Regulating cytoskeleton‐based vesicle motility

Abstract

During vesicular transport, the assembly of the coat complexes and the selection of cargo proteins must be coordinated with the subsequent translocation of vesicles from the donor to an acceptor compartment. Here, we review recent progress toward uncovering the molecular mechanisms that connect transport vesicles to the protein machinery responsible for cytoskeleton-mediated motility. An emerging theme is that vesicle cargo proteins, either directly or through binding interactions with coat proteins, are able to influence cytoskeletal dynamics and motor protein function. Hence, a vesicle's cargo composition may help direct its intracellular motility and targeting.

Matrix invasion by tumour cells: a focus on MT1-MMP trafficking to invadopodia

“…Although it is not completely understood, the function of cortactin at invadopodia might involve its capacity to interact with actin filaments and activate Arp2/3-complex-mediated branching (Ayala et al, 2008;Weaver et al, 2001). Cortactin has an important role in membrane traffic, being a regulator of Arp2/3-mediated actin branching, a process that is required for the generation and/or fission of vesicles (Hehnly and Stamnes, 2007). A recent study has brought novel insights to the function of cortactin in invasion by showing that cortactin might regulate secretion of MT1-MMP and MMP-2 and/or MMP-9 at invadopodia .…”

Genetic identification of intracellular trafficking regulators involved in notch dependent binary cell fate acquisition following asymmetric cell division

“…We selected these genes among members of intracellular trafficking regulator families mostly identified from yeast genetics and involved in different trafficking aspects such as, coat components [clathrin mediated endocytosis (Maldonado-Báez and Wendland, 2006)], lipid microdomain organisation [non-clathrin mediated endocytosis (Simons and Gerl, 2010)], cytoskeleton [actin, myosin and/or microtubules (Hehnly and Stamnes, 2007)], small GTPases, ubiquitylation/ deubiquitylation factors involved in vesicle targeting (Murphy et al, 2009;Wennerberg et al, 2005), Endosomal Sorting Complex Required for Transport (ESCRT) complexes (Henne et al, 2011), membrane recognition and/or fusion regulators [such as SNAP receptors, SNAREs (Malsam et al, 2008), Exocyst (Hsu et al, 2004)] and ATPases (Forgac, 2007). We also based our selection on gene ontology (GO) annotation from FlyBase (using the GO terms: endocytosis, endosomal sorting, secretion) and selected putative orthologue(s) of traffic regulators identified in a Caenorhabditis elegans genetic screen (Balklava et al, 2007) or mammal proteomic screens (Baust et al, 2008;Baust et al, 2006).…”

Apaf1 plays a pro-survival role by regulating centrosome morphology and function

“…The distribution and organization of the endoplasmic reticulum (ER) also depends on interactions with microtubules, and one level of regulation of mitochondria (besides fission and fusion) is their active transport along microtubules (Jahani-Asl et al, 2010;Park and Blackstone, 2010). In addition, microtubules are essential for vesicle transport, because vesicles use the microtubule network to meet and fuse with several different endocytic organelles (Hehnly and Stamnes, 2007). Besides their function as organizers of the cytoskeleton and the mitotic spindle, centrosomes serve also as spatiotemporal organizers of events regulating cell cycle, probably by triggering G1 to S transition checkpoints (Rai et al, 2008;Rieder et al, 2001).…”