Regeneration of Full-Thickness Skin Defects Using Umbilical Cord Blood Stem Cells Loaded into Modified Porous Scaffolds

Abstract

In this study, we have demonstrated the ability of cord blood (CB)-derived unrestricted somatic stem cells (USSCs) and chitosan-modified poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffold to promote skin regeneration. Afterward, the scaffolds were evaluated by structural, microscopic, physical, and mechanical assays and cell culture analyses. Results of structural, physical, and mechanical analyses also showed a good resilience and compliance with movement as a skin graft. Cellular experiments showed a better cell adhesion, growth, and proliferation inside the modified scaffolds compared with unmodified ones. In animal models with histological examinations, all groups, excluding the control group especially the groups treated with stem cells, exhibited the most pronounced effect on wound closure, with the statistically significant improvement in wound healing being seen at postoperative day 21. These data suggest that chitosan-modified PHBV scaffold loaded with CB-derived USSCs could significantly contribute to wound repair and be potentially used in the tissue engineering.

Surface Modification of PHBV Nanofibrous Mat by Laminin Protein and Its Cellular Study

“…To achieve positive cellbiomaterial interaction, functional groups or biomacromolecules have been immobilized onto PHAs for direct tuning of surface without altering their bulk properties [1][2][3][4][5][6][7][8]. Electrospinning has been rapidly developed into a technique to prepare nanofibers with the diameter ranging from tens of nanometers to several microns [9][10][11][12][13][14][15][16][17][18][19][20][21][22]. Controlling surface properties is very important for the high performance of adhesion.…”

New advances in the mesenchymal stem cells therapy against skin flaps necrosis

“…Recently, tissue engineered skin graft develops rapidly when taking micro-environment into consideration. Chitosan-modified poly(3-hydroxybutyrate-co-3-hydroxyvalerate) scaffold loaded with HUCMSCs or unrestricted somatic stem cells could significantly contribute to fullthickness skin defects repair and be potentially used in the tissue engineering [50,51] . Laser microporous porcine acellular dermal matrix, which provided a "cell niche-like" micro-environment for the migration and differentiation of the BMSCs population, could induce exogenous differentiation of BMSCs in vivo and achieve the reconstruction of skin appendages, when combining with the splitthickness skin graft [52] .…”

Electrospun Poly (3-Hydroxybutyrate-co-3-Hydroxyvalerate)/Hydroxyapatite Scaffold With Unrestricted Somatic Stem Cells for Bone Regeneration

“…Only several PHAs, including poly 3-hydroxybutyrate (PHB), copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate (PHBV), poly 4-hydroxybutyrate (P4HB), copolymers of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx), and poly 3-hydroxyoctanoate (PHO), are available in sufficient quantity for application research. [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] Hydroxyapatite [HAp, Ca 10 (PO 4 ) 6 (OH) 2 ] is a type of calcium phosphates, which has extensive applications in the healing of bone and tooth, because of biocompatibility and similar composition to natural bone. 30,31 In the current study, unrestricted somatic stem cells (USSCs) were used as a regenerative cell for the growth and repairing of bone defect.…”