Registration of the Novel Wheat Lines L658, L693, L696, and L699, with Resistance to Fusarium Head Blight, Stripe Rust, and Powdery Mildew

Abstract

Four red spring wheat (Triticum aestivum L.) lines, L658 (Reg. No. GP-971, PI 672537), L693 (Reg. No. GP-972, PI 672538), L696 (Reg. No. GP-973, PI 672539), and L699 (Reg. No. GP-974, PI 672540), were developed at the Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University of China from the cross 'MY11'/YU25, the latter of which was derived from a cross between common wheat (Triticum aestivum, 2n = 6x = 42, AABBDD) and Thinopyrum intermedium (Host) Barkworth & D.R. Dewey (2n = 6x = 42; JJJ s J s SS). The four wheat lines have high levels of resistance to Fusarium head blight (FHB) (mainly caused by Fusarium graminearum Schwabe) similar to those of the FHB-resistant genotypes 'Sumai 3' and 'Wangshuibai' based on results of experiments conducted in three diferent years in three diferent areas. In addition to FHB resistance, these lines also have strong resistance to both stripe rust (caused by Puccinia striiformis Westend. f. sp. tritici), with infection type (IT) = 0 or 0;, and powdery mildew (caused by Erysiphe graminis f. sp. tritici), with IT = 0. The results obtained in the Henan Province of China also show that these lines have suitable agronomic and morphological traits. These results indicate that the novel resistant wheat lines L658, L693, L696, and L699 could play an important role in wheat genetic improvement, especially for FHB, stripe rust, and powdery mildew resistance.

Wheat WCBP1 encodes a putative copper-binding protein involved in stripe rust resistance and inhibition of leaf senescence

“…No. GP-972, PI 672538) is from F 6:7 families of a cross between MY11 and YU25, the latter being a derivative of a wide cross involving Thinopyrum intermedium [ 17 ]. Genetic analysis demonstrated that stripe rust resistance observed in L693 is conferred by a single dominant gene, YrL693 , which was mapped to chromosome 1B by linkage analysis with simple sequence repeat (SSR) markers and Chinese Spring nulli-tetrasomic lines [ 18 , 19 ].…”

Identification of three new resources of resistance to Fusarium head blight in wheat

“…Since the use of Sumai3 in breeding is still unsuccessful (Chen et al 2012), breeders appreciate other germplasm of resistance with some improved and important traits. Previous studies showed that L699 had a strong FHB resistance, which was similar to those of Sumai3 and Wangshuibai (Zhang et al 2011;Liu et al 2015;Yang et al 2016). However, it does not meet the requirement for FHB resistance.…”

“…In China, FHB epidemics have frequently occurred in the middle to lower valleys of the Yangtze River (Yao & Lu 2000). In recent years, FHB has spread to the southwest of China, including Sichuan Province (Zhang et al 2011;Liu et al 2015;Yang et al 2016), where the climate was warm and rainy. Furthermore, FHB is also a fungal disease threatening food and economic security due to its contamination with mycotoxins, decreasing the wheat yield and quality (Zwart et al 2008).…”

Collinearity Analysis and High-Density Genetic Mapping of the Wheat Powdery Mildew Resistance Gene Pm40 in PI 672538

“…From agronomical traits and application standpoints of view, we developed the following four spring wheat lines: L658 (PI 672537), L693 (PI 672538), L696 (PI 672539), and L699 (PI 672540). These lines exhibited excellent agronomic traits, including yield and resistance to Fusarium head blight conferred by FhbL693a and FhbL693b [ 24 ], to stripe rust conferred by YrL693 [ 25 ] and to powdery mildew conferred by Pm40 [ 22 , 23 , 26 , 27 ]. Though the pedigree of PI 672538 includes Th .…”

“…Pm21 still exhibits strong resistance to powdery mildew and is the most commonly employed powdery mildew resistance gene in Chinese breeding programs [ 19 ], but new isolates that are highly virulent to Pm21 have been reported [ 29 , 30 ]. However, Pm40 confers strong resistance to powdery mildew in the field of both Henna and Sichuan Provinces of China [ 21 , 25 – 26 ]), and this gene is effective against nearly all isolates collected from the main wheat growing regions of China [ 23 ]. Thus, Pm40 may be widely used in future Chinese breeding programs as an alternative to Pm21 , necessitating the identification and prioritization of molecular markers closely associated with Pm40 by marker-assisted selection is an important task.…”