Regional distribution of neuropeptide processing endopeptidases in adult rat brain

Purification and Characterization of a Dynorphin-processing Endopeptidase

“…In the brain and pituitary, the distribution of the DCE activity generally matches that of Dyn B-13 (10,11). These data suggest that DCE is physiologically involved in the processing of dynorphin peptides at single arginine cleavage sites.…”

Purification and Characterization of a Dynorphin-processing Endopeptidase

“…In the brain and pituitary, the distribution of the DCE activity generally matches that of Dyn B-13 (10,11). These data suggest that DCE is physiologically involved in the processing of dynorphin peptides at single arginine cleavage sites.…”

Minimizing Postsampling Degradation of Peptides by a Thermal Benchtop Tissue Stabilization Method

“…Stabilized hypothalamus had higher DYNB and lower LARG levels. LARG is generated through enzymatic cleavage of dynorphins, 1 , 19 and it is therefore assumed that heat-inactivated enzymes and delayed postmortem turnover resulted in increased substrate levels and decreased product. These findings are in line with a study using in vivo microwave irradiation to stabilize brain tissue.…”

“…This is not surprising considering the tissue-specific processing of endogenous opioids. 19 , 20 Stabilization affects all enzymatic processes, both degradation and processing from precursors. Furthermore, our findings underline the high efficacy of stabilization and imply that rapid freezing after sample collection can be omitted, greatly simplifying the experiment.…”

Purification and Characterization of a Dynorphin-processing Endopeptidase

“…In the brain and pituitary, the distribution of the DCE activity generally matches that of Dyn B-13 (10,11). These data suggest that DCE is physiologically involved in the processing of dynorphin peptides at single arginine cleavage sites.…”

Minimizing Postsampling Degradation of Peptides by a Thermal Benchtop Tissue Stabilization Method

“…Stabilized hypothalamus had higher DYNB and lower LARG levels. LARG is generated through enzymatic cleavage of dynorphins, 1 , 19 and it is therefore assumed that heat-inactivated enzymes and delayed postmortem turnover resulted in increased substrate levels and decreased product. These findings are in line with a study using in vivo microwave irradiation to stabilize brain tissue.…”

“…This is not surprising considering the tissue-specific processing of endogenous opioids. 19 , 20 Stabilization affects all enzymatic processes, both degradation and processing from precursors. Furthermore, our findings underline the high efficacy of stabilization and imply that rapid freezing after sample collection can be omitted, greatly simplifying the experiment.…”

Purification and Characterization of a Dynorphin-processing Endopeptidase

“…In the brain and pituitary, the distribution of the DCE activity generally matches that of Dyn B-13 (10,11). These data suggest that DCE is physiologically involved in the processing of dynorphin peptides at single arginine cleavage sites.…”

Minimizing Postsampling Degradation of Peptides by a Thermal Benchtop Tissue Stabilization Method

“…Stabilized hypothalamus had higher DYNB and lower LARG levels. LARG is generated through enzymatic cleavage of dynorphins, 1 , 19 and it is therefore assumed that heat-inactivated enzymes and delayed postmortem turnover resulted in increased substrate levels and decreased product. These findings are in line with a study using in vivo microwave irradiation to stabilize brain tissue.…”

“…This is not surprising considering the tissue-specific processing of endogenous opioids. 19 , 20 Stabilization affects all enzymatic processes, both degradation and processing from precursors. Furthermore, our findings underline the high efficacy of stabilization and imply that rapid freezing after sample collection can be omitted, greatly simplifying the experiment.…”