Regulation of Epithelial Cell Functions by the Osmolality and Hydrostatic Pressure Gradients: A Possible Role of the Tight Junction as a Sensor

Tokuda, Shinsaku; Yu, Alan

doi:10.3390/ijms20143513

Cited by 25 publications

(19 citation statements)

References 121 publications

(156 reference statements)

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“…Although AVP is the main controlling hormone of renal water handling [4,49], we could corroborate that local osmolality changes were able to regulate IMCD transport properties and claudin expression independently, similar to the findings of Lanaspa and coworkers [38,39]. Whereas in these studies TJ proteins have been described as targets for regulation, intriguingly, it has also been proposed that the TJ itself could serve as sensor for osmolality and tonicity changes, thereby becoming the origin of regulation (reviewed in [51]). In MDCK cells, acute changes in transepithelial osmotic gradients dramatically changed paracellular selectivity and claudin localization as well as cytoskeleton remodeling [52].…”

Section: Discussionsupporting

confidence: 87%

Claudin-19 Is Regulated by Extracellular Osmolality in Rat Kidney Inner Medullary Collecting Duct Cells

Ziemens

Sonntag

Wulfmeyer

et al. 2019

IJMS

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The inner medullary collecting duct (IMCD) is subject to severe changes in ambient osmolality and must either allow water transport or be able to seal the lumen against a very high osmotic pressure. We postulate that the tight junction protein claudin-19 is expressed in IMCD and that it takes part in epithelial adaptation to changing osmolality at different functional states. Presence of claudin-19 in rat IMCD was investigated by Western blotting and immunofluorescence. Primary cell culture of rat IMCD cells on permeable filter supports was performed under different osmotic culture conditions and after stimulation by antidiuretic hormone (AVP). Electrogenic transepithelial transport properties were measured in Ussing chambers. IMCD cells cultivated at 300 mosm/kg showed high transepithelial resistance, a cation selective paracellular pathway and claudin-19 was mainly located in the tight junction. Treatment by AVP increased cation selectivity but did not alter transepithelial resistance or claudin-19 subcellular localization. In contrast, IMCD cells cultivated at 900 mosm/kg had low transepithelial resistance, anion selectivity, and claudin-19 was relocated from the tight junctions to intracellular vesicles. The data shows osmolality-dependent transformation of IMCD epithelium from tight and sodium-transporting to leaky, with claudin-19 expression in the tight junction associated to tightness and cation selectivity under low osmolality.

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Section: Discussionsupporting

confidence: 87%

Claudin-19 Is Regulated by Extracellular Osmolality in Rat Kidney Inner Medullary Collecting Duct Cells

Ziemens

Sonntag

Wulfmeyer

et al. 2019

IJMS

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“…When milk accumulates in the alveolar cavity and when the mechanical pressure of the milk is supported by contraction of myoepithelial cells, the secretory cells of the alveoli are exposed to strong and repeated vectorial forces (Markov, 2001). Mechanosensitivity of murine mammary gland alveoli has been reported previously (Tolkunov and Markov, 1997). These results were confirmed in HC11 mammary gland epithelial cell monolayers when defined hydrostatic pressure was applied (Mießler et al, 2018a, b).…”

Section: Introductionmentioning

confidence: 63%

Accumulation of milk increases the width of tight junctions in the epithelium of mouse mammary alveoli

Круглова

Razgovorova

Amasheh

et al. 2020

BioComm

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The study of the molecular mechanisms of maintaining the integrity of the epithelium during mechanical stress remains a relevant problem in the physiology of tissue barriers. A methodical approach has been applied which makes it possible to reproduce mechanical pressure on the apical region of cells in vivo and to study the participation and role of tight junctions in maintaining the integrity of the epithelial structure. Mammary gland tissue specimens from lactating control mice and animals after a 20-h interruption of suckling were prepared and the width of the tight junction of the secretory epithelium was analyzed. At the ultrastructural level, it was shown that accumulation of milk caused a significant increase in the width of the tight junction between epithelial cells. In the control group, the width of this structure was 2.1 ± 0.1 µm; in the experimental group — 4.2 ± 0.1 μm. The marked increase in the width of tight junctions between epithelial cells is in accordance with an observed increase in the level of claudin-1 and -3 in the secretory epithelium and can be interpreted as adaptive changes aimed at maintaining the structure of the alveoli.

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“…Our findings suggest that gut functional disturbances also belong to the earliest remodeling events induced by simulated microgravity. Epithelium TJs are known as a sensor of osmolality and hydrostatic pressure gradients [ 55 , 56 ]. Thus, HS-induced TER abnormalities ( Figure 5 ) can result from changes in the hydrostatic pressure gradient in the abdominal cavity of hindlimb suspended rats, when radical redistribution of body fluids occurs with possible changes in the water–salt balance.…”

Section: Discussionmentioning

confidence: 99%

Circulating Ouabain Modulates Expression of Claudins in Rat Intestine and Cerebral Blood Vessels

Markov

Fedorova

Кравцова

et al. 2020

IJMS

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The ability of exogenous low ouabain concentrations to affect claudin expression and therefore epithelial barrier properties was demonstrated previously in cultured cell studies. We hypothesized that chronic elevation of circulating ouabain in vivo can affect the expression of claudins and tight junction permeability in different tissues. We tested this hypothesis in rats intraperitoneally injected with ouabain (1 μg/kg) for 4 days. Rat jejunum, colon and brain frontal lobes, which are variable in the expressed claudins and tight junction permeability, were examined. Moreover, the porcine jejunum cell line IPEC-J2 was studied. In IPEC-J2-cells, ouabain (10 nM, 19 days of incubation) stimulated epithelial barrier formation, increased transepithelial resistance and the level of cSrc-kinase activation by phosphorylation, accompanied with an increased expression of claudin-1, -5 and down-regulation of claudin-12; the expression of claudin-3, -4, -8 and tricellulin was not changed. In the jejunum, chronic ouabain increased the expression of claudin-1, -3 and -5 without an effect on claudin-2 and -4 expression. In the colon, only down-regulation of claudin-3 was observed. Chronic ouabain protected the intestine transepithelial resistance against functional injury induced by lipopolysaccharide treatment or by modeled acute microgravity; this regulation was most pronounced in the jejunum. Сlaudin-1 was also up-regulated in cerebral blood vessels. This was associated with reduction of claudin-3 expression while the expression of claudin-5 and occludin was not affected. Altogether, our results confirm that circulating ouabain can functionally and tissue-specifically affect barrier properties of epithelial and endothelial tissues via Na,K-ATPase-mediated modulation of claudins expression.

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Regulation of Epithelial Cell Functions by the Osmolality and Hydrostatic Pressure Gradients: A Possible Role of the Tight Junction as a Sensor

Cited by 25 publications

References 121 publications

Claudin-19 Is Regulated by Extracellular Osmolality in Rat Kidney Inner Medullary Collecting Duct Cells

Claudin-19 Is Regulated by Extracellular Osmolality in Rat Kidney Inner Medullary Collecting Duct Cells

Accumulation of milk increases the width of tight junctions in the epithelium of mouse mammary alveoli

Circulating Ouabain Modulates Expression of Claudins in Rat Intestine and Cerebral Blood Vessels

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