Regulation of intestinal calcium absorption by luminal calcium content: Role of intestinal alkaline phosphatase

Brun, L.R.; Brance, María Lorena; Lombarte, Mercedes; Lupo, Maela; Loreto, Veronica Di; Rigalli, Alfredo

doi:10.1002/mnfr.201300686

Cited by 18 publications

(23 citation statements)

References 20 publications

(38 reference statements)

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“…The cells' suspensions were spun down at 750 g for 1 min, resuspended in 1 M NaOH, and radioactivity ( 45 Ca) was measured using a scintillation counter (Beckman LS 100C, USA). Higher radioactivity indicates higher Ca uptake [28].…”

Section: In Vitro Calcium Uptakementioning

confidence: 99%

Effects of Yerba Mate (IIex paraguariensis) on Histomorphometry, Biomechanics, and Densitometry on Bones in the Rat

et al. 2015

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Yerba mate (Ilex paraguariensis) is a xanthine-containing beverage, which is also rich in caffeine. Because caffeine has a negative impact on bone mineral density (BMD) mainly associated with low calcium (Ca) diets, there would be expected a negative effect of yerba mate on bone. In this study, Sprague-Dawley rats were used and randomly assigned into four groups (n = 6/group): Control + Ca 0.2 g %; Control + Ca 0.9 g %; Yerba + Ca 0.2 g %; Yerba + Ca 0.9 g %. At the end of the experiment, tibias and femurs were obtained for BMD, morphometric, histomorphometric, and biomechanical analyses. While there was no difference in bone parameters between rats with and without yerba mate consumption, a negative effect of low Ca diet was observed in BMD, morphometric, histomorphometric, and biomechanical results. Interaction between Ca content in the diet and yerba mate was only found in trabecular bone volume, which would indicate that the negative effect of low Ca intake on bone volume is reversed in part by yerba mate infusion. However, yerba mate was not able to reverse the negative effect of low Ca content on biomechanical properties and trabecular connectivity. In summary, at least in our study, yerba mate would not have a negative effect on bone and would be safe for the bone health of consumers.

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Section: In Vitro Calcium Uptakementioning

confidence: 99%

Effects of Yerba Mate (IIex paraguariensis) on Histomorphometry, Biomechanics, and Densitometry on Bones in the Rat

et al. 2015

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“…In humans, net Ca absorption is not higher than 500–600 mg/day, even when Ca intake goes above 1000 mg/day [2]. A similar situation has been observed in rats [3], and a minute-to-minute regulatory mechanism of Ca uptake dependent of Ca luminal concentration by intestinal alkaline phosphatase (IAP) activity has been proposed [4]. …”

Section: Introductionmentioning

confidence: 85%

“…Previous studies have proposed that IAP acts in the regulatory mechanism of intestinal Ca uptake [3, 4]. In vitro and in vivo experiments using the IAP inhibitor L-phenyalanine (Phe) demonstrated an increase in Ca absorption [3, 4].…”

Section: Discussionmentioning

confidence: 99%

“…This fact is more evident in rats that are fed with a low-Ca diet [3]. Also, it has been demonstrated that IAP modifies the luminal pH as a function of enzyme concentration and Ca luminal content [4]. In addition, a decrease in pH occurred simultaneously with a decrease in Ca absorption [4].…”

Section: Introductionmentioning

confidence: 99%

“…Also, it has been demonstrated that IAP modifies the luminal pH as a function of enzyme concentration and Ca luminal content [4]. In addition, a decrease in pH occurred simultaneously with a decrease in Ca absorption [4]. This decrease in pH mediated by IAP activity as a function of Ca luminal content would inhibit the TRPV6 channels [13–15].…”

Section: Introductionmentioning

confidence: 99%

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Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice

et al. 2017

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Previous studies have demonstrated a negative correlation between intestinal alkaline phosphatase (IAP) activity and calcium (Ca) absorption in the gut, as IAP acts as a protective mechanism inhibiting high Ca entry into enterocytes, preventing Ca overload. Here we evaluated Ca absorption and bone properties in knockout mice (KO) completely devoid of duodenal IAP (Akp3 mice). Female C57BL/6 control mice (WT, n = 7) and KO mice (n = 10) were used to determine Ca absorption in vivo and by in situ isolated duodenal loops followed by histomorphometric analysis of duodenal villi and crypts. Bone mineral density, morphometry, histomorphometry and trabecular connectivity and biomechanical properties were measured on bones. We observed mild atrophy of the villi with lower absorption surface and a significantly higher Ca uptake in KO mice. While no changes were seen in cortical bone, we found better trabecular connectivity and biomechanical properties in the femurs of KO mice compared to WT mice. Our data indicate that IAP KO mice display higher intestinal Ca uptake, which over time appears to correlate with a positive effect on the biomechanical properties of trabecular bone.

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Association between the gut microbiota and mineral metabolism

2017

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The aim of this review is to present the most recent scientific evidence of interactions between the intestinal microbiota and minerals, and the effect of this interaction on the health of the host. The Web of Science database from the years 2013-2017 on this topic was reviewed. Numerous in vitro studies have shown that iron significantly affects the intestinal microbiota. However, Bifidobacteriaceae are capable of binding iron in the large intestine, thereby limiting the formation of free radicals synthesized in the presence of iron, and thus reducing the risk of colorectal cancer. Animal studies have revealed that supplementation with probiotics, prebiotics and synbiotics has a significant effect on bone calcium, phosphate and bone metabolism. The dynamic interaction between microbiota and zinc was shown. Human studies have provided evidence of the influence of probiotic bacteria on parathormone, calcium and phosphate levels and thus on bone resorption. Recent studies have produced new information mainly on the impact of the intestinal bacteria on the metabolism of calcium and iron. From a scientific perspective, the most urgent fields that remain to be investigated are the identification of all human gut microbes and new therapies targeting the interaction between intestinal bacteria and minerals. © 2017 Society of Chemical Industry.

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Regulation of intestinal calcium absorption by luminal calcium content: Role of intestinal alkaline phosphatase

Abstract: Changes in luminal pH arising from intestinal alkaline phosphatase activity induced by luminal calcium concentration modulate intestinal calcium absorption.

Cited by 18 publications

References 20 publications

Effects of Yerba Mate (IIex paraguariensis) on Histomorphometry, Biomechanics, and Densitometry on Bones in the Rat

Effects of Yerba Mate (IIex paraguariensis) on Histomorphometry, Biomechanics, and Densitometry on Bones in the Rat

Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice

Association between the gut microbiota and mineral metabolism

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