Regulated serine proteinase lytic system on mammalian sperm surface: There must be a role

Cesari, Andreína; Monclus, Maria; Tejón, Gabriela; Clementi, Marisa; Fornés, Miguel Walter

doi:10.1016/j.theriogenology.2010.03.029

Cited by 29 publications

(41 citation statements)

References 150 publications

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“…The biological role of SPINK13 in the sperm acrosome and how its down-regulation causes the fertility reduction remains to be elucidated. One possibility is that SPINK13 functions as a protease inhibitor necessary for the regulation of critical proteases involved in early signaling events during fertilization, which is consistent with the hypothesis that the regulated serine protease activity might be the key of sperm maturation (8). Furthermore, the fact that SPINK13 is evolutionarily conserved from rodent to human and has no expression in testis indicates that it is a putative target for post-testicular male contraception.…”

Section: Discussionsupporting

confidence: 72%

“…Several protease inhibitors expressed in certain regions are expected to be responsible for this control. They are thought to play a role in capacitation by stabilizing ZP binding sites during uterine passage, followed by dissociation to allow sperm-ZP interaction (8). Hence, our future work will concentrate on the molecular mechanism for this regulation and clarify its related signal networks.…”

Section: Discussionmentioning

confidence: 99%

“…This suggests that the sperm surface architecture depends on correct localization of its components and highlights the importance of the sequential proteolytic processing of the sperm surface proteins in the epididymal duct. Proteolysis in the epididymal lumen thus needs to be well controlled, and several protease inhibitors expressed in the certain epididymal regions are expected to be responsible for this control (3,8).…”

mentioning

confidence: 99%

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Spink13, an Epididymis-specific Gene of the Kazal-type Serine Protease Inhibitor (SPINK) Family, Is Essential for the Acrosomal Integrity and Male Fertility

et al. 2013

Journal of Biological Chemistry

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Background:The molecular mechanism involved in sperm maturation is still not completely elucidated. Results:We identified an epididymis-specific gene, Spink13, that is essential for the acrosomal integrity and sperm maturation. Conclusion: Our findings provided direct evidence of a modulatory effect of SPINK13 on fertilization. Significance: We expect further study of SPINK13 will provide a new putative target for post-testicular male contraceptives.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Spink13, an Epididymis-specific Gene of the Kazal-type Serine Protease Inhibitor (SPINK) Family, Is Essential for the Acrosomal Integrity and Male Fertility

et al. 2013

Journal of Biological Chemistry

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“…Cesari et al [21] suggested that turning off a single enzyme could lead to its replacement by another enzyme with redundant activity on the mammalian sperm surface. A good example is acrosin, which was long believed to participate in the limited proteolysis of ZP and thereby in the successful fusion of the sperm and egg.…”

Section: Discussionmentioning

confidence: 99%

MSG is involved in sperm gelatinolytic activity in the prawn, Macrobrachium rosenbergii

Yang

Qian

et al. 2013

Chin. Sci. Bull.

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Proteases in male reproductive tract are considered to play key roles in fertilization processes. In contrast to mammals, there are limited data concerning crustacean sperm proteases. We previously identified a novel sperm gelatinase (MSG) from Macrobrachium rosenbergii that was inhibited by a male reproduction-related Kazal-type protease inhibitor (MRPINK) specifically. In the present study, MSG was found to be distributed on the vas deferens and terminal ampullae by Western blot. Immunohistochemical analyses revealed that MSG was expressed in secretory epithelial cells and sperm distributed mainly at the base zone. RNA interference (RNAi) mediated knock-down of MSG resulted in a marked loss of sperm gelatinolytic activity. Taken together, the results show that MSG is closely linked to and probably involved in the fertilization process.

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“…Some changes observed in in vivo sperm capacitation are similar to cryo-capacitation and include an increase in plasma membrane permeability, intracellular increase in calcium ion concentrations (Ca2 +) and cyclic adenosine monophosphate (cAMP) and tyrosine phosphorylation (Pommer and Meyers, 2002;Kumar and Atreja, 2011;Singh et al, 2012). In response to these changes, there is destabilization of proteins from the serine protease family present both in the acrosome and the sperm membrane (Cesari et al, 2010), leading to exocytosis of hyaluronidase and acrosin, which are important enzymes that digest the zona pellucida matrix, compromising the penetration of spermatozoa into the oocyte (Adham et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Plasminogen activator inhibitor 1 and Antipain preserve acrosome integrity of bovine spermatozoa during cryopreservation

Branco

Moraes

et al. 2017

Arq. Bras. Med. Vet. Zootec.

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Seminal plasma contains serine proteases and serine protease inhibitor, which are involved in mammalian fertilization, and the inhibitors can be applied to prevent cold-induced sperm capacitation. The effects of different concentrations of two serine protease inhibitors were analyzed, Plasminogen activator inhibitor 1 - PAI-1 (70ƞg, 140ƞg and 210 ƞg) and Antipain (10µg, 50µg and 100µg) as supplementation to bovine semen cryopreservation extender. The effects of the inhibitors on the sperm parameters (sperm kinetics - CASA, acrosome integrity, plasma membrane integrity, mitochondrial membrane potential, sperm defects and acrosome reaction rate) were evaluated in the post-thaw semen. Cryopreservation of sperm with Antipain decreased post-thaw kinetic parameters of MP, VSL, LIN, SRT and the percentage of hyper-activated sperm while PAI-1 (210 ƞg) decreased VSL and LIN. Antipain and PAI-1 had no effect on the integrity parameters of the plasma membrane, mitochondrial membrane potential and sperm defects. Sperm cryopreserved in the presence of Antipain and PAI-1 (70 and 140 ƞg) preserved acrosome integrity, as they were able to complete the in vitro acrosome reaction. In conclusion, the serine protease inhibitors, Antipain and PAI-1 (70 and 140ƞg) are able to preserve the acrosome integrity of cryopreserved bovine sperm.

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Regulated serine proteinase lytic system on mammalian sperm surface: There must be a role

Cited by 29 publications

References 150 publications

Spink13, an Epididymis-specific Gene of the Kazal-type Serine Protease Inhibitor (SPINK) Family, Is Essential for the Acrosomal Integrity and Male Fertility

Spink13, an Epididymis-specific Gene of the Kazal-type Serine Protease Inhibitor (SPINK) Family, Is Essential for the Acrosomal Integrity and Male Fertility

MSG is involved in sperm gelatinolytic activity in the prawn, Macrobrachium rosenbergii

Plasminogen activator inhibitor 1 and Antipain preserve acrosome integrity of bovine spermatozoa during cryopreservation

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