Reduction of Tissue Edema by Microdialysis

The Effect of Capillary Ultrafiltration Probes on Skin Flap Edema

“…In the case of hyperosmolar microdialysis, it was estimated that as much as 100 times more fluid is recovered by the catheters than is removed (net loss) from the tissue. 1 It is likely the same situation with CUP microdialysis in that more fluid is recovered than would be indicated by the observed reduction in tissue water content. New interstitial fluid is delivered to the interstitium by the capillaries at the same time that microdialysis probes remove fluid, which results in considerable fluid flux but a small net decrease in tissue water content.…”

“…An early project studied the use of microdialysis using a hyperosmolar solution of inulin in a semipermeable hollow fiber to produce an osmotic pressure gradient to reduce edema in a skin flap model. Edema was reduced by 1.8 mL of fluid/100 g of wet tissue over controls 1 . This was the first report to our knowledge of microdialysis in which osmotic pressure was used to reduce tissue edema.…”

“…In the case of hyperosmolar microdialysis, it was estimated that as much as 100 times more fluid is recovered by the catheters than is removed (net loss) from the tissue 1 . It is likely the same situation with CUP microdialysis in that more fluid is recovered than would be indicated by the observed reduction in tissue water content.…”

The Effect of Capillary Ultrafiltration Probes on Skin Flap Edema

“…In the case of hyperosmolar microdialysis, it was estimated that as much as 100 times more fluid is recovered by the catheters than is removed (net loss) from the tissue. 1 It is likely the same situation with CUP microdialysis in that more fluid is recovered than would be indicated by the observed reduction in tissue water content. New interstitial fluid is delivered to the interstitium by the capillaries at the same time that microdialysis probes remove fluid, which results in considerable fluid flux but a small net decrease in tissue water content.…”

“…An early project studied the use of microdialysis using a hyperosmolar solution of inulin in a semipermeable hollow fiber to produce an osmotic pressure gradient to reduce edema in a skin flap model. Edema was reduced by 1.8 mL of fluid/100 g of wet tissue over controls 1 . This was the first report to our knowledge of microdialysis in which osmotic pressure was used to reduce tissue edema.…”

“…In the case of hyperosmolar microdialysis, it was estimated that as much as 100 times more fluid is recovered by the catheters than is removed (net loss) from the tissue 1 . It is likely the same situation with CUP microdialysis in that more fluid is recovered than would be indicated by the observed reduction in tissue water content.…”

Interstitial Delivery of Vascular Endothelial Growth Factor to Skin Flaps

“…The punch biopsy specimens were used to determine water content by the wet-dry method. [10][11][12][13] Punch biopsy specimens were weighed immediately after harvest and placed on an impermeable surface in a climatecontrolled laboratory for 1 week, and the dry specimens were weighed again. The difference in wet and dry weight divided by the wet weight was used to calculate the percentage of water content.…”

“…After photographs had been completed, the skin flaps were harvested, and 5-mm punch biopsy specimens were immediately taken from the midline of the flap at 1 cm, 3 cm, 5 cm, and 9 cm from the distal margin of the flap. The punch biopsy specimens were used to determine water content by the wet-dry method 10-13…”

The Effect of Capillary Ultrafiltration Probes on Skin Flap Edema

“…In the case of hyperosmolar microdialysis, it was estimated that as much as 100 times more fluid is recovered by the catheters than is removed (net loss) from the tissue. 1 It is likely the same situation with CUP microdialysis in that more fluid is recovered than would be indicated by the observed reduction in tissue water content. New interstitial fluid is delivered to the interstitium by the capillaries at the same time that microdialysis probes remove fluid, which results in considerable fluid flux but a small net decrease in tissue water content.…”

“…An early project studied the use of microdialysis using a hyperosmolar solution of inulin in a semipermeable hollow fiber to produce an osmotic pressure gradient to reduce edema in a skin flap model. Edema was reduced by 1.8 mL of fluid/100 g of wet tissue over controls 1 . This was the first report to our knowledge of microdialysis in which osmotic pressure was used to reduce tissue edema.…”

“…In the case of hyperosmolar microdialysis, it was estimated that as much as 100 times more fluid is recovered by the catheters than is removed (net loss) from the tissue 1 . It is likely the same situation with CUP microdialysis in that more fluid is recovered than would be indicated by the observed reduction in tissue water content.…”

Interstitial Delivery of Vascular Endothelial Growth Factor to Skin Flaps

“…The punch biopsy specimens were used to determine water content by the wet-dry method. [10][11][12][13] Punch biopsy specimens were weighed immediately after harvest and placed on an impermeable surface in a climatecontrolled laboratory for 1 week, and the dry specimens were weighed again. The difference in wet and dry weight divided by the wet weight was used to calculate the percentage of water content.…”

“…After photographs had been completed, the skin flaps were harvested, and 5-mm punch biopsy specimens were immediately taken from the midline of the flap at 1 cm, 3 cm, 5 cm, and 9 cm from the distal margin of the flap. The punch biopsy specimens were used to determine water content by the wet-dry method 10-13…”

The Effect of Capillary Ultrafiltration Probes on Skin Flap Edema

“…In the case of hyperosmolar microdialysis, it was estimated that as much as 100 times more fluid is recovered by the catheters than is removed (net loss) from the tissue. 1 It is likely the same situation with CUP microdialysis in that more fluid is recovered than would be indicated by the observed reduction in tissue water content. New interstitial fluid is delivered to the interstitium by the capillaries at the same time that microdialysis probes remove fluid, which results in considerable fluid flux but a small net decrease in tissue water content.…”

“…An early project studied the use of microdialysis using a hyperosmolar solution of inulin in a semipermeable hollow fiber to produce an osmotic pressure gradient to reduce edema in a skin flap model. Edema was reduced by 1.8 mL of fluid/100 g of wet tissue over controls 1 . This was the first report to our knowledge of microdialysis in which osmotic pressure was used to reduce tissue edema.…”

“…In the case of hyperosmolar microdialysis, it was estimated that as much as 100 times more fluid is recovered by the catheters than is removed (net loss) from the tissue 1 . It is likely the same situation with CUP microdialysis in that more fluid is recovered than would be indicated by the observed reduction in tissue water content.…”

Interstitial Delivery of Vascular Endothelial Growth Factor to Skin Flaps

“…The punch biopsy specimens were used to determine water content by the wet-dry method. [10][11][12][13] Punch biopsy specimens were weighed immediately after harvest and placed on an impermeable surface in a climatecontrolled laboratory for 1 week, and the dry specimens were weighed again. The difference in wet and dry weight divided by the wet weight was used to calculate the percentage of water content.…”

“…After photographs had been completed, the skin flaps were harvested, and 5-mm punch biopsy specimens were immediately taken from the midline of the flap at 1 cm, 3 cm, 5 cm, and 9 cm from the distal margin of the flap. The punch biopsy specimens were used to determine water content by the wet-dry method 10-13…”