Regioselective synthesis of 6’’-<i>O</i>-lauroyl-1-kestose and 6’’’-<i>O</i>-lauroylnystose by sequential enzymatic reactions of transfructosylation and acylation

Campos-Valdez, Amador Roberto; Casas-Godoy, Leticia; Sandoval, Georgina; Hernández, Lázaro; Sassaki, Guilherme Lanzi; Menezes, Leociley Rocha Alencar; Campos‐Terán, José; Reyes‐Duarte, Dolores; Arrizón, Javier

doi:10.1080/10242422.2021.1952192

Cited by 4 publications

(2 citation statements)

References 28 publications

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“…The mobile phase was methanol–water 90:10 at 0.6 mL/min. FOS are not completely soluble in the reactional system; however, once converted to bioconjugates, their solubility increased as the acylation reaction proceeded, as that kind of compound also has emulsification properties [ 21 , 25 , 43 ]. At the end of reaction, the immobilized lipase was filtered and the reaction solvent was eliminated in a Buchi TM rotary evaporator.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Agave Fructan Bioconjugates on Metabolic Syndrome Parameters in a Murine Model

2023

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Metabolic syndrome is a complex disorder that combines abdominal obesity, dyslipidemia, hypertension, and insulin resistance. Metabolic syndrome affects 25% of the world’s population. Agave fructans have shown positive effects on alterations related to metabolic syndrome, so some investigations have focused on their bioconjugation with fatty acids to increase their biological activity. The objective of this work was to evaluate the effect of agave fructan bioconjugates in a rat model with metabolic syndrome. Agave fructans enzymatically bioconjugated (acylated via food-grade lipase catalysis) with propionate or laurate were administered orally for 8 weeks in rats fed a hypercaloric diet. Animals without treatment were used as the control group, as well as animals fed with a standard diet. The data indicate that the group of animals treated with laurate bioconjugates showed a significant decrease in glucose levels, systolic pressure, weight gain, and visceral adipose tissue, as well as a positive effect of pancreatic lipase inhibition. These results allow us to demonstrate the potential of agave bioconjugates, particularly laurate bioconjugates, for the prevention of diseases associated with metabolic syndrome.

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Section: Methodsmentioning

confidence: 99%

“…Then, 1 mL of this mixture was deposited on the target plate and dried at room temperature. The equipment was calibrated from 380 to 3000 m / z with 1-kestose as the standard [ 43 , 44 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of Agave Fructan Bioconjugates on Metabolic Syndrome Parameters in a Murine Model

2023

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Harvey

2024

Mass Spectrometry Reviews

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The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022. As with previous review, this review also includes a few papers that describe methods appropriate to analysis by MALDI, such as sample preparation, even though the ionization method is not MALDI. The review follows the same format as previous reviews. It is divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of computer software for structural identification. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other general areas such as medicine, industrial processes, natural products and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. MALDI is still an ideal technique for carbohydrate analysis, particularly in its ability to produce single ions from each analyte and advancements in the technique and range of applications show little sign of diminishing.

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Lipase-catalyzed synthesis of laurate esters from puerarin and its β-D-fructofuranosyl-(2→6)-puerarin derivative

Campos,

Casas-Godoy,

Torres-Tolentino

et al. 2024

Biocatalysis and Biotransformation

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Regioselective synthesis of 6’’-O-lauroyl-1-kestose and 6’’’-O-lauroylnystose by sequential enzymatic reactions of transfructosylation and acylation

Cited by 4 publications

References 28 publications

Effect of Agave Fructan Bioconjugates on Metabolic Syndrome Parameters in a Murine Model

Effect of Agave Fructan Bioconjugates on Metabolic Syndrome Parameters in a Murine Model

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Lipase-catalyzed synthesis of laurate esters from puerarin and its β-D-fructofuranosyl-(2→6)-puerarin derivative

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