Regeneration of the neurogliovascular unit visualized in vivo by transcranial live-cell imaging

Arango-Lievano, Margarita; Dromard, Yann; Fontanaud, Pierre; Lafont, Chrystel; Mollard, Patrice; Jeanneteau, Freddy

doi:10.1016/j.jneumeth.2020.108808

Cited by 3 publications

(3 citation statements)

References 99 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Each scan stacks consists of images at 512 × 512 pixels resolution. Time-lapse acquisition was done in a smaller field of view with galvanometric scanning mode and conventional raster scanning for blood flow measurements based on the line scanning method [ 4 ]. Plasma is fluorescent unlike blood cells that do not uptake dextran dyes permitting identification of their circulation.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Loss of glucocorticoid receptor phosphorylation contributes to cognitive and neurocentric damages of the amyloid-β pathway

Dromard

Arango-Lievano

Borie

et al. 2022

acta neuropathol commun

Self Cite

View full text Add to dashboard Cite

Aberrant cortisol and activation of the glucocorticoid receptor (GR) play an essential role in age-related progression of Alzheimer's disease (AD). However, the GR pathways required for influencing the pathobiology of AD dementia remain unknown. To address this, we studied an early phase of AD-like progression in the well-established APP/PS1 mouse model combined with targeted mutations in the BDNF-dependent GR phosphorylation sites (serines 134/267) using molecular, behavioral and neuroimaging approaches. We found that disrupting GR phosphorylation (S134A/S267A) in mice exacerbated the deleterious effects of the APP/PS1 genotype on mortality, neuroplasticity and cognition, without affecting either amyloid-β deposition or vascular pathology. The dynamics, maturation and retention of task-induced new dendritic spines of cortical excitatory neurons required GR phosphorylation at the BDNF-dependent sites that amyloid-β compromised. Parallel studies in postmortem human prefrontal cortex revealed AD subjects had downregulated BDNF signaling and concomitant upregulated cortisol pathway activation, which correlated with cognitive decline. These results provide key evidence that the loss of neurotrophin-mediated GR phosphorylation pathway promotes the detrimental effects of the brain cortisol response that contributes to the onset and/or progression of AD dementia. These findings have important translational implications as they provide a novel approach to treating AD dementia by identifying drugs that increase GR phosphorylation selectively at the neurotrophic sites to improve memory and cognition.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The number of axonal dystrophies and dendritic spines were expressed as densities. Dextran-Texas Red filled microcirculation but did not penetrate blood cells permitting identification of their circulation as previously described [ 4 ]. The change of flow between imaging sessions was determined only in amyloid-covered vessels.…”

Section: Methodsmentioning

confidence: 99%

Loss of glucocorticoid receptor phosphorylation contributes to cognitive and neurocentric damages of the amyloid-β pathway

Dromard

Arango-Lievano

Borie

et al. 2022

acta neuropathol commun

Self Cite

View full text Add to dashboard Cite

show abstract

“…A multimodal imaging approach was described to visualize NG2-tdTomato-labeled pericytes in mouse brains. Transcranial two-photon microscopy with a 3D imaging volume of 500 × 500 × 250 µm combined with transcranial epifluorescence time-lapse microscopy allowed the study of pericyte turnover after seizure induction in mice and treatment with PDGF-BB [161]. The fluorescent Nissl dye NeuroTrace 500/525 specifically labels αSMA-negative pericytes in brain capillaries in vivo, thus enabling in vivo imaging of selected pericyte subpopulations devoid of αSMA in mouse brains [67].…”

Section: In Vivo Monitoring Of Pericytesmentioning

confidence: 99%

Brain Microvascular Pericytes—More than Bystanders in Breast Cancer Brain Metastasis

Ippolitov

Arreza

Munir

et al. 2022

Cells

View full text Add to dashboard Cite

Brain tissue contains the highest number of perivascular pericytes compared to other organs. Pericytes are known to regulate brain perfusion and to play an important role within the neurovascular unit (NVU). The high phenotypic and functional plasticity of pericytes make this cell type a prime candidate to aid physiological adaptations but also propose pericytes as important modulators in diverse pathologies in the brain. This review highlights known phenotypes of pericytes in the brain, discusses the diverse markers for brain pericytes, and reviews current in vitro and in vivo experimental models to study pericyte function. Our current knowledge of pericyte phenotypes as it relates to metastatic growth patterns in breast cancer brain metastasis is presented as an example for the crosstalk between pericytes, endothelial cells, and metastatic cells. Future challenges lie in establishing methods for real-time monitoring of pericyte crosstalk to understand causal events in the brain metastatic process.

show abstract

Mediterranean Neuroscience Methods 2019

Deurwaerdère

Galati²,

Giovanni

2021

Journal of Neuroscience Methods

View full text Add to dashboard Cite

Regeneration of the neurogliovascular unit visualized in vivo by transcranial live-cell imaging

Cited by 3 publications

References 99 publications

Loss of glucocorticoid receptor phosphorylation contributes to cognitive and neurocentric damages of the amyloid-β pathway

Loss of glucocorticoid receptor phosphorylation contributes to cognitive and neurocentric damages of the amyloid-β pathway

Brain Microvascular Pericytes—More than Bystanders in Breast Cancer Brain Metastasis

Mediterranean Neuroscience Methods 2019

Contact Info

Product

Resources

About