Regulation Of Brn-3a N-terminal transcriptional activity by MEK1/2-ERK1/2 signalling in neural differentiation

Berwick, Daniel C.; Calissano, Mattia; Corness, Jacqueline D.; Cook, Simon J.; Latchman, David S.

doi:10.1016/j.brainres.2008.12.009

Cited by 16 publications

(18 citation statements)

References 41 publications

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“…It has been reported that ERK1/2 (p42/p44) is phosphorylated during the differentiation of neuronal progenitor cells (8). Similarly, when phosphorylation of ERK1/2 was assessed following treatment with BC, a marked increase was observed, whereas the expression of total ERK was unchanged (Fig.…”

Section: Bc-induced Neuronal Cell Differentiation Involves Erk Phosphmentioning

confidence: 59%

“…DLK1 has been known to modulate differentiation signaling in adipocytes and hematopoietic stem cells and has been characterized as a bona fide stem cell gene in neuronal tumors (5,6). Mitogen activated protein kinase (MAPK) signaling pathways have been shown to play pivotal roles in growth, differentiation and stress responses (7,8). It has been reported that MAPK signaling is affected by the expression of DLK1 (9).…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that MAPK signaling is affected by the expression of DLK1 (9). Correspondingly, extracellular signal-regulated kinases (ERK)(p42/44) are phosphorylated during neuronal differentiation and the activation of MEK/ERK signaling was found to decrease the self-renewal capacity of embryonal stem cells (8,10).…”

Section: Introductionmentioning

confidence: 99%

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Effect of β-carotene on cancer cell stemness and differentiation in SK-N-BE(2)C neuroblastoma cells

Lee

Park²,

Kim³

2013

Oncology Reports

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Abstract. Neuroblastoma is a solid tumor often diagnosed in childhood. While there have been intense efforts to develop a treatment for neuroblastoma, current therapies remain unsuccessful due to high rate of resistance and metastasis. Most cancers originate from a subset of self-renewing cells, primarily cancer stem cells (CSCs), which establish a tumor through continuous self-renewal and differentiation. The successful elimination of CSCs is an important goal in the development of effective strategies to achieve complete remission for cancers. Although β-carotene has been associated with several anticancer mechanisms, the efficacy of β-carotene against CSCs remains unclear. In the present study, β-carotene was shown to reduce cell growth and induce neuronal cell differentiation, concomitant with a marked increase in the phosphorylation of extracellular signal-regulated kinases (ERK) (p42/p44). More importantly, β-carotene inhibited self-renewal characteristics of CSCs and decreased expression of several stem cell markers. Levels of mRNA and protein of Drosophila delta-like 1 homolog (Drosophila) (DLK1) were downregulated following treatment with β-carotene. In addition, knockdown of DLK1 by siRNA enhanced the inhibitory effect of β-carotene on colony formation of neuroblastoma cells. β-carotene also potentiated the effect of cisplatin on the self-renewal characteristics of CSCs in neuroblastoma, revealing that β-carotene has the capacity to resensitize cells to cisplatin cytotoxicity by directly targeting CSCs. In conclusion, β-carotene was shown to strongly increase the anticancer efficacy against neuroblastoma cancer stem-like cells. Moreover, these results suggest that the targeting of CSCs is a novel mechanism of β-carotene. Thus, β-carotene is a potential chemotherapeutic reagent for this cancer.

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Section: Bc-induced Neuronal Cell Differentiation Involves Erk Phosphmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

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Effect of β-carotene on cancer cell stemness and differentiation in SK-N-BE(2)C neuroblastoma cells

Lee

Park²,

Kim³

2013

Oncology Reports

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“…The sequences of ARE-SV40 and all intermediates were confirmed by sequencing. LTRpoly and LTR-3a-as, encoding an inverted 217-nucleotide sequence from the region of Brn-3a mRNA encoding amino acids 41-114 of Brn-3aL has been published before (14). pGL2-HSP27, encoding the human HSP27 proximal promoter upstream of luciferase, has been described previously (19).…”

