Reduction of the contribution of electrode polarization effects in the radiowave dielectric measurements of highly conductive biological cell suspensions

Bordi, F.; Cametti, C.; Gili, Tommaso

doi:10.1016/s1567-5394(01)00110-4

Cited by 135 publications

(123 citation statements)

References 16 publications

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“…The CPE is often employed to account for EP in ionic conductors [6,7,10,35,39,46]. Its admittance is defined by Y * = A(iν) n with an exponent n < 1 and the prefactor A.…”

Section: Equivalent-circuit Models For Electrode Polarizationmentioning

confidence: 99%

Electrode polarization effects in broadband dielectric spectroscopy

et al. 2011

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In the present work, we provide broadband dielectric spectra showing strong electrode polarization effects for various materials, belonging to very different material classes. This includes both ionic and electronic conductors as, e.g., salt solutions, ionic liquids, human blood, and colossal-dielectricconstant materials. These data are intended to provide a broad data base enabling a critical test of the validity of phenomenological and microscopic models for electrode polarization. In the present work, the results are analyzed using a simple phenomenological equivalent-circuit description, involving a distributed parallel RC circuit element for the modeling of the weakly conducting regions close to the electrodes. Excellent fits of the experimental data are achieved in this way, demonstrating the universal applicability of this approach. In the investigated ionically conducting materials, we find the universal appearance of a second dispersion region due to electrode polarization, which is only revealed if measuring down to sufficiently low frequencies. This indicates the presence of a second charge-transport process in ionic conductors with blocking electrodes.

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“…The CPE is often employed to account for EP in ionic conductors [6,7,10,35,39,46]. Its admittance is defined by Y * = A(iν) n with an exponent n < 1 and the prefactor A.…”

Section: Equivalent-circuit Models For Electrode Polarizationmentioning

confidence: 99%

Electrode polarization effects in broadband dielectric spectroscopy

et al. 2011

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“…Care must be taken to either use experimental setups that mitigate these effects or to use reliable data correction schemes to remove them post-measurement [25][26][27][28].…”

Section: Electrode Polarizationmentioning

confidence: 99%

Permittivity Measurements of Biological Samples by an Open-Ended Coaxial Line

Bobowski¹,

Johnson²

2012

PIER B

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Abstract-We previously reported on the complex permittivity and dc conductivity of waste-activated sludge. The measurements, spanning a frequency range of 3 MHz to 40 GHz, were made using an open-ended coaxial transmission line. Although this technique is well established in the literature, we found that it was necessary to combine methods from several papers to use the open-ended coaxial probe to reliably characterize biological samples having a high dc conductivity. Here, we provide a set of detailed and practical guidelines that can be used to determine the permittivity and conductivity of biological samples over a broad frequency range. Due to the electrode polarization effect, low frequency measurements of conducting samples require corrections to extract the intrinsic electrical properties. We describe one practical correction scheme and verify its reliability using a control sample.

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“…All the circuit elements are functions of the volume fraction or cell size. The electrical double layer (EDL) [91][92][93] has an influence on the measurements at low frequencies (below 1 MHz for high conductivity solutions). This is generally modelled as a constant phase angle (CPA), represented by a resistor (R DL ) and capacitor (C DL ) in series.…”

Section: Modelling and Simulationmentioning

confidence: 99%

“…Practically this is achieved by making electrodes with a large effective surface area. A variety of experimental and/or mathematical correction methods to reduce the effect of the double layer have been reported [91][92][93]. Platinum black or other nano-porous films (e.g.…”

Section: Modelling and Simulationmentioning

confidence: 99%

Single cell dielectric spectroscopy

Morgan

Sun²,

Holmes³

et al. 2006

J. Phys. D: Appl. Phys.

389

313

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Over the last century a number of techniques have been developed which allow the measurement of the dielectric properties of biological particles in fluid suspension. The majority of these techniques are limited by the fact that they only provide an average value for the dielectric properties of a collection of particles. More recently, with the advent of microfabrication techniques and the Lab-on-a-chip, it has been possible to perform dielectric spectroscopic experiments on single biological particles suspended in physiological media. In this paper we review current methods for single cell dielectric spectroscopy. We also discuss alternative single cell dielectric measurement techniques, specifically the ac electrokinetic methods of dielectrophoresis and electrorotation. Single cell electrical impedance spectroscopy is also discussed with relevance to a microfabricated flow cytometer. We compare impedance spectroscopy data obtained from measurements made using a microfabricated flow cytometer with simulation data obtained using an equivalent circuit model for the device.

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Reduction of the contribution of electrode polarization effects in the radiowave dielectric measurements of highly conductive biological cell suspensions

Cited by 135 publications

References 16 publications

Electrode polarization effects in broadband dielectric spectroscopy

Electrode polarization effects in broadband dielectric spectroscopy

Permittivity Measurements of Biological Samples by an Open-Ended Coaxial Line

Single cell dielectric spectroscopy

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