Reflective imaging improves resolution, speed, and collection efficiency in light sheet microscopy

Abstract

Light-sheet fluorescence microscopy (LSFM) enables high-speed, high-resolution, gentle imaging of live biological specimens over extended periods. Here we describe a technique that improves the spatiotemporal resolution and collection efficiency of LSFM without modifying the underlying microscope. By imaging samples on reflective coverslips, we enable simultaneous collection of multiple views, obtaining 4 complementary views in 250 ms, half the period it would otherwise take to collect only two views in symmetric dual-view selective plane illumination microscopy (diSPIM). We also report a modified deconvolution algorithm that removes the associated epifluorescence contamination and fuses all views for resolution recovery. Furthermore, we enhance spatial resolution (to < 300 nm in all three dimensions) by applying our method to a new asymmetric diSPIM, permitting simultaneous acquisition of two high-resolution views otherwise difficult to obtain due to steric constraints at high numerical aperture (NA). We demonstrate the broad applicability of our method in a variety of samples of moderate (< 50 µm) thickness, studying mitochondrial, membrane, Golgi, and microtubule dynamics in single cells and calcium activity in nematode embryos.

Single-fluorophore orientation determination with multiview polarized illumination: modeling and microscope design

“…In all cases we consider the effect of varying the illumination and detection numerical aperture. We also consider asymmetric NA designs (see [21] for an asymmetric NA design) and the effect of asymmetric sample exposures. Note that we use the word symmetry to refer to dual-view designs with identical objectives and sample exposures and the word asymmetry to refer to dual-view designs with objectives that have a different NA or sample exposure.…”

“…Recently, there has been increasing interest in multiview microscopy techniques for biological imaging [18][19][20][21]. Multiview microscopes offer two major advantages over single-view microscopes.…”