Reflectance-Mode Confocal Microscopy for the In Vivo Detection of Sarcoptes scabiei

Longo, Caterina; Bassoli, Sara; Monari, Paola; Seidenari, Stefania; Pellacani, Giovanni

doi:10.1001/archderm.141.10.1336

Cited by 38 publications

(37 citation statements)

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“…OCT imaging has been reported before in the visualization of infestation with scabies and larva migrans [15,20,21,22,23]. The identification of demodex mites in the hair follicle by OCT has not been reported so far.…”

Section: Discussionmentioning

confidence: 99%

High-Definition Optical Coherence Tomography for the in vivo Detection of Demodex Mites

et al. 2012

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Background: Demodex mites are involved in different skin diseases and are commonly detected by skin scrape tests or superficial biopsies. A new high-definition optical coherence tomography (HD-OCT) with high lateral and axial resolution in a horizontal (en-face) and vertical (slice) imaging mode might offer the possibility of noninvasive and fast in vivo examination of demodex mites. Methods: Twenty patients with demodex-related skin diseases and 20 age- and gender-matched healthy controls were examined by HD-OCT. Mites per follicle and follicles per field of view were counted and compared to skin scrape tests. Results: HD-OCT images depicted mites in the en-face mode as bright round dots in groups of 3–5 mites per hair follicle. In the patients with demodex-related disease, a mean number of 3.4 mites per follicle were detected with a mean number of 2.9 infested follicles per area of view compared to a mean of 0.6 mites in 0.4 infested follicles in the controls. The skin scrape tests were negative in 21% of the patients. Conclusion: The innovative HD-OCT enables fast and noninvasive in vivo recognition of demodex mites and might become a useful tool in the diagnosis and treatment monitoring of demodex-related skin diseases.

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Section: Discussionmentioning

confidence: 99%

High-Definition Optical Coherence Tomography for the in vivo Detection of Demodex Mites

et al. 2012

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“…Longo et al [33] detected by means of RCM a Sarcoptes scabiei of an oval structure with two pairs of denticles. This method also permits visualization of tunnels drilled by the female mite within the stratum corneum.…”

Section: Parasitic Infectionsmentioning

confidence: 99%

Review paper The use of reflectance confocal microscopy in selected inflammatory skin diseases

Białek-Galas¹,

Wielowieyska-Szybińska²,

Dyduch³

et al. 2015

pjp

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Reflectance confocal microscopy is a modern, non-invasive diagnostic method that enables real-time imaging of the epidermis and upper layers of the dermis with nearly histological precision and high contrast. The application of this technology to skin imaging during the last years has resulted in progress of dermatological diagnosis, providing virtual access to living skin, without the need for conventional histopathology. The presented method potentially has broad application in the diagnosis of skin diseases. This article provides a summary of the latest reports and previous achievements in the field of reflectance confocal microscopy. General characteristics of confocal images in selected inflammatory skin diseases are presented.

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“…As a light source, this technique employs a diode laser and combines the advantage of being non-invasive with the ability to study dynamic events occurring within various layers of the skin. Detection of S. scabiei using RCM has been described by some authors in the literature (4)(5)(6)(7)(8)(9)(10)(11). However, to the best of our knowledge, the examination of S. scabiei with RCM in real-time video format has not been reported in Turkey.…”

Section: Introductionmentioning

confidence: 99%

A Case Report of Real-Time in vivo Demonstration of Sarcoptes scabiei

Gürel¹,

Erdemir²,

Tekin³

2018

TurkiyeParazitolDerg

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Scabies is a pruritic dermatosis caused by the ectoparasite Sarcoptes scabiei var. hominis. The diagnosis of scabies is usually made on clinical grounds, but histopathological and/or dermoscopic examinations may sometimes be of assistance. However, these diagnostic modalities do not offer a detailed in vivo demonstration of the motile microorganism. Reflectance confocal microscopy (RCM) is a relatively novel imaging modality that permits in vivo examination of the skin at a resolution similar to that used during similar to histopathologic resolution. Here, a patient with crusted scabies is presented in whom a brief section of the lifecycle of S. scabiei was captured by RCM. Using this advanced imaging modality, the ectoparasite's motion within the human host can be examined for clinical or research purposes and the mite's viability may be assessed to monitor the response to treatment.

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Reflectance-Mode Confocal Microscopy for the In Vivo Detection of Sarcoptes scabiei

Cited by 38 publications

References 0 publications

High-Definition Optical Coherence Tomography for the in vivo Detection of Demodex Mites

High-Definition Optical Coherence Tomography for the in vivo Detection of Demodex Mites

Review paper The use of reflectance confocal microscopy in selected inflammatory skin diseases

A Case Report of Real-Time in vivo Demonstration of Sarcoptes scabiei

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