nanoarchitectonics based on the strict or "digital" molecular recognition between nucleic bases. This part includes functionalization of single DNAs by chemical means, modification of the main-chain or side-chain bases to achieve stronger DNA binding, DNA aptamers and DNAzymes. It also includes programmable assemblies of DNAs (DNA Origami) and their applications for delivery of drugs to target sites in vivo, sensing in vivo, and selective labeling of biomaterials in cells and in animals. In contrast to the digital molecular recognition between nucleic bases, cell membrane assemblies and their interaction with macromolecules are achieved through rather generic and "analog" interactions such as hydrophobic effects and electrostatic forces. This cell-macromolecular nanoarchitectonics is discussed in the latter part of this review. This part includes bottom-up and top-down approaches for constructing highly organized cell-architectures with macromolecules, for regulating cell adhesion pattern and their functions in twodimension, for generating three-dimensional cell architectures on micro-patterned surfaces, and for building synthetic/natural macromolecular modified hybrid biointerfaces.

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The Growing Development of DNA Nanostructures for Potential Healthcare‐Related Applications

Mathur

Medintz

2019

Adv Healthcare Materials

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DNA self‐assembly has proven to be a highly versatile tool for engineering complex and dynamic biocompatible nanostructures from the bottom up with a wide range of potential bioapplications currently being pursued. Primary among these is healthcare, with the goal of developing diagnostic, imaging, and drug delivery devices along with combinatorial theranostic devices. The path to understanding a role for DNA nanotechnology in biomedical sciences is being approached carefully and systematically, starting from analyzing the stability and immune‐stimulatory properties of DNA nanostructures in physiological conditions, to estimating their accessibility and application inside cellular and model animal systems. Much remains to be uncovered but the field continues to show promising results toward developing useful biomedical devices. This review discusses some aspects of DNA nanotechnology that makes it a favorable ingredient for creating nanoscale research and biomedical devices and looks at experiments undertaken to determine its stability in vivo. This is presented in conjugation with examples of state‐of‐the‐art developments in biomolecular sensing, imaging, and drug delivery. Finally, some of the major challenges that warrant the attention of the scientific community are highlighted, in order to advance the field into clinically relevant applications.

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Regulation of vascular smooth muscle cell autophagy by DNA nanotube-conjugated mTOR siRNA

Cited by 41 publications

References 80 publications

Protecting microRNAs from RNase degradation with steric DNA nanostructures

Protecting microRNAs from RNase degradation with steric DNA nanostructures

Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics

The Growing Development of DNA Nanostructures for Potential Healthcare‐Related Applications

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