Regulation of heme oxygenase-1 expression and MAPK pathways in response to kaempferol and rhamnocitrin in PC12 cells

Hong, Jing-Ting; Yen, Jui-Hung; Wang, Lisu; Lo, Ya-Hsuan; Chen, Zong‐Tsi; Wu, Ming-Jiuan

doi:10.1016/j.taap.2009.02.014

Cited by 79 publications

(43 citation statements)

References 61 publications

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“…In addition, evidence shows that MAPKs are highly expressed in postmitotic neurons of the adult nervous system and are necessary for maintaining normal nervous functions (Hong et al, 2009). MAPKs are activated in response to growth factor stimulation of cells in culture, and fall into four different subgroups: the ERK1/2 MAPK, the Jun N-terminal kinases (JNKs) MAPK, the p38 MAPK and the ERK5 MAPK (Plotnikov, Zehorai, Procaccia, & Seger, 2011).…”

Section: Discussionmentioning

confidence: 99%

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In vivo investigation on the potential of galangin, kaempferol and myricetin for protection of d-galactose-induced cognitive impairment

et al. 2012

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Section: Discussionmentioning

confidence: 99%

“…Kaempferol protects PC12 cells against the oxidative stress induced by H 2 O 2 (Hong et al, 2009). Plants rich in myricetin also show potential neuroprotective effects (Yao & Vieira, 2007).…”

Section: Introductionmentioning

confidence: 99%

In vivo investigation on the potential of galangin, kaempferol and myricetin for protection of d-galactose-induced cognitive impairment

et al. 2012

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“…Furthermore, another study also using PC12 cells (9) showed that kaempferol, a natural flavonoid, and rhamnocitrin, a compound found in various plants, can augment cellular antioxidant defense capacity, at least in part, through regulation of HO-1 expression and MAPK signal transduction. We thus examined at first the pERK1/2 levels in NS/PCs by Western blotting when the cells were cultured for various times (0, 15, 30, 45, and 60 min) in medium containing 10 μM 4MC.…”

Section: Induction Of Ho-1 Expression By 4mcmentioning

confidence: 99%

4-Methylcatechol-induced heme oxygenase-1 exerts a protective effect against oxidative stress in cultured neural stem/progenitor cells via PI3 kinase/Akt pathway

et al. 2010

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ABSTRACT4-Methylcatechol (4MC), a stimulator of the synthesis of neurotrophin family members in various cells, was able to up-regulate the expression of heme oxygenase (HO)-1, a redox-sensitive inducible stress protein, in neural stem/progenitor cells (NS/PCs). RT-PCR analysis showed that 4MC induced HO-1 mRNA expression in a dose-and a time-dependent manner. The increase in HO-1 mRNA was followed by an increase in HO-1 protein content, which was confirmed by ELISA and Western blotting analysis. When NS/PCs were pretreated with 4MC before exposure to hydrogen peroxide (H 2 O 2 ), most of the cells were rescued from the H 2 O 2 -induced death. 4MC enhanced the phosphorylation of mitogen-activated protein kinase (MAPK)/extracellular signal-regulated protein kinase (ERK) and Akt in a time-dependent manner. Pretreatment of cultures with a selective inhibitor of PI3 kinase (PI3K)/Akt, but not with one of MAPK/ERK, inhibited both the 4MC-induced HO-1 expression and neuroprotective effect, demonstrating that PI3K/Akt signaling pathway played a significant role in 4MC-induced HO-1 induction and neuroprotection. Taken together, our results suggest that 4MC activates the expression of HO-1 through the PI3K/Akt signaling pathway and that the HO-1 protein inhibits the death of NS/PCs induced by oxidative stress.

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“…K plays a highly discriminative role over normal and malignant cells, whereby it induces apoptosis in cancer cells only, while leaving healthy cells unaffected. [13][14][15] A is a notable flavone, highly present in several dietary plants such as parsley, celery, onions, lemon balm, and oranges. 16 Comprehensive study reports revealed its potential antioxidant, anti-inflammatory, and anticarcinogenic properties, apparently through its interactions with the signaling molecules in the three major biopathways, that is, ERK, JNK, and p38 in both in vitro and in vivo models.…”

Section: Introductionmentioning

confidence: 99%

Optimization, formulation, and characterization of multiflavonoids-loaded flavanosome by bulk or sequential technique

Karthivashan¹,

Kura²,

Abas³

et al. 2016

IJN

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This study involves adaptation of bulk or sequential technique to load multiple flavonoids in a single phytosome, which can be termed as “flavonosome”. Three widely established and therapeutically valuable flavonoids, such as quercetin (Q), kaempferol (K), and apigenin (A), were quantified in the ethyl acetate fraction of Moringa oleifera leaves extract and were commercially obtained and incorporated in a single flavonosome (QKA–phosphatidylcholine) through four different methods of synthesis – bulk (M1) and serialized (M2) co-sonication and bulk (M3) and sequential (M4) co-loading. The study also established an optimal formulation method based on screening the synthesized flavonosomes with respect to their size, charge, polydispersity index, morphology, drug–carrier interaction, antioxidant potential through in vitro 1,1-diphenyl-2-picrylhydrazyl kinetics, and cytotoxicity evaluation against human hepatoma cell line (HepaRG). Furthermore, entrapment and loading efficiency of flavonoids in the optimal flavonosome have been identified. Among the four synthesis methods, sequential loading technique has been optimized as the best method for the synthesis of QKA–phosphatidylcholine flavonosome, which revealed an average diameter of 375.93±33.61 nm, with a zeta potential of −39.07±3.55 mV, and the entrapment efficiency was >98% for all the flavonoids, whereas the drug-loading capacity of Q, K, and A was 31.63%±0.17%, 34.51%±2.07%, and 31.79%±0.01%, respectively. The in vitro 1,1-diphenyl-2-picrylhydrazyl kinetics of the flavonoids indirectly depicts the release kinetic behavior of the flavonoids from the carrier. The QKA-loaded flavonosome had no indication of toxicity toward human hepatoma cell line as shown by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide result, wherein even at the higher concentration of 200 µg/mL, the flavonosomes exert >85% of cell viability. These results suggest that sequential loading technique may be a promising nanodrug delivery system for loading multiflavonoids in a single entity with sustained activity as an antioxidant, hepatoprotective, and hepatosupplement candidate.

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Regulation of heme oxygenase-1 expression and MAPK pathways in response to kaempferol and rhamnocitrin in PC12 cells

Cited by 79 publications

References 61 publications

In vivo investigation on the potential of galangin, kaempferol and myricetin for protection of d-galactose-induced cognitive impairment

In vivo investigation on the potential of galangin, kaempferol and myricetin for protection of d-galactose-induced cognitive impairment

4-Methylcatechol-induced heme oxygenase-1 exerts a protective effect against oxidative stress in cultured neural stem/progenitor cells via PI3 kinase/Akt pathway

Optimization, formulation, and characterization of multiflavonoids-loaded flavanosome by bulk or sequential technique

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