Section: Non-computational Prediction Of Co-complexed Proteins-mentioning

confidence: 99%

“…Presumably grossly impaired in nociception and proprioception, these animals are unable to feed, and they die shortly after birth (9,10). Supporting a key role for Brn-3a in sensory neural development, its overexpression in neuroblastoma cell lines is sufficient to induce differentiation (11)(12)(13)(14). Brn-3a has been shown to up-regulate a number of neural genes, including neurofilaments, snap25, the neuropeptide Galanin, and the sodium channel SCN9A as well as the cell cycle inhibitor P21 (12, 14 -18).…”

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confidence: 99%

A Simple Technique for the Prediction of Interacting Proteins Reveals a Direct Brn-3a-Androgen Receptor Interaction

Berwick

Diss

Budhram-Mahadeo

et al. 2010

Journal of Biological Chemistry

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The formation of multiprotein complexes constitutes a key step in determining the function of any translated gene product. Thus, the elucidation of interacting partners for a protein of interest is of fundamental importance to cell biology. Here we describe a simple methodology for the prediction of novel interactors. We have applied this to the developmental transcription factor Brn-3a to predict and verify a novel interaction between Brn-3a and the androgen receptor (AR). We demonstrate that these transcription factors form complexes within the nucleus of ND7 neuroblastoma cells, while in vitro pull-down assays show direct association. As a functional consequence of the Brn3a-AR interaction, the factors bind cooperatively to multiple elements within the promoter of the voltage-gated sodium channel, Nav1.7, leading to a synergistic increase in its expression. Thus, these data define AR as a direct Brn-3a interactor and verify a simple interacting protein prediction methodology that is likely to be useful for many other proteins.Although the importance of protein-protein interaction is long established, the rise of postgenomic technologies, in particular interactomics in yeast (1), and the growing popularity of systems biology (2) have served to highlight the importance of protein complexes to cell biology. For example, in the field of transcriptional regulation, the discovery of cis-regulatory elements vast distances from the transcriptional start site of a gene requires the formation of protein complexes to bridge often huge expanses of genomic DNA (3). In addition, the concept of promoters and cis-regulatory elements as "coincidence detectors" that integrate and process a diversity of inputs into a decision on the expression of a gene requires the construction of complex molecular machinery to perform these tasks (4). Although the need to study multiprotein complexes in basic research is clear, its relevance to applied disciplines should not be underestimated. In the case of transcriptional complexes, there is a growing body of data supporting the idea that interactions between transcription factors and between transcription factors and co-factors constitute a potent target for therapeutic intervention (5-7). In addition to molecules that block or disrupt protein-protein interactions, the creation of drugs that stabilize a transcriptional complex has been postulated (6), thus suggesting the possibility that, by modulating protein binding events, genes critical to pathological processes may be switched on or off pharmacologically.The Brn-3a transcription factor (also known as Pou4F1) is a class IV POU (Pit-Oct-Unc) family transcription factor. It is expressed at high levels in the sensory neurons of the trigeminal ganglia and dorsal root ganglia (DRG) 2 and in specific structures of the brain (8). Brn-3a is indispensable for normal development because Brn-3a knock-out mice display a severe depletion of sensory neurons. Presumably grossly impaired in nociception and proprioception, these animals are unable to...

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DNA synthesis of rat bone marrow mesenchymal stem cells through α1-adrenergic receptors

Han

Zou

Zhu

et al. 2009

Archives of Biochemistry and Biophysics

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Regulation Of Brn-3a N-terminal transcriptional activity by MEK1/2-ERK1/2 signalling in neural differentiation

Cited by 16 publications

References 41 publications

Effect of β-carotene on cancer cell stemness and differentiation in SK-N-BE(2)C neuroblastoma cells

Effect of β-carotene on cancer cell stemness and differentiation in SK-N-BE(2)C neuroblastoma cells

A Simple Technique for the Prediction of Interacting Proteins Reveals a Direct Brn-3a-Androgen Receptor Interaction

DNA synthesis of rat bone marrow mesenchymal stem cells through α1-adrenergic receptors

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