Regeneration: making muscle from hPSCs

Zhu, Xiping; Fu, Ling; Yi, Fei; Liu, Guanghui; Ocampo, Alejandro; Qu, Jing; Belmonte, Juan Carlos Izpisúa

doi:10.1038/cr.2014.91

Cited by 6 publications

(5 citation statements)

References 11 publications

(22 reference statements)

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“…In recent years, several protocols have been reported to propagate human myogenic progenitors from pluripotent cell sources and to differentiate these progenitors into the skeletal muscle cell lineage as myoblasts or myotubes (Zhu et al, 2014). While many protocols require cell sorting and/or rely on exogenous expression of myogenic genes such as PAX3, PAX7, and MYOD (Abujarour et al, 2014; Darabi et al, 2012; Maffioletti et al, 2015; Skoglund et al, 2014; Tanaka et al, 2013), more recent advances have been made with the application of small molecules and growth factors to directly promote myogenic differentiation from human iPSCs (Barberi et al, 2007; Borchin et al, 2013; Caron et al, 2016; Chal et al, 2016; Chal et al, 2015; Choi et al, 2016; Hosoyama et al, 2014; Hwang et al, 2013; Shelton et al, 2014; Xu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Differentiation and sarcomere formation in skeletal myocytes directly prepared from human induced pluripotent stem cells using a sphere-based culture

et al. 2017

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Human induced-pluripotent stem cells (iPSCs) are a promising resource for propagation of myogenic progenitors. Our group recently reported a unique protocol for the derivation of myogenic progenitors directly (without genetic modification) from human pluripotent cells using free-floating spherical culture. Here we expand our previous efforts and attempt to determine how differentiation duration, culture surface coatings, and nutrient supplements in the medium influence progenitor differentiation and formation of skeletal myotubes containing sarcomeric structures. A long differentiation period (over 6 weeks) promoted the differentiation of iPSC-derived myogenic progenitors and subsequent myotube formation. These iPSC-derived myotubes contained representative sarcomeric structures, consisting of organized myosin and actin filaments, and could spontaneously contract. We also found that a bioengineering approach using three-dimensional (3D) artificial muscle constructs could facilitate the formation of elongated myotubes. Lastly, we determined how culture surface coating matrices and different supplements would influence terminal differentiation. While both Matrigel and laminin coatings showed comparable effects on muscle differentiation, B27 serum-free supplement in the differentiation medium significantly enhanced myogenesis compared to horse serum. Our findings support the possibility to create an in vitro model of contractile sarcomeric myofibrils for disease modeling and drug screening to study neuromuscular diseases.

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Section: Introductionmentioning

confidence: 99%

Differentiation and sarcomere formation in skeletal myocytes directly prepared from human induced pluripotent stem cells using a sphere-based culture

et al. 2017

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“…Despite the progress that has been made with rodent cells, the current methods used to expand primary, human muscle stem cells in vitro remain inefficient and, thus, the use of human induced pluripotent stem cells (iPSCs) holds the promise of providing unlimited numbers of patient-specific myogenic cells for disease modeling and autologous cell therapies (101). During the past 3 years, myogenic cells have been derived from human iPSCs from normal individuals and those with different congenital muscle diseases by forcing expression of muscle transcription factors PAX7 (102, 103) or MYOD (104–107) using doxycycline-inducible lentiviral and transposon vectors.…”

Section: Bioengineering Methods For Skeletal Muscle Research and Rmentioning

confidence: 99%

Synergizing Engineering and Biology to Treat and Model Skeletal Muscle Injury and Disease

Bursac

Juhas

Rando

2015

Annu. Rev. Biomed. Eng.

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Although skeletal muscle is one of the most regenerative organs in our body, various genetic defects, alterations in extrinsic signaling, or substantial tissue damage can impair muscle function and the capacity for self-repair. The diversity and complexity of muscle disorders have attracted much interest from both cell biologists and, more recently, bioengineers, leading to concentrated efforts to better understand muscle pathology and develop more efficient therapies. This review describes the biological underpinnings of muscle development, repair, and disease, and discusses recent bioengineering efforts to design and control myomimetic environments, both to study muscle biology and function and to aid in the development of new drug, cell, and gene therapies for muscle disorders. The synergy between engineering-aided biological discovery and biology-inspired engineering solutions will be the path forward for translating laboratory results into clinical practice.

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“…Other methods for inducing myogenic differentiation have been demonstrated in cultured mammalian cells (Zhu et al 2014 ) but not reported in fish. The reported strategies rely on myogenic inducers such as a specific skeletal muscle cell growth medium (SkGM™)-2 BulletKit™ (Lonza) containing human epidermal growth factor (hEGF), fetuin, FBS, dexamethasone, and insulin, supplemented with 5-azacytidine (Stern-Straeter et al 2014 ; Okamura et al 2018 ).…”

Section: Myogenesis In Fishmentioning

confidence: 99%

Differentiation and Maturation of Muscle and Fat Cells in Cultivated Seafood: Lessons from Developmental Biology

Bomkamp¹,

Musgrove

Marques

et al. 2022

Mar Biotechnol

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Cultivated meat, also known as cultured or cell-based meat, is meat produced directly from cultured animal cells rather than from a whole animal. Cultivated meat and seafood have been proposed as a means of mitigating the substantial harms associated with current production methods, including damage to the environment, antibiotic resistance, food security challenges, poor animal welfare, and—in the case of seafood—overfishing and ecological damage associated with fishing and aquaculture. Because biomedical tissue engineering research, from which cultivated meat draws a great deal of inspiration, has thus far been conducted almost exclusively in mammals, cultivated seafood suffers from a lack of established protocols for producing complex tissues in vitro. At the same time, fish such as the zebrafish Danio rerio have been widely used as model organisms in developmental biology. Therefore, many of the mechanisms and signaling pathways involved in the formation of muscle, fat, and other relevant tissue are relatively well understood for this species. The same processes are understood to a lesser degree in aquatic invertebrates. This review discusses the differentiation and maturation of meat-relevant cell types in aquatic species and makes recommendations for future research aimed at recapitulating these processes to produce cultivated fish and shellfish.

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Regeneration: making muscle from hPSCs

Cited by 6 publications

References 11 publications

Differentiation and sarcomere formation in skeletal myocytes directly prepared from human induced pluripotent stem cells using a sphere-based culture

Differentiation and sarcomere formation in skeletal myocytes directly prepared from human induced pluripotent stem cells using a sphere-based culture

Synergizing Engineering and Biology to Treat and Model Skeletal Muscle Injury and Disease

Differentiation and Maturation of Muscle and Fat Cells in Cultivated Seafood: Lessons from Developmental Biology

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