Registration of Sheep Brain MR Images for Cell Tracking Using Ferrite-Composite Micro-Beads as Markers

Abstract

In the recent molecular imaging studies of the magnetic resonance imaging field, in-vivo cell tracking is becoming an issue for the observation of cell therapy and disease behavior. In order to perform cell tracking, high resolution images and long-term studies are required, because those images make better performance than low resolution images in the recognition of cells and cells are traced at least two weeks. Image registration is an essential image processing technique for long-term imaging. In this study, we proposed the image registration technique using ferrite-composite micro- beads which could be used as substitutes for the cells labeling MR contrast agent such as superparamagnetic iron oxide (SPIO) nanoparticles. Registration of sheep brain images tagging micro-beads was performed with the sufficient accuracy for cell tracking.

Labelling of mammalian cells for visualisation by MRI

“…Microbeads used for magnetic cell sorting can be used as a contrast agent linked to an antibody that is bound to the target cell [125]. The advantage of this labelling method is that the particles do not internalise, and therefore it is assumed that no toxic effects of the particle are exerted.…”

A Facile Solvothermal Synthesis and Magnetic Properties of MnFe2O4 Spheres with Tunable Sizes

“…In the past decades, MnFe 2 O 4 has received considerable attention for its broad applications in many scientific and technological fields including magnetic storage, electronic device, ferrofluids, catalysts, microwave, biomedicine (e.g., targeted drug delivery, magnetic resonance imaging (MRI), biosensor, as well as cancer diagnosis, and treatment, etc.) . To meet the requirements for these promising applications, MnFe 2 O 4 must have not only suitable magnetic properties (e.g., large saturation magnetization [ M s ], remanent magnetization [ M r ], and moderate coercivity [ H c ]), but also high‐hydrophilic property as well as reduced size and uniform morphology.…”

“…I N the past decades, MnFe 2 O 4 has received considerable attention for its broad applications in many scientific and technological fields including magnetic storage, 1 electronic device, 2 ferrofluids, 3 catalysts, 4,5 microwave, 6 biomedicine (e.g., targeted drug delivery, magnetic resonance imaging (MRI), biosensor, as well as cancer diagnosis, and treatment, etc.). [7][8][9][10][11] To meet the requirements for these promising applications, MnFe 2 O 4 must have not only suitable magnetic properties (e.g., large saturation magnetization [M s ], remanent magnetization [M r ], and moderate coercivity [H c ]), but also high-hydrophilic property as well as reduced size and uniform morphology. In recent years, a variety of methods have been successfully applied to the synthesis of ferrites, such as ball-milling, 12 co-precipitation, 13 ultrasound irradiation, 14 reverse micelle synthesis, 15 pulsed laser decomposition, high-temperature thermal decomposition, 16 hydrothermal synthesis, 17 sol-gel method, 18 and polymerized complex method.…”