Refined Candidate Region for F4ab/ac Enterotoxigenic Escherichia coli Susceptibility Situated Proximal to MUC13 in Pigs

Goetstouwers, Tiphanie; Poucke, Mario Van; Coppieters, Wouter; Nguyen, Van Ut; Melkebeek, Vesna; Coddens, Annelies; Steendam, Katleen Van; Deforce, Dieter; Cox, Eric; Peelman, Luc

doi:10.1371/journal.pone.0105013

Cited by 30 publications

(36 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Transmembrane MUC13 prevents ETEC adhesion in pigs [77] and a MUC13 isoform lacking the glycosylated extracellular domain is associated with an increased susceptibility to ETEC [78]. However, some evidence has been published to suggest that, rather than MUC13, it is a neighboring gene region that is involved in the reported ETEC susceptibility [79]. In a 3D villi model with Caco-2 cells, MUC17 reduces infection with Salmonella typhimurium and MUC17 knockdown disrupts tight junction formation and leads to increased Salmonella invasion [80].…”

Section: Barrier Function Mucosal Maintenance and Interactions Withmentioning

confidence: 99%

Transmembrane Mucins: Signaling Receptors at the Intersection of Inflammation and Cancer

Putten¹,

Strijbis²

2017

J Innate Immun

206

213

View full text Add to dashboard Cite

Mucosal surfaces line our body cavities and provide the interaction surface between commensal and pathogenic microbiota and the host. The barrier function of the mucosal layer is largely maintained by gel-forming mucin proteins that are secreted by goblet cells. In addition, mucosal epithelial cells express cell-bound mucins that have both barrier and signaling functions. The family of transmembrane mucins consists of diverse members that share a few characteristics. The highly glycosylated extracellular mucin domains inhibit invasion by pathogenic bacteria and can form a tight mesh structure that protects cells in harmful conditions. The intracellular tails of transmembrane mucins can be phosphorylated and connect to signaling pathways that regulate inflammation, cell-cell interactions, differentiation, and apoptosis. Transmembrane mucins play important roles in preventing infection at mucosal surfaces, but are also renowned for their contributions to the development, progression, and metastasis of adenocarcinomas. In general, transmembrane mucins seem to have evolved to monitor and repair damaged epithelia, but these functions can be highjacked by cancer cells to yield a survival advantage. This review presents an overview of the current knowledge of the functions of transmembrane mucins in inflammatory processes and carcinogenesis in order to better understand the diverse functions of these multifunctional proteins.

show abstract

Section: Barrier Function Mucosal Maintenance and Interactions Withmentioning

confidence: 99%

Transmembrane Mucins: Signaling Receptors at the Intersection of Inflammation and Cancer

Putten¹,

Strijbis²

2017

J Innate Immun

206

213

View full text Add to dashboard Cite

show abstract

“…In differing studies, transcription of either MUC13 or MUC20, another gene associate to F4ac binding (176), did not relate to the adhesive phenotype (177). Moreover, biochemical studies were unable to detect an interaction between MUC13 and F4ac and genotyping assays suggested that a yet uncharacterized M13-adjacent orphan gene participates in glycosylation of the F4ac receptor (178). In another investigation, the SNPs or transcription of 12 genes involved in the assembly of glycosphingolipid carbohydrates could not be associated to a F4 binding phenotype (179).…”

Section: Adhesins and Host Receptorsmentioning

confidence: 99%

Animal EnterotoxigenicEscherichia coli

2016

View full text Add to dashboard Cite

Enterotoxigenic Escherichia coli (ETEC) is the most common cause of E. coli diarrhea in farm animals. ETEC are characterized by the ability to produce two types of virulence factors; adhesins that promote binding to specific enterocyte receptors for intestinal colonization and enterotoxins responsible for fluid secretion. The best-characterized adhesins are expressed in the context of fimbriae, such as the F4 (also designated K88), F5 (K99), F6 (987P), F17 and F18 fimbriae. Once established in the animal small intestine, ETEC produces enterotoxin(s) that lead to diarrhea. The enterotoxins belong to two major classes; heat-labile toxin that consist of one active and five binding subunits (LT), and heat-stable toxins that are small polypeptides (STa, STb, and EAST1). This chapter describes the disease and pathogenesis of animal ETEC, the corresponding virulence genes and protein products of these bacteria, their regulation and targets in animal hosts, as well as mechanisms of action. Furthermore, vaccines, inhibitors, probiotics and the identification of potential new targets identified by genomics are presented in the context of animal ETEC.

show abstract

“…In the study by Goetstouwers et al . (), MUC13 antibodies did not recognize the F4‐specific high molecular weight (MW) glycoproteins under any conditions. Furthermore, the immunoprecipitated MUC13 was not recognized by the F4ac fimbriae.…”

Section: Discussionmentioning

confidence: 93%

“…; Goetstouwers et al . ). Recently, MUC13 was suggested as the causal gene for F4ac susceptibility (Ren et al .…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Effective genetic markers for identifying the Escherichia coli F4ac receptor status of pigs

Rampoldi

Bratus-Neuenschwander

et al. 2019

Animal Genetics

View full text Add to dashboard Cite

The F4ac receptor locus (F4acR), which encodes susceptibility or resistance to Escherichia coli diarrhoea, is inherited as an autosomal recessive monogenetic trait. F4acR is localized on pig chromosome 13 (SSC13q41-q44) near the MUC13 gene. Two flanking markers (CHCF1 and ALGA0106330) with a high linkage disequilibrium (LD) with F4acR were found to be effective for the genetic identification of F4ac-resistant pigs in the Swiss Large White breed (one recombinant out of 2034 genotyped pigs). Three recombinant boars, one each from the Duroc, Swiss Landrace and Pi etrain breeds, were genotyped with seven different markers and phenotyped by means of a microscopic adhesion test. Only ALGA0072075, CHCF1 and CHCF3 indicated the correct phenotype. To test the effect of the resistance allele on production traits, 530 Large White pigs from the national test station were investigated. A significant difference existed among the F4acR locus genotypes in the intramuscular fat content of the longissimus dorsi muscle, whereas no other production traits were influenced by the resistance allele. The frequency of the CHCF1-C and ALGA0106330-A alleles associated with resistance in the Swiss Large White population was 60%, which is advantageous for implementing this trait in a breeding programme to select for E. coli F4ac-resistant animals. The selection of resistant pigs should start on the male side due to the inability of resistant sows to produce sufficient amounts of protecting antibodies in the colostrum. Selection of genetically F4ac-resistant pigs is a sustainable and suitable alternative to decreasing animal loss and antibiotic use due to diarrhoea.

show abstract

Refined Candidate Region for F4ab/ac Enterotoxigenic Escherichia coli Susceptibility Situated Proximal to MUC13 in Pigs

Cited by 30 publications

References 37 publications

Transmembrane Mucins: Signaling Receptors at the Intersection of Inflammation and Cancer

Transmembrane Mucins: Signaling Receptors at the Intersection of Inflammation and Cancer

Animal EnterotoxigenicEscherichia coli

Effective genetic markers for identifying the Escherichia coli F4ac receptor status of pigs

Contact Info

Product

Resources

About