Regeneration of transgenic, microspore-derived, fertile barley

Jähne, A.; Becker, Dirk; Brettschneider, Reinhold; Lörz, Horst

doi:10.1007/bf00225390

Cited by 174 publications

(54 citation statements)

References 32 publications

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“…In cereals, green plantlets can be successfully developed from a variety of explants, including embryos (Chauhan et al, 2007), mature embryos (Cho et al, 2004), leaves (Chugh and Khurana, 2003) or seedling segments (Becher et al, 1992), apical meristems (Goldman et al, 2003), coleoptiles (Sahrawat and Chand, 2004), and microspores (Jähne et al, 1994). For barley, morphogenic calli can be induced more frequently from immature embryos (Caligari et al, 1987;Bregitzer et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Genotypic differences in callus induction and plant regeneration from mature embryos of barley (Hordeum vulgare L.)

Han

Jin

et al. 2011

J. Zhejiang Univ. Sci. B

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An efficient induction system and regeneration protocol based on mature barley embryos were developed. Embryos isolated from mature seeds, dehusked by hand and inoculated with longitudinally bisected sections, showed low contamination and high primary callus-forming capability. The influences of nine culture media on primary callus induction and germination from the mature embryos of barley cultivars Golden Promise and Zaoshu 3 were analyzed. The results showed that the two cultivars had much higher values of primary callus induction in the B16M6D medium as compared to the other eight medium formulations, with a frequency of 74.3% and 78.4% for Golden Promise and Zaoshu 3, respectively. Furthermore, Zaoshu 3 demonstrated particularly high stability in callus induction over the different media, indicating its potential utilization in callus induction and regeneration for its good agronomic traits and wide adaption. There were significant differences amongst 11 barley genotypes in terms of primary callus induction in the optimum medium, with percentages of callus induction and germination response ranging from 17.9% to 78.4% and 2.8% to 47.4%, respectively. Green plantlets of Dong 17, Golden Promise, and Zaoshu 3 were successfully developed from primary calli through embryogenesis, with green plant differentiation frequencies ranging from 9.7% to 21.0% across genotypes.

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Section: Introductionmentioning

confidence: 99%

Genotypic differences in callus induction and plant regeneration from mature embryos of barley (Hordeum vulgare L.)

Han

Jin

et al. 2011

J. Zhejiang Univ. Sci. B

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“…All of these factors have resulted in little progress being made towards the development of robust transformation protocols based on immature pollen as explant. Nevertheless, stably transgenic barley plants have been obtained through biolistic transformation of immature pollen followed by embryonic development (Jaehne et al 1994, Carlson et al 2001. Shim et al (2009) were able to show that about one third of the transgenic regenerants derived from biolistic gene transfer to immature barley pollen were instantly homozygous for the transgene, one third were hemizygous, and the remainder (haploids) were successfully converted to homozygous transgenics via colchicine-induced chromosome doubling.…”

Section: Isolated Immature Pollen and Embryogenic Pollenmentioning

confidence: 99%

Genetic transformation technology in the Triticeae

Kumlehn

Hensel

2009

Breed. Sci.

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The Triticeae crops comprise wheat, barley, rye and triticale, which together provide a major portion of the world's food and feed. The growing demand for human nutrition and renewable energy requires an intensification in application-oriented research and the establishment and utilization of current biotechnology in these crops. Genetic transformation provides an important means both to elucidate gene function, and to engineer crop plants in a directed and precise way. This review covers a range of issues surrounding the production of stable transgenic lines within the Triticeae. Some quality aspects of transgenesis of particular relevance to the Triticeae are also discussed.

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“…Macroarray studies yield a wealth of information on the induction of barley microspore embryogenesis Different types of stress treatments are able to trigger the switch of barley microspore development from the gametophytic pathway towards the sporophytic route, including temperature-, osmotic-and starvation-stress (Jä hne et al 1994). Despite the fact that many stress treatments lead to the re-programming of microspores into embryos, a combination of starvation and osmotic stress (mannitol treatment) has been the method of choice in studies aimed at identifying genes involved in the induction of barley microspore embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

Functional genomics of microspore embryogenesis

Hosp

Maraschin²,

Touraev

et al. 2006

Euphytica

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Isolated plant microspores, when stressed and cultured in vitro, can be diverted from their normal gametophytic pathway towards sporophytic development, with the formation of haploid embryos and ultimately doubled-haploid plants. This process is called androgenesis or microspore embryogenesis, and is widely used in plant breeding programmes to generate homozygous lines for breeding purposes. Protocols for the induction of microspore embryogenesis and the subsequent regeneration of doubled haploid (DH) plants have been successfully developed for more than 200 species. These practical advances stand in stark contrast to our knowledge of the underlying molecular genetic mechanism controlling this process. The majority of information regarding the genetic and molecular control of the developmental switch from gametophytic to sporophytic development has been garnered from four intensely studied (crop) plants comprising two dicotyledonous species, rapeseed (Brassica napus) and tobacco (Nicotiana tabacum), and two monocotyledonous species, wheat (Triticum aestivum) and barley (Hordeum vulgare). In these species the efficiency of microspore embryogenesis is very high and reproducible, making them suitable models for molecular studies. In the past, molecular studies on microspore embryogenesis have focussed mainly on the identification of genes that are differentially expressed during this developmental transition and/or early in embryo development, and have identified a number of genes whose expression marks or predicts the developmental fate of stressed microspores. More recently, functional genomics approaches have been used to obtain a broad overview of the molecular processes that take place during the establishment of microspore embryogenesis. In this review we summarise accumulated molecular data obtained in rapeseed, tobacco, wheat and barley on embryogenic induction of microspores and define common aspects involved in the androgenic switch.

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Regeneration of transgenic, microspore-derived, fertile barley

Cited by 174 publications

References 32 publications

Genotypic differences in callus induction and plant regeneration from mature embryos of barley (Hordeum vulgare L.)

Genotypic differences in callus induction and plant regeneration from mature embryos of barley (Hordeum vulgare L.)

Genetic transformation technology in the Triticeae

Functional genomics of microspore embryogenesis

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