Regulation of lipid accumulation using nitrogen for microalgae lipid production in Schizochytrium sp. ABC101

Ju, Jung-Hyun; Ko, Dongjin; Heo, Sun-Yeon; Lee, Jong-Jea; Kim, Young‐Min; Lee, Bongsoo; Kim, Min‐Soo; Kim, Chul‐Ho; Seo, Jeong-Woo; Oh, Beak-Rock

doi:10.1016/j.renene.2020.02.047

Cited by 36 publications

(17 citation statements)

References 49 publications

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“…As the mobile phase, 2.5 mM H 2 SO 4 was used, and the flow rate was 0.6 mL/min. The temperatures of the column oven and the RID cell were 65 • C and 45 • C, respectively [40].…”

Section: Cultivation Metabolite Analysismentioning

confidence: 99%

The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability

Jeon

Lee

et al. 2021

Catalysts

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3-Hydroxypropionaldehyde (3-HPA, reuterin) is a broad-spectrum natural antimicrobial agent used in the food industry and other fields. The low yield from the industrial production of 3-HPA using Lactobacillus reuteri and the spontaneous conversion of 3-HPA to acrolein have limited its more widespread use. We isolated L. reuteri BR201 as a biocatalyst for 3-HPA production and confirmed the effect of each factor in the two-step procedure for 3-HPA bioconversion. After initial cultivation for 8 h (late exponential phase), this isolate produced 378 mM of 3-HPA in 1 h at a concentration of OD600 nm 100, 30 °C, and an initial glycerol concentration of 500 mM. This is the highest reported biocatalytic yield of 3-HPA from a glycerol aqueous solution without additives. We confirmed that 4 mM of 3-HPA had antimicrobial activity against five pathogens. The degradation of 3-HPA to acrolein was greater at high temperatures, and there was little degradation when 3-HPA was maintained at 4 °C for 4 weeks. Our results may be useful for future applications of 3-HPA.

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“…As the mobile phase, 2.5 mM H 2 SO 4 was used, and the flow rate was 0.6 mL/min. The temperatures of the column oven and the RID cell were 65 • C and 45 • C, respectively [40].…”

Section: Cultivation Metabolite Analysismentioning

confidence: 99%

The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability

Jeon

Lee

et al. 2021

Catalysts

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“…The DHA as substrate used in the reaction was obtained from Schizochytrium sp. SH103 14 , 16 . The reaction mixture was prepared so as to contain 50 μM substrate in 50 mM Tris–HCl buffer (pH 8.0), and the reaction was initiated by adding Osc-LOX at concentrations of 50–400 units mL −1 .…”

Section: Methodsmentioning

confidence: 99%

Synthesis of two new lipid mediators from docosahexaenoic acid by combinatorial catalysis involving enzymatic and chemical reaction

Heo

et al. 2020

Sci Rep

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Omega-3 polyunsaturated fatty acids (PUFAs) have been known to have beneficial effects in the prevention of various diseases. Recently, it was identified that the bioactivities of omega-3 are related to lipid mediators, called pro-resolving lipid mediators (SPMs), converted from PUFAs, so they have attracted much attention as potential pharmaceutical targets. Here, we aimed to build an efficient production system composed of enzymatic and chemical catalysis that converts docosahexaenoic acid (DHA) into lipid mediators. The cyanobacterial lipoxygenase, named Osc-LOX, was identified and characterized, and the binding poses of enzyme and substrates were predicted by ligand docking simulation. DHA was converted into three lipid mediators, a 17S-hydroxy-DHA, a 7S,17S-dihydroxy-DHA (RvD5), and a 7S,15R-dihydroxy-16S,17S-epoxy-DPA (new type), by an enzymatic reaction and deoxygenation. Also, two lipid mediators, 7S,15R,16S,17S-tetrahydroxy-DPA (new type) and 7S,16R,17S-trihydroxy-DHA (RvD2), were generated from 7S,15R-dihydroxy-16S,17S-epoxy-DPA by a chemical reaction. Our study suggests that discovering new enzymes that have not been functionally characterized would be a powerful strategy for producing various lipid mediators. Also, this combination catalysis approach including biological and chemical reactions could be an effective production system for the manufacturing lipid mediators.

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“…It is thought that the evaluation of this algae for use in areas such as food and feed will make an important contribution. Ju et al (2020) in their study the resulting microalgal lipids are mainly docosahexaenoic acid (DHA) and palmitic acid, also they reported palmitic acid is a saturated fatty acid (SFA) that is used as an emollient and diluent in the cosmetics industry. Fedorova-Dahms et al (2011) reported that Schizochytrium sp.…”

Section: Fatty Acidsmentioning

confidence: 99%

“…They can be produced year-round, depending on the climate and solar radiation, grow at very high rates, and in a wide variety of water sources (fresh, brackish water, seawater and wastewater) (Souza Silva et al, 2014). They can also be easily harvested and can be cultivated under various conditions (Ju et al, 2020). Therefore, the microalgae have an extraordinary biotechnological potential as an alternative to non-renewable resources due to production of natural substances and biomaterials that can be used in various industrial applications (Neto et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Adequate DHA intake protects against many noncommunicable diseases such as diabetes, cardiovascular and neurodegenerative diseases, cancer and schizophrenia (Wang et al, 2020). Microalgal lipids can be produced safely and stably using pure cultures, and the obtained products have potential to use in high-value-added industries, such as medicine and cosmetics (Ju et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Effect of different cell disruption methods on lipid yield of Schizochytrium sp.

İsa

Metin

Ercan³

et al. 2021

Preprint

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In this study, it was investigated to increase the lipid yield of the microalgae Schizochytrium sp., by applying different cell disruption methods. Therefore, acid treatment with HCl, osmotic shock, enzyme applications and ultrasonic homogenizer were tried in this algae species combined with the Bligh and Dyer and Soxhlet methods as an alternative to classical lipid extraction methods. As a result of the study, the highest lipid value (21.72 ± 0.74%) was obtained in enzyme application with Bligh and Dyer method (BDE). The cell disruption processes increased the lipid yield compared to the control groups. The highest PUFA DHA was found in the range of 4.58 ± 2.44-19.25 ± 0.09%, and the highest value was observed in the BDE group. Highest SFA was palmitic acid. Effective results were observed in the Bligh and Dyer applied groups in terms of both total lipid and total fatty acids. In cell disruption methods, particularly in enzyme and HCl extraction, good results were obtained in terms of fatty acids. The highest total fatty acids and the highest lipid content were detected in the Bligh and Dyer enzyme (BDE). Enzyme applications are also advantageous because of being environmentally friendly. Lipid health indices such as n-6/n-3, PUFA/SFA, Atherogenicity index (AI), Thrombogenicity index (TI) and hypocholesterolemic/hypercholesterolemic ratios (HH) were almost favorable. With this study, an appropriate lipid extraction methods were determined to provide an economical and environmental friendly suggestion for future studies to be used in areas such as food, feed and cosmetics.

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Regulation of lipid accumulation using nitrogen for microalgae lipid production in Schizochytrium sp. ABC101

Cited by 36 publications

References 49 publications

The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability

The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability

Synthesis of two new lipid mediators from docosahexaenoic acid by combinatorial catalysis involving enzymatic and chemical reaction

Effect of different cell disruption methods on lipid yield of Schizochytrium sp.

